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Anti-Hsp90 beta (phospho S254)抗体(ab51136)

Reviews (1)Q&A (1)References (1)
Western blot - Anti-Hsp90 beta (phospho S254) antibody (ab51136)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta (phospho S254) antibody (ab51136)

Key features and details

  • Rabbit polyclonal to Hsp90 beta (phospho S254)
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-Hsp90 beta (phospho S254)抗体
    参阅全部 Hsp90 beta 一抗

  • 描述

    兔多克隆抗体to Hsp90 beta (phospho S254)

  • 宿主

    Rabbit

  • 特异性

    Detects endogenous levels of HSP90B only when phosphorylated at serine 254.

  • 经测试应用

    适用于: WB, IHC-Pmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide corresponding to Human Hsp90 beta aa 200-300 (phospho S254).
    Database link: P08238

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS

    Without Mg+2 and Ca+2
  • Concentration information loading...

  • 纯度

    Immunogen affinity purified

  • 纯化说明

    The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab51136于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
1/500 - 1/1000. Detects a band of approximately 87 kDa (predicted molecular weight: 83 kDa).
IHC-P
Use at an assay dependent concentration.
说明
WB
1/500 - 1/1000. Detects a band of approximately 87 kDa (predicted molecular weight: 83 kDa).
IHC-P
Use at an assay dependent concentration.

靶标

  • 功能

    Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.

  • 序列相似性

    Belongs to the heat shock protein 90 family.

  • 结构域

    The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins.

  • 翻译后修饰

    Ubiquitinated in the presence of STUB1-UBE2D1 complex (in vitro).
    ISGylated.
    S-nitrosylated; negatively regulates the ATPase activity.

  • 细胞定位

    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • 数据库链接

    • 别名

      • 90 kda heat shock protein beta HSP90 beta antibody
      • D6S182 antibody
      • FLJ26984 antibody
      • Heat shock 84 kDa antibody
      • Heat shock 90kD protein 1, beta antibody
      • Heat shock 90kDa protein 1 beta antibody
      • Heat shock protein 90 alpha family class B member 1 antibody
      • Heat shock protein 90 kDa antibody
      • Heat shock protein 90kDa alpha (cytosolic) class B member 1 antibody
      • Heat shock protein 90kDa alpha family class B member 1 antibody
      • Heat shock protein beta antibody
      • Heat shock protein HSP 90 beta antibody
      • Heat shock protein HSP 90-beta antibody
      • HS90B_HUMAN antibody
      • HSP 84 antibody
      • HSP 90 antibody
      • HSP 90 b antibody
      • HSP 90b antibody
      • HSP84 antibody
      • HSP90 BETA antibody
      • hsp90ab1 antibody
      • HSP90B antibody
      • HSPC2 antibody
      • HSPCB antibody
      see all

    图片

    • Western blot - Anti-Hsp90 beta (phospho S254) antibody (ab51136)
      Western blot - Anti-Hsp90 beta (phospho S254) antibody (ab51136)
      All lanes : Anti-Hsp90 beta (phospho S254) antibody (ab51136) at 1/500 dilution

      Lane 1 : extracts from Hela cells treated with TNF-a with immunizing peptide
      Lane 2 : extracts from Hela cells treated with TNF-a

      Lysates/proteins at 0.02 µg/ml per lane.

      Predicted band size: 83 kDa
      Observed band size: 88 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta (phospho S254) antibody (ab51136)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta (phospho S254) antibody (ab51136)
      Ab51136 at 1/50 dilution staining human breast carcinoma tissue with and without blocking peptide; paraffin embedded.

    文献 (1)

    发表研究结果有使用 ab51136？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab51136 被引用在 1 文献中.

    • Weeraphan C  et al. Phosphoproteome Profiling of Isogenic Cancer Cell-Derived Exosome Reveals HSP90 as a Potential Marker for Human Cholangiocarcinoma. Proteomics 19:e1800159 (2019). PubMed: 31054213

    客户评价及客户问答

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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-Hsp90 beta (phospho S254) antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (colon epithelial cell line)
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Loading amount
    50 µg
    Specification
    colon epithelial cell line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Feb 24 2016

    Question

    Dear Sir or Madam, I am interested in 2 Phosphospecific Antibody ab51136 & ab63562. However, as decribed in Datasheets for these antibodies, the immunogen is: Synthesized phosphopeptide derived from human HSP90B around the phosphorylation site of serine 254 (V-G-SP-D-E) ... or ... of serine 226 (E-I-SP-D-D). Would you please specify more the peptide that was used for immunization? Actually I'd like to use it to detect another homologue of the protein mentioned in datasheet. They have highly similar sequence around this region and it's much better to know if the same peptide for immunization also presents in my protein of interest. Also, please inform me the offer for these 2 antibodies (i.e total price including shpping and handling, as well as how long it takes to get these antibodies after placing order). Thanks a lot, Best regards,

    Read More
    Answer

    Thank you for your inquiry. I can confirm that the immunogen peptide amino acid range for ab51136 is amino acid 245-275 and for ab63562 is amino acid 220-250. I hope this information is helpful and wish you good luck. PS. I send you a quote for both products in a separate email. Both antibodies are in stock at the moment and will be delivered the next day within the EU if ordered before 14:30.

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