Anti-Hsp90 beta抗体[K3705] (ab82522)
![Western blot - Anti-Hsp90 beta antibody [K3705] (ab82522)](https://www.abcam.cn/ps/products/82/ab82522/Images/ab82522-388317-anti-hsp90-beta-antibody-k3705-western-blot-a431-wt-a431-hsp90ab1-knockout-saos2-hl60.jpg "Western blot - Anti-Hsp90 beta antibody [K3705] (ab82522)")
Key features and details
- Mouse monoclonal [K3705] to Hsp90 beta
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human, Chinese hamster
- Isotype: IgM
概述
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产品名称
Anti-Hsp90 beta抗体[K3705]
参阅全部 Hsp90 beta 一抗 -
描述
小鼠单克隆抗体[K3705] to Hsp90 beta -
宿主
Mouse -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Human, Chinese hamster -
免疫原
Recombinant human Hsp90 beta protein
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阳性对照
- WB: A431, Saos-2, HL-60, HeLa, PC-12 cells, CHO-K1 cells and 3T3 cells. IHC-P: Human pancreas FFPE tissue sections.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.1% Sodium azide -
Concentration information loading... -
纯度
Ammonium Sulphate Precipitation -
克隆
单克隆 -
克隆编号
K3705 -
同种型
IgM -
研究领域
相关产品
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Compatible Secondaries
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KO cell lines
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KO cell lysates
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab82522于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB | (1) |
1/1000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
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| 说明 |
|---|
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/1000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa). |
靶标
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功能
Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. -
序列相似性
Belongs to the heat shock protein 90 family. -
结构域
The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins. -
翻译后修饰
Ubiquitinated in the presence of STUB1-UBE2D1 complex (in vitro).
ISGylated.
S-nitrosylated; negatively regulates the ATPase activity. -
细胞定位
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 3326 Human
- Entrez Gene: 15516 Mouse
- Entrez Gene: 301252 Rat
- Omim: 140572 Human
- SwissProt: P08238 Human
- SwissProt: P11499 Mouse
- SwissProt: P34058 Rat
- Unigene: 509736 Human
see all -
别名
- 90 kda heat shock protein beta HSP90 beta antibody
- D6S182 antibody
- FLJ26984 antibody
see all
图片
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Western blot - Anti-Hsp90 beta antibody [K3705] (ab82522)All lanes : Anti-Hsp90 beta antibody [K3705] (ab82522) at 1/1000 dilution
Lane 1 : Wild-type A-431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : HSP90AB1 knockout A-431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : Saos-2 (Human osteosarcoma cell line) whole cell lysate
Lane 4 : HL-60 (Human promyelocytic leukemia cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab82522 observed at 85 kDa. Red - loading control, ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37kDa.
ab82522 was shown to react with Hsp90 beta in wild-type A431 cells in western blot. Loss of signal was observed when HSP90AB1 knockout sample was used. Wild-type A431 and HSP90AB1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab82522 and ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Hsp90 beta antibody [K3705] (ab82522)](https://www.abcam.cn/ps/products/82/ab82522/Images/ab82522-97311-anti-hsp90-beta-antibody-k3705-western-blot.jpg)
Western blot - Anti-Hsp90 beta antibody [K3705] (ab82522)All lanes : Anti-Hsp90 beta antibody [K3705] (ab82522) at 1/1000 dilution
Lane 1 : Molecular weight markers
Lane 2 : Hsp90
Native Human Protein
Lane 3 : Hsp90 alpha Recombinant Human
Protein
Lane 4 : Hsp90 beta Recombinant Human Protein
Lane 5 : HeLa cells
Lane 6 : PC-12 cells
Lane 7 : CHO-K1 cells
Lane 8 : 3T3 cells
Predicted band size: 83 kDa
Observed band size: 83 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [K3705] (ab82522)](https://www.abcam.cn/ps/products/82/ab82522/Images/Hsp90-beta-Primary-antibodies-ab82522-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [K3705] (ab82522)ab82522, at 1/50 dilution, staining Hsp90 beta in human breast cancer tissue by Immunohistochemistry.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab82522 被引用在 4 文献中.
- Samson AL et al. Physicochemical properties that control protein aggregation also determine whether a protein is retained or released from necrotic cells. Open Biol 6:N/A (2016). PubMed: 27810968
- Soleilhavoup C et al. Proteomes of the Female Genital Tract During the Oestrous Cycle. Mol Cell Proteomics 15:93-108 (2016). PubMed: 26518761
- Soleilhavoup C et al. Ram seminal plasma proteome and its impact on liquid preservation of spermatozoa. J Proteomics 109C:245-260 (2014). WB ; Sheep . PubMed: 25053255
- Nemoto T et al. Domain structures and immunogenic regions of the 90-kDa heat-shock protein (HSP90). Probing with a library of anti-HSP90 monoclonal antibodies and limited proteolysis. J Biol Chem 272:26179-87 (1997). PubMed: 9334185