Anti-Hsp70抗体[N27F3-4] (ab47454)
![Immunoprecipitation - Anti-Hsp70 antibody [N27F3-4] (ab47454)](https://www.abcam.cn/ps/products/47/ab47454/Images/ab47454-212668-anti-hsp70-antibody-n27f3-4-immunoprecipitation.jpg "Immunoprecipitation - Anti-Hsp70 antibody [N27F3-4] (ab47454)")
Key features and details
- Mouse monoclonal [N27F3-4] to Hsp70
- Suitable for: IHC-P, WB, IP, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
概述
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产品名称
Anti-Hsp70抗体[N27F3-4]
参阅全部 Hsp70 一抗 -
描述
小鼠单克隆抗体[N27F3-4] to Hsp70 -
宿主
Mouse -
特异性
Detects 72 and 73kDa proteins corresponding to the molecular mass of inducible Hsp and Hsc70 of SDS PAGE immunoblots. -
经测试应用
适用于: IHC-P, WB, IP, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Plants
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免疫原
Recombinant full length protein corresponding to Human Hsp70. Recombinant hsp70/hsc70 (Human)
Database link: P11142, P34932 -
阳性对照
- HeLa nuclear extract lysate (ab14655) can be used as a positive control in WB. ICC/IF: Hek293 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
N27F3-4 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab47454于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/1000.
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| WB | (2) |
1/1000. Predicted molecular weight: 70 kDa.
|
| IP |
Use a concentration of 5 µg/ml.
|
|
| ICC/IF |
1/50.
|
| 说明 |
|---|
|
IHC-P
1/1000. |
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WB
1/1000. Predicted molecular weight: 70 kDa. |
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IP
Use a concentration of 5 µg/ml. |
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ICC/IF
1/50. |
靶标
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相关性
Function: In cooperation with other chaperones, the Hsp70 family stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Tissue specificity: HSPA1B is testis-specific. -
细胞定位
Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. -
数据库链接
- Entrez Gene: 15511 Human
- Entrez Gene: 3303 Human
- Entrez Gene: 193740 Mouse
- Entrez Gene: 3304 Mouse
- Entrez Gene: 24472 Rat
- Entrez Gene: 294254 Rat
- Omim: 140550 Human
- Omim: 603012 Human
see all -
别名
- DnaK type molecular chaperone HSP70 1 antibody
- Epididymis secretory protein Li 103 antibody
- FLJ54303 antibody
see all
图片
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Immunoprecipitation - Anti-Hsp70 antibody [N27F3-4] (ab47454)
Hsp70 was immunoprecipitated using 0.5mg Rat Testis tissue lysate, 5µg of Mouse monoclonal to Hsp70 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Rat Testis tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab47454.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.
Band: 70kDa, non specific bands - 50kDa: We are unsure as to the identity of this extra band; Hsp70
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [N27F3-4] (ab47454)](https://www.abcam.cn/ps/products/47/ab47454/Images/ab47454-316172-anti-hsp70-antibody-n27f3-4-immunohistochemistry.png)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [N27F3-4] (ab47454)Immunohistochemistry analysis using ab47454 (1:100) staining paraffin-embedded mouse backskin. Stained for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.
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![Western blot - Anti-Hsp70 antibody [N27F3-4] (ab47454)](https://www.abcam.cn/ps/products/47/ab47454/Images/ab47454-205117-anti-hsp70-antibody-n27f3-4-western-blot.jpg)
Western blot - Anti-Hsp70 antibody [N27F3-4] (ab47454)This image is courtesy of an anonymous AbreviewAnti-Hsp70 antibody [N27F3-4] (ab47454) at 1/500 dilution + Apteronotus leptorhynchus brain tissue lysate at 50 µg
Secondary
Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal at 1/1000 dilution
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 70 kDa
Exposure time: 5 minutes
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![Western blot - Anti-Hsp70 antibody [N27F3-4] (ab47454)](https://www.abcam.cn/ps/products/47/ab47454/Images/ab47454-59912-anti-hsp70-antibody-n27f3-4-western-blot.jpg)
Western blot - Anti-Hsp70 antibody [N27F3-4] (ab47454)All lanes : Anti-Hsp70 antibody [N27F3-4] (ab47454) at 1 µg/ml
Lane 1 : Cell lysate prepared from human A431 cell line
Lane 2 : Cell lysate prepared from human A549 cell line
Lane 3 : Cell lysate prepared from human HCT116 cell line
Lane 4 : Cell lysate prepared from human Hela cell line
Lane 5 : Cell lysate prepared from human HEK293 cell line
Lane 6 : Cell lysate prepared from human HepG2 cell line
Lane 7 : Cell lysate prepared from human HL-60 cell line
Lane 8 : Cell lysate prepared from human HUVEC cell line
Lane 9 : Cell lysate prepared from human Jurkat cell line
Lane 10 : Cell lysate prepared from human MCF7 cell line
Lane 11 : Cell lysate prepared from human PC3 cell line
Lane 12 : Cell lysate prepared from human T98G cell line
Lane 13 : Lysate prepared from rat brain tissue
Lysates/proteins at 10 µg per lane.
Predicted band size: 70 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Hsp70 antibody [N27F3-4] (ab47454)](https://www.abcam.cn/ps/products/47/ab47454/Images/ab47454-59911-anti-hsp70-antibody-n27f3-4-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Hsp70 antibody [N27F3-4] (ab47454)ICC/IF image of ab47454 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47454, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab47454 被引用在 3 文献中.
- Kuncha SK et al. Genomic innovation of ATD alleviates mistranslation associated with multicellularity in Animalia. Elife 9:N/A (2020). PubMed: 32463355
- Li M et al. Exosomal miR-126 blocks the development of non-small cell lung cancer through the inhibition of ITGA6. Cancer Cell Int 20:574 (2020). PubMed: 33317527
- Zhou JB et al. Marine derived xyloketal derivatives exhibit anti-stress and anti-ageing effects through HSF pathway in Caenorhabditis elegans. Eur J Med Chem 148:63-72 (2018). WB . PubMed: 29454917