Anti-Hsp90 alpha抗体[5G5] (ab128483)
![Western blot - Anti-Hsp90 alpha antibody [5G5] (ab128483)](https://www.abcam.cn/ps/products/128/ab128483/Images/ab128483-142231-anti-hsp90-alpha-antibody-5g5-western-blot.jpg "Western blot - Anti-Hsp90 alpha antibody [5G5] (ab128483)")
Key features and details
- Mouse monoclonal [5G5] to Hsp90 alpha
- Suitable for: WB, ELISA, IHC-P, Flow Cyt
- Reacts with: Mouse, Rat, Human, African green monkey
- Isotype: IgG1
概述
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产品名称
Anti-Hsp90 alpha抗体[5G5]
参阅全部 Hsp90 alpha 一抗 -
描述
小鼠单克隆抗体[5G5] to Hsp90 alpha -
宿主
Mouse -
经测试应用
适用于: WB, ELISA, IHC-P, Flow Cytmore details -
种属反应性
与反应: Mouse, Rat, Human, African green monkey -
免疫原
Purified recombinant fragment of Human Hsp90 alpha expressed in E. coli.
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阳性对照
- Human Hsp90 alpha (amino acids 275-484) recombinant protein; NIH 3T3, HeLa, HCT116, HL60, SK-Br-3, C6 and C0S7 cell lysates
Flow Cyt: HeLa cells
IHC-P: Lung cancer tissue
- Human Hsp90 alpha (amino acids 275-484) recombinant protein; NIH 3T3, HeLa, HCT116, HL60, SK-Br-3, C6 and C0S7 cell lysates
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: 99% PBS
Contains 0.5% protein stabilizer. -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
Purified from tissue culture supernatant. -
克隆
单克隆 -
克隆编号
5G5 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab128483于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/2000. Predicted molecular weight: 85 kDa.
|
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| ELISA |
1/10000.
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| IHC-P |
1/200 - 1/1000.
|
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| Flow Cyt |
1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
WB
1/500 - 1/2000. Predicted molecular weight: 85 kDa. |
|
ELISA
1/10000. |
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IHC-P
1/200 - 1/1000. |
|
Flow Cyt
1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Molecular chaperone. Has ATPase activity. -
序列相似性
Belongs to the heat shock protein 90 family. -
翻译后修饰
ISGylated. -
细胞定位
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 3320 Human
- Entrez Gene: 15519 Mouse
- Entrez Gene: 299331 Rat
- Omim: 140571 Human
- SwissProt: P07900 Human
- SwissProt: P07901 Mouse
- SwissProt: P82995 Rat
- Unigene: 525600 Human
see all -
别名
- EL52 antibody
- epididymis luminal secretory protein 52 antibody
- Heat shock 86 kDa antibody
see all
图片
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Western blot - Anti-Hsp90 alpha antibody [5G5] (ab128483)Anti-Hsp90 alpha antibody [5G5] (ab128483) at 1/500 dilution + Human Hsp90 alpha (amino acids 275-484) recombinant protein
Predicted band size: 85 kDa
Expected MWt of recombinant protein is 50.5 kDa. -
![ELISA - Anti-Hsp90 alpha antibody [5G5] (ab128483)](https://www.abcam.cn/ps/products/128/ab128483/Images/ab128483-142232-anti-hsp90-alpha-antibody-5g5-elisa.gif)
ELISA - Anti-Hsp90 alpha antibody [5G5] (ab128483)ELISA using ab128483. -
![Western blot - Anti-Hsp90 alpha antibody [5G5] (ab128483)](https://www.abcam.cn/ps/products/128/ab128483/Images/ab128483-142230-anti-hsp90-alpha-antibody-5g5-western-blot.jpg)
Western blot - Anti-Hsp90 alpha antibody [5G5] (ab128483)All lanes : Anti-Hsp90 alpha antibody [5G5] (ab128483) at 1/500 dilution
Lane 1 : NIH 3T3 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HCT116 cell lysate
Lane 4 : HL-60 cell lysate
Lane 5 : SK-Br-3 cell lysate
Lane 6 : C6 cell lysate
Lane 7 : COS7 cell lysate
Predicted band size: 85 kDa -
![Flow Cytometry - Anti-Hsp90 alpha antibody [5G5] (ab128483)](https://www.abcam.cn/ps/products/128/ab128483/Images/ab128483-142229-anti-hsp90-alpha-antibody-5g5-flow-cytometry.jpg)
Flow Cytometry - Anti-Hsp90 alpha antibody [5G5] (ab128483)ab128483, at a 1/200 dilution, staining Hsp90 alpha in HeLa cells by Flow Cytometry (green). Negative control is shown in red. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 alpha antibody [5G5] (ab128483)](https://www.abcam.cn/ps/products/128/ab128483/Images/ab128483-142228-anti-hsp90-alpha-antibody-5g5-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 alpha antibody [5G5] (ab128483)ab128483, at a 1/200 dilution, staining Hsp90 alpha in paraffin embedded lung cancer tissue by Immunohistochemistry, with DAB staining. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 alpha antibody [5G5] (ab128483)](https://www.abcam.cn/ps/products/128/ab128483/Images/ab128483-142227-anti-hsp90-alpha-antibody-5g5-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 alpha antibody [5G5] (ab128483)ab128483, at a 1/200 dilution, staining Hsp90 alpha in paraffin embedded lung cancer tissue by Immunohistochemistry, with DAB staining.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab128483 被引用在 1 文献中.
- Wang Q & Liu X VDAC upregulation and aTAT1-mediated a-tubulin acetylation contribute to tanespimycin-induced apoptosis in Calu-1 cells. Oncol Rep 44:2725-2734 (2020). PubMed: 33125132