Anti-Hsp90抗体[H90-10] (ab58950)
![Western blot - Anti-Hsp90 antibody [H90-10] (ab58950)](https://www.abcam.cn/ps/products/58/ab58950/Images/ab58950-411668-anti-hsp90-antibody-h90-10-western-blot-hek293t-hek293t-hsp90ab1-knockout-saos2-hl60.jpg "Western blot - Anti-Hsp90 antibody [H90-10] (ab58950)")
Key features and details
- Mouse monoclonal [H90-10] to Hsp90
- Suitable for: WB, Flow Cyt
- Knockout validated
- Reacts with: Human
- Isotype: IgG2a
概述
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产品名称
Anti-Hsp90抗体[H90-10]
参阅全部 Hsp90 一抗 -
描述
小鼠单克隆抗体[H90-10] to Hsp90 -
宿主
Mouse -
特异性
ab58950 recognizes both Hsp90 alpha and Hsp90 beta -
经测试应用
适用于: WB, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Purified full length human Hsp90 beta
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阳性对照
- WB: HEK-293T, Saos-2 and HL-60 cell lysates; Heat-shocked HeLa cell lysate. Flow Cyt: HeLa cells.
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常规说明
Dilute in PBS or medium which is identical to that used in the assay system.
This product was changed from ascites to tissue culture supernatant on 19/12/2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Constituent: 99% PBS -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
Purified from TCS -
克隆
单克隆 -
克隆编号
H90-10 -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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KO cell lines
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KO cell lysates
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab58950于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa).
|
| Flow Cyt |
Use 0.01-0.1µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa). |
|
Flow Cyt
Use 0.01-0.1µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. -
序列相似性
Belongs to the heat shock protein 90 family. -
结构域
The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins like the co-chaperone STUB1. -
翻译后修饰
ISGylated.
S-nitrosylated; negatively regulates the ATPase activity and the activation of eNOS by HSP90AA1. -
细胞定位
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 3320 Human
- Entrez Gene: 3326 Human
- Omim: 140571 Human
- Omim: 140572 Human
- SwissProt: P07900 Human
- SwissProt: P08238 Human
- Unigene: 525600 Human
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别名
- Heat shock 86 kDa antibody
- Heat shock protein 90kDa alpha cytosolic class A member 1 antibody
- Heat shock protein 90kDa alpha cytosolic class B member 1 antibody
see all
图片
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Western blot - Anti-Hsp90 antibody [H90-10] (ab58950)All lanes : Anti-Hsp90 antibody [H90-10] (ab58950) at 0.5 µg/ml
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : HSP90AB1 knockout HEK-293T cell lysate
Lane 3 : Saos-2 cell lysate
Lane 4 : HL-60 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab58950 observed at 85 kDa. Red - loading control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37kDa.
ab58950 was shown to react with Hsp90 in wild-type HEK-293T cells in western blot with loss of signal observed in HSP90AB1 knockout cell line ab266117 (HSP90AB1 knockout cell lysate ab257190). Wild-type and HSP90AB1 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab58950 and ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4°C at 0.5 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Flow Cytometry - Anti-Hsp90 antibody [H90-10] (ab58950)](https://www.abcam.cn/ps/products/58/ab58950/Images/ab58950-2-ab58950FC.jpg)
Flow Cytometry - Anti-Hsp90 antibody [H90-10] (ab58950)Overlay histogram showing HeLa cells stained with ab58950 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58950, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
This image was generated using the ascites version of the product.
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![Western blot - Anti-Hsp90 antibody [H90-10] (ab58950)](https://www.abcam.cn/ps/products/58/ab58950/Images/Hsp90-Primary-antibodies-ab58950-1.jpg)
Western blot - Anti-Hsp90 antibody [H90-10] (ab58950)Image from Niture SK et al, J Biol Chem. 2010 Nov 19;285(47):36865-75. Epub 2010 Sep 23, Fig 6. DOI 10.1074/jbc.M110.175802.Western Blot analysis of the effect of siRNA inhibition of Hsp90 on INrf2. Murine hepatoma (Hepa-1) cells were transfected with control siRNA or Hsp90-specific siRNA and Hsp90 (ab58950), INrf2, and actin levels were analyzed by immunoblotting.
This image was generated using the ascites version of the product.
数据表及文件
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Datasheet download
文献 (3)
ab58950 被引用在 3 文献中.
- Hellbach N et al. Impaired myogenic development, differentiation and function in hESC-derived SMA myoblasts and myotubes. PLoS One 13:e0205589 (2018). PubMed: 30304024
- Shim JS et al. Selective Inhibition of HER2-Positive Breast Cancer Cells by the HIV Protease Inhibitor Nelfinavir. J Natl Cancer Inst 104:1576-90 (2012). Human . PubMed: 23042933
- Niture SK & Jaiswal AK Hsp90 interaction with INrf2(Keap1) mediates stress-induced Nrf2 activation. J Biol Chem 285:36865-75 (2010). WB ; Mouse . PubMed: 20864537
