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Signal Transduction Protein Trafficking Chaperones Heat Shock Proteins

Anti-Hsp90 beta抗体(ab2927)

  • Datasheet
  • SDS
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Western blot - Anti-Hsp90 beta antibody (ab2927)
  • Western blot - Anti-Hsp90 beta antibody (ab2927)
  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 beta antibody (ab2927)
  • Western blot - Anti-Hsp90 beta antibody (ab2927)
  • Flow Cytometry - Anti-Hsp90 beta antibody (ab2927)
  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 beta antibody (ab2927)
  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 beta antibody (ab2927)
  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 beta antibody (ab2927)
  • Immunoprecipitation - Anti-Hsp90 beta antibody (ab2927)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody (ab2927)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody (ab2927)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody (ab2927)

Key features and details

  • Rabbit polyclonal to Hsp90 beta
  • Suitable for: ICC/IF, IP, WB, Flow Cyt, IHC-P
  • Reacts with: Mouse, Rat, Human, Non human primates, African green monkey
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-Hsp90 beta antibody [EPR16621] (ab203085)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-Hsp90 beta抗体
    参阅全部 Hsp90 beta 一抗
  • 描述

    兔多克隆抗体to Hsp90 beta
  • 宿主

    Rabbit
  • 特异性

    Detects heat shock protein 90 beta (HSP90). This antibody does not detect HSP86 alpha.
  • 经测试应用

    适用于: ICC/IF, IP, WB, Flow Cyt, IHC-Pmore details
  • 种属反应性

    与反应: Mouse, Rat, Human, Non human primates, African green monkey
    预测可用于: Rabbit, Horse, Cow, Cynomolgus monkey
  • 免疫原

    Synthetic peptide corresponding to Mouse Hsp90 beta aa 2-13.
    Sequence:

    PEEVHHGEEEVE

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • WB: HeLa, MCF7, 293T, K562, A431, HepG2, COS7, NIH3T3 and NRK whole cell lysate. ICC/IF: HepG2, U251, HeLa, NIH3T3 and A2058 cells. Flow Cyt: HeLa cells. IP: HeLa cells. IHC-P: Human tonsil tissue, human placenta tissue, human breast carcinoma tissue.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Heat Shock Proteins

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human Hsp90 beta protein (Active) (ab80033)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2927于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
Use a concentration of 10 - 20 µg/ml.
IP
Use at an assay dependent concentration.

2 μg

WB
1/1000 - 1/20000.
Flow Cyt
Use a concentration of 1 - 20 µg/ml.
IHC-P
Use a concentration of 10 µg/ml.
说明
ICC/IF
Use a concentration of 10 - 20 µg/ml.
IP
Use at an assay dependent concentration.

2 μg

WB
1/1000 - 1/20000.
Flow Cyt
Use a concentration of 1 - 20 µg/ml.
IHC-P
Use a concentration of 10 µg/ml.

靶标

  • 功能

    Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.
  • 序列相似性

    Belongs to the heat shock protein 90 family.
  • 结构域

    The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins.
  • 翻译后修饰

    Ubiquitinated in the presence of STUB1-UBE2D1 complex (in vitro).
    ISGylated.
    S-nitrosylated; negatively regulates the ATPase activity.
  • 细胞定位

    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession P08238 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 767874 Cow
    • Entrez Gene: 3326 Human
    • Entrez Gene: 15516 Mouse
    • Entrez Gene: 301252 Rat
    • Omim: 140572 Human
    • SwissProt: Q76LV1 Cow
    • SwissProt: Q4R4T5 Cynomolgus monkey
    • SwissProt: Q9GKX8 Horse
    • SwissProt: P08238 Human
    • SwissProt: P11499 Mouse
    • SwissProt: P34058 Rat
    • Unigene: 509736 Human
    • Unigene: 2180 Mouse
    • Unigene: 98667 Rat
    see all
  • 别名

    • 90 kda heat shock protein beta HSP90 beta antibody
    • D6S182 antibody
    • FLJ26984 antibody
    • Heat shock 84 kDa antibody
    • Heat shock 90kD protein 1, beta antibody
    • Heat shock 90kDa protein 1 beta antibody
    • Heat shock protein 90 alpha family class B member 1 antibody
    • Heat shock protein 90 kDa antibody
    • Heat shock protein 90kDa alpha (cytosolic) class B member 1 antibody
    • Heat shock protein 90kDa alpha family class B member 1 antibody
    • Heat shock protein beta antibody
    • Heat shock protein HSP 90 beta antibody
    • Heat shock protein HSP 90-beta antibody
    • HS90B_HUMAN antibody
    • HSP 84 antibody
    • HSP 90 antibody
    • HSP 90 b antibody
    • HSP 90b antibody
    • HSP84 antibody
    • HSP90 BETA antibody
    • hsp90ab1 antibody
    • HSP90B antibody
    • HSPC2 antibody
    • HSPCB antibody
    see all

图片

  • Western blot - Anti-Hsp90 beta antibody (ab2927)
    Western blot - Anti-Hsp90 beta antibody (ab2927)
    All lanes : Anti-Hsp90 beta antibody (ab2927)

    Lane 1 : CRISPR targeted HSP90 beta knockout HeLa whole cell lysate
    Lane 2 : Wild-type HeLa whole cell lysate
  • Western blot - Anti-Hsp90 beta antibody (ab2927)
    Western blot - Anti-Hsp90 beta antibody (ab2927)
    All lanes : Anti-Hsp90 beta antibody (ab2927) at 1/1000 dilution

    Lane 1 : MCF7 whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : K562 whole cell lysate
    Lane 5 : A431 whole cell lysate
    Lane 6 : HepG2 whole cell lysate
    Lane 7 : COS7 whole cell lysate
    Lane 8 : NIH3T3 whole cell lysate
    Lane 9 : NRK whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG-HRP at 1/20000 dilution


    Samples were loaded onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was then probed with primary antibody (ab2927) overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with secondary antibody for at least one hour. Chemiluminescent detection was performed using SuperSignal West Pico.

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 beta antibody (ab2927)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 beta antibody (ab2927)

    Immunocytochemistry/Immunofluorescence analysis of HSP90 beta shows staining in HepG2 cells. HSP 90 beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or ab2927 at a dilution of 1:100 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated goat anti-rabbit secondary antibody. Images were taken at 60X magnification.

  • Western blot - Anti-Hsp90 beta antibody (ab2927)
    Western blot - Anti-Hsp90 beta antibody (ab2927)

    Western blot of mouse HSP 86 using ab2927.

  • Flow Cytometry - Anti-Hsp90 beta antibody (ab2927)
    Flow Cytometry - Anti-Hsp90 beta antibody (ab2927)

    Flow cytometry analysis of HSP90 was done on HeLa cells. Cells were fixed, permeabilized and stained with a HSP90 rabbit polyclonal antibody (ab2927) (blue histogram) or a rabbit IgG isotype control (black histogram) at a dilution of 10 µg/mL. After incubation for 1 hour on ice, the cells were labeled with a Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 647 conjugate at a dilution of 1/50 for 1 hour on ice. A representative 10,000 cells were acquired and analyzed for each sample.

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 beta antibody (ab2927)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 beta antibody (ab2927)

    Immunocytochemistry/Immunofluorescence analysis of HSP90 beta shows staining in U251 cells. HSP 90 beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or ab2927 at a dilution of 1:100 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated goat anti-rabbit secondary antibody. Images were taken at 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 beta antibody (ab2927)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 beta antibody (ab2927)

    Immunocytochemistry/Immunofluorescence analysis of HSP90 beta (green) in HeLa and NIH3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% BSA for 15 minutes at room temperature. Cells were incubated with ab2927 at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488 goat-anti-rabbit IgG secondary antibody (1:400) for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye. Images were taken at 20X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 beta antibody (ab2927)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 beta antibody (ab2927)

    Immunocytochemistry/Immunofluorescence analysis of HSP90 beta shows staining in A2058 cells. HSP 90 beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or ab2927 at a dilution of 1:100 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated goat anti-rabbit secondary antibody. Images were taken at 60X magnification.

  • Immunoprecipitation - Anti-Hsp90 beta antibody (ab2927)
    Immunoprecipitation - Anti-Hsp90 beta antibody (ab2927)

    Immunoprecipitation of Hsp90 was performed on HeLa cells. Antigen:antibody complexes were formed by incubating 500µg whole cell lysate with 2µg of Hsp90 polyclonal antibody (ab2927) overnight on a rocking platform at 4°C. Immune complexes were captured on 50µl Protein A/G Agarose washed extensively and eluted with Buffer. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membraneand blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a Hsp90 polyclonal antibody (ab2927) at a dilution of 1:1000 overnight rotating at 4°C, washed in TBSTand probed with HRP detection reagent at a dilution of 1:1000 for at least one hour. Chemiluminescent detection was performed.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody (ab2927)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody (ab2927)
    Immunohistochemistry was performed on normal biopsies of deparaffinized Human tonsil tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a rabbit polyclonal antibody recognizing Heat Shock Protein 84 ab2927 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody (ab2927)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody (ab2927)
    Immunohistochemistry was performed on normal biopsies of deparaffinized Human placenta tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing Heat Shock Protein 84 ab2927 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody (ab2927)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody (ab2927)
    Immunohistochemistry was performed on cancer biopsies of deparaffinized Human breast carcinoma tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a rabbit polyclonal antibody recognizing Heat Shock Protein 84 ab2927 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

实验方案

  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (6)

发表研究结果有使用 ab2927?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab2927 被引用在 6 文献中.

  • Wu Y  et al. The molecular chaperone Hsp90a deficiency causes retinal degeneration by disrupting Golgi organization and vesicle transportation in photoreceptors. J Mol Cell Biol 12:216-229 (2020). PubMed: 31408169
  • Wang Q & Liu X VDAC upregulation and aTAT1-mediated a-tubulin acetylation contribute to tanespimycin-induced apoptosis in Calu-1 cells. Oncol Rep 44:2725-2734 (2020). PubMed: 33125132
  • Wu Y  et al. The molecular chaperone Hsp90 maintains Golgi organization and vesicular trafficking by regulating microtubule stability. J Mol Cell Biol N/A:N/A (2019). PubMed: 31560394
  • Amador-Martínez I  et al. Reduced endothelial nitric oxide synthase activation contributes to cardiovascular injury during chronic kidney disease progression. Am J Physiol Renal Physiol 317:F275-F285 (2019). PubMed: 31116605
  • Lin C  et al. Fever Promotes T Lymphocyte Trafficking via a Thermal Sensory Pathway Involving Heat Shock Protein 90 and a4 Integrins. Immunity 50:137-151.e6 (2019). PubMed: 30650373
  • Rodina A  et al. The epichaperome is an integrated chaperome network that facilitates tumour survival. Nature 538:397-401 (2016). PubMed: 27706135

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