Anti-Hsp90抗体[D7a] (ab59459)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 antibody [D7a] (ab59459)](https://www.abcam.cn/ps/products/59/ab59459/Images/ab59459-450901-AntiHsp90-antibody-IHC.png "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 antibody [D7a] (ab59459)")
Key features and details
- Mouse monoclonal [D7a] to Hsp90
- Suitable for: WB, IHC-P, IHC-Fr, IP, ICC/IF, Flow Cyt
- Knockout validated
- Reacts with: Mouse, Rat, Rabbit, Chicken, Cow, Human, Pig
- Isotype: IgG1
概述
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产品名称
Anti-Hsp90抗体[D7a]
参阅全部 Hsp90 一抗 -
描述
小鼠单克隆抗体[D7a] to Hsp90 -
宿主
Mouse -
特异性
Can isolate complexes of HSP90, Src kinase and cec37
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经测试应用
适用于: WB, IHC-P, IHC-Fr, IP, ICC/IF, Flow Cytmore details -
种属反应性
与反应: Mouse, Rat, Rabbit, Chicken, Cow, Human, Pig -
免疫原
Full length native protein (purified) corresponding to Chicken Hsp90 alpha aa 1-728. Full length protein HSP90 purified from chicken brain
Database link: P11501 -
阳性对照
- WB: HEK-293T, HEK-293, HAP1 and HeLa cell lysates. IHC-P: Human colon cancer tissue. Mouse inflamed colon tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
D7a -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab59459于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (4) |
1/500. Predicted molecular weight: 95 kDa.
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| IHC-P |
1/100000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| IHC-Fr | (1) |
Use at an assay dependent concentration.
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| IP |
Use at an assay dependent concentration.
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| ICC/IF | (4) |
Use at an assay dependent concentration.
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| Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
WB
1/500. Predicted molecular weight: 95 kDa. |
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IHC-P
1/100000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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IHC-Fr
Use at an assay dependent concentration. |
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IP
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. -
序列相似性
Belongs to the heat shock protein 90 family. -
结构域
The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins like the co-chaperone STUB1. -
翻译后修饰
ISGylated.
S-nitrosylated; negatively regulates the ATPase activity and the activation of eNOS by HSP90AA1. -
细胞定位
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 396188 Chicken
- Entrez Gene: 281832 Cow
- Entrez Gene: 767874 Cow
- Entrez Gene: 3320 Human
- Entrez Gene: 3326 Human
- Entrez Gene: 15516 Mouse
- Entrez Gene: 15519 Mouse
- Entrez Gene: 396742 Pig
see all -
别名
- Heat shock 86 kDa antibody
- Heat shock protein 90kDa alpha cytosolic class A member 1 antibody
- Heat shock protein 90kDa alpha cytosolic class B member 1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 antibody [D7a] (ab59459)
Immunohistochemistry analysis of ab59459 staining Hsp90 alpha in mouse inflamed colon tissue section at 1/100000 dilution for 12 hours at 4°C using antibody amplifier™ system. Biotin Goat Anti-Mouse antibody was used at 1:2000 for 1 hour at room tempertature. Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at room temperature was used as a counter stain. Localization: Inflammatory cells. Magnification: 40x.
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![Western blot - Anti-Hsp90 antibody [D7a] (ab59459)](https://www.abcam.cn/ps/products/59/ab59459/Images/ab59459-412757-anti-hsp90-antibody-d7a-western-blot-human-knockout-lysate.jpg)
Western blot - Anti-Hsp90 antibody [D7a] (ab59459)All lanes : Anti-Hsp90 antibody [D7a] (ab59459) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HSP90AA1 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HAP1 whole cell lyate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution
Predicted band size: 95 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab59459 observed at 90 kDa. Red - loading control ab181602 observed at 36 kDa.
ab59459 Anti-Hsp90 antibody [D7a] was shown to specifically react with Hsp90 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266591 (knockout cell lysate ab258458) was used. Wild-type and Hsp90 knockout samples were subjected to SDS-PAGE. ab59459 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 antibody [D7a] (ab59459)](https://www.abcam.cn/ps/products/59/ab59459/Images/Hsp90-alpha-Primary-antibodies-ab59459-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 antibody [D7a] (ab59459)ab59459 staining Hsp90 alpha in human colon cancer tissue section by immunohistochemistry (Bouin's fixed paraffin embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave in citrate buffer. The primary antibody was used at dilution of 1/100,000 dilution using antibody amplifier™ system.
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![Western blot - Anti-Hsp90 antibody [D7a] (ab59459)](https://www.abcam.cn/ps/products/59/ab59459/Images/ab59459-286271-ab59459KOWb.jpg)
Western blot - Anti-Hsp90 antibody [D7a] (ab59459)Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Hsp90 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: Hek 293 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab59459 observed at 90 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab59459 was shown to specifically recognize Hsp90 in wild-type HAP1 cells along with additional cross reactive bands. No band was observed when Hsp90 knockout cells were examined. Wild-type and Hsp90 knockout samples were subjected to SDS-PAGE. Ab59459 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (26)
ab59459 被引用在 26 文献中.
- Tan B et al. Irradiation Induces Gasdermin E-Triggered Tumor Immunity to Inhibit Esophageal Carcinoma Cell Survival. ACS Omega 8:46438-46449 (2023). PubMed: 38107880
- Wu L et al. Effect and Mechanism Analysis of Pig FUT8 Gene on Resistance to Escherichia coli F18 Infection. Int J Mol Sci 23:N/A (2022). PubMed: 36499043
- Elhasnaoui J et al. A Regulatory Axis between Epithelial Splicing Regulatory Proteins and Estrogen Receptor α Modulates the Alternative Transcriptome of Luminal Breast Cancer. Int J Mol Sci 23:N/A (2022). PubMed: 35887187
- Lackie RE et al. Stress-inducible phosphoprotein 1 (HOP/STI1/STIP1) regulates the accumulation and toxicity of α-synuclein in vivo. Acta Neuropathol 144:881-910 (2022). PubMed: 36121476
- Scerri J et al. PKC-mediated phosphorylation and activation of the MEK/ERK pathway as a mechanism of acquired trastuzumab resistance in HER2-positive breast cancer. Front Endocrinol (Lausanne) 13:1010092 (2022). PubMed: 36329884