重组Anti-Hsp90抗体[EPR3953] (ab109248)
![Western blot - Anti-Hsp90 antibody [EPR3953] (ab109248)](https://www.abcam.cn/ps/products/109/ab109248/Images/ab109248-337512-anti-hsp90-antibody-epr3953-western-blot.jpg "Western blot - Anti-Hsp90 antibody [EPR3953] (ab109248)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3953] to Hsp90
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Hsp90抗体[EPR3953]
参阅全部 Hsp90 一抗 -
描述
兔单克隆抗体[EPR3953] to Hsp90 -
宿主
Rabbit -
特异性
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Hsp90 alpha and beta recombinant proteins and HeLa and 293T cell lysates IHC-P: Human gastric adenocarcinoma tissue, Human lung carcinoma and Human kidney tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3953 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109248于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 85 kDa).
|
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| IHC-P |
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250. |
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 85 kDa). |
|
IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250. |
靶标
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功能
Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. -
序列相似性
Belongs to the heat shock protein 90 family. -
结构域
The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins like the co-chaperone STUB1. -
翻译后修饰
ISGylated.
S-nitrosylated; negatively regulates the ATPase activity and the activation of eNOS by HSP90AA1. -
细胞定位
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 3320 Human
- Entrez Gene: 3326 Human
- Entrez Gene: 15516 Mouse
- Entrez Gene: 15519 Mouse
- Entrez Gene: 299331 Rat
- Entrez Gene: 301252 Rat
- Omim: 140571 Human
- Omim: 140572 Human
see all -
别名
- Heat shock 86 kDa antibody
- Heat shock protein 90kDa alpha cytosolic class A member 1 antibody
- Heat shock protein 90kDa alpha cytosolic class B member 1 antibody
see all
图片
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Western blot - Anti-Hsp90 antibody [EPR3953] (ab109248)All lanes : Anti-Hsp90 antibody [EPR3953] (ab109248) at 1/1000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates
Lane 3 : RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates
Lane 4 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 85 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 antibody [EPR3953] (ab109248)](https://www.abcam.cn/ps/products/109/ab109248/Images/ab109248-337383-anti-hsp90-antibody-epr3953-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 antibody [EPR3953] (ab109248)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling Hsp90 with purified ab109248 at 1:50 dilution (19.42 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 antibody [EPR3953] (ab109248)](https://www.abcam.cn/ps/products/109/ab109248/Images/ab109248-121940-anti-hsp90-antibody-epr3953-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 antibody [EPR3953] (ab109248)ab109248, at 1/100, staining Hsp90 in paraffin-embedded Human gastric adenocarcinoma tissue by Immunohistochemistry.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 antibody [EPR3953] (ab109248)](https://www.abcam.cn/ps/products/109/ab109248/Images/ab109248-121939-anti-hsp90-antibody-epr3953-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 antibody [EPR3953] (ab109248)ab109248, at 1/100, staining Hsp90 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-Hsp90 antibody [EPR3953] (ab109248)](https://www.abcam.cn/ps/products/109/ab109248/Images/ab109248-293786-anti-hsp90-antibody-epr3953-western-blot.jpg)
Western blot - Anti-Hsp90 antibody [EPR3953] (ab109248)All lanes : Anti-Hsp90 antibody [EPR3953] (ab109248) at 1/1000 dilution
Lane 1 :Recombinant Human Hsp90 alpha protein (His tag) (ab48801)
Lane 2 :Recombinant human Hsp90 beta protein (Active) (ab80033)
Lysates/proteins at 0.015 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 85 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking and dilution buffer: 5% NFDM/TBST.
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![Anti-Hsp90 antibody [EPR3953] (ab109248)](https://www.abcam.cn/ps/products/109/ab109248/Images/ab109248-8-benefits-of-recombinant-antibodies.png)
Anti-Hsp90 antibody [EPR3953] (ab109248)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab109248 被引用在 7 文献中.
- Sha L et al. Hsp90 inhibitor HSP990 in very low dose upregulates EAAT2 and exerts potent antiepileptic activity. Theranostics 10:8415-8429 (2020). PubMed: 32724478
- Weigand I et al. Impact of USP8 Gene Mutations on Protein Deregulation in Cushing Disease. J Clin Endocrinol Metab 104:2535-2546 (2019). PubMed: 30844069
- Lai R et al. Role of Hsp90/Akt pathway in the pathogenesis of gentamicin-induced hearing loss. Int J Clin Exp Pathol 11:4431-4438 (2018). PubMed: 31949840
- Takata T et al. Generation of glyceraldehyde-derived advanced glycation end-products in pancreatic cancer cells and the potential of tumor promotion. World J Gastroenterol 23:4910-4919 (2017). PubMed: 28785145
- Sha L et al. Pharmacologic inhibition of Hsp90 to prevent GLT-1 degradation as an effective therapy for epilepsy. J Exp Med 214:547-563 (2017). PubMed: 28028152
- Guo W et al. Targeting GRP75 improves HSP90 inhibitor efficacy by enhancing p53-mediated apoptosis in hepatocellular carcinoma. PLoS One 9:e85766 (2014). IHC ; Human . PubMed: 24465691
- Guarani V et al. TIMMDC1/C3orf1 functions as a membrane-embedded mitochondrial complex I assembly factor through association with the MCIA complex. Mol Cell Biol 34:847-61 (2014). PubMed: 24344204