Anti-Hsp70抗体[C92F3A-5] (ab47455)
Key features and details
- Mouse monoclonal [C92F3A-5] to Hsp70
- Suitable for: WB, Flow Cyt, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
Anti-Hsp70抗体[C92F3A-5]
参阅全部 Hsp70 一抗 -
描述
小鼠单克隆抗体[C92F3A-5] to Hsp70 -
宿主
Mouse -
特异性
Detects a 70kDa protein corresponding to the molecular mass of Hsp70 of SDS PAGE immunoblots. There is no cross-reactivity to Hsc70 (Hsp73). -
经测试应用
适用于: WB, Flow Cyt, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Cow, Dog, Pig, Caenorhabditis elegans, Macaque monkey, Bos mutus grunniens, Saguinus oedipus, Onchocerca volvulus, Oncorhynchus tshawytscha -
免疫原
Recombinant fragment corresponding to Human Hsp70 aa 436-503.
Sequence:YSDNQPGVLIQVYEGERAMTKDNNLLGRFELSGIPPAPRGVPQIEVTFDI DANGILNVTATDKSTGKANKITI
Database link: P08107 -
表位
The mapped epitope is in the region of amino acid residues 436-503. -
阳性对照
- WB: HeLa, A431, A549, HCT116, HEK293, HepG2, HL-60, HUVEC, Jurkat, MCF7, PC3, T98G cell lysates; Rat Brain tumour lysate; Rat bone lysate. IHC-P: Mouse colon cancer tissue; human colon cancer tissue. Flow cyt: Heat Shocked CD3+ CD8+ T cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.1% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
C92F3A-5 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab47455于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (3) |
1/1000. Predicted molecular weight: 70 kDa.
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Flow Cyt |
1/1000.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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IHC-P |
1/10000.
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说明 |
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WB
1/1000. Predicted molecular weight: 70 kDa. |
Flow Cyt
1/1000. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-P
1/10000. |
靶标
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相关性
Function: In cooperation with other chaperones, the Hsp70 family stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Tissue specificity: HSPA1B is testis-specific. -
细胞定位
Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. -
数据库链接
- Entrez Gene: 281825 Cow
- Entrez Gene: 403612 Dog
- Entrez Gene: 15511 Human
- Entrez Gene: 3303 Human
- Entrez Gene: 193740 Mouse
- Entrez Gene: 3304 Mouse
- Entrez Gene: 396906 Pig
- Entrez Gene: 24472 Rat
see all -
别名
- DnaK type molecular chaperone HSP70 1 antibody
- Epididymis secretory protein Li 103 antibody
- FLJ54303 antibody
see all
图片
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All lanes : Anti-Hsp70 antibody [C92F3A-5] (ab47455) at 1 µg/ml
Lane 1 : Cell lysate prepared from human A431 cell line
Lane 2 : Cell lysate prepared from human A549 cell line
Lane 3 : Cell lysate prepared from human HCT116 cells line
Lane 4 : Cell lysate prepared from human Hela cell line
Lane 5 : Cell lysate prepared from human HEK293 cell line
Lane 6 : Cell lysate prepared from human HepG2 cell line
Lane 7 : Cell lysate prepared from human HL-60 cell line
Lane 8 : Cell lysate prepared from human HUVEC cell line
Lane 9 : Cell lysate prepared from human Jurkat cell line
Lane 10 : Cell lysate prepared from human MCF7 cell line
Lane 11 : Cell lysate prepared from human PC3 cell line
Lane 12 : Cell lysate prepared from human T98G cell line
Lane 13 : Rat brain tissue lysates
Predicted band size: 70 kDaAll are cancer cell lines.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [C92F3A-5] (ab47455)
Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for Hsp70 using ab47455 at 1/1000 dilution in immunohistochemical analysis. Counter stained with hematoxylin.
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All lanes : Anti-Hsp70 antibody [C92F3A-5] (ab47455) at 1/500 dilution
All lanes : Rat bone (tibia) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 70 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [C92F3A-5] (ab47455)
ab47455 staining Hsp70 in mouse colon cancer tissue section by Immunohistochemistry (Bouin's fixed paraffin embedded tissue sections). The primary antibody was diluted at 1/100,000. A Fluorophore conjugated goat anti mouse was used as secondary. An antibody amplifier™ system was used for staining.
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FACS analysis using ab47455 staining heat shock treated CD3 + CD8 + T cells.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (40)
ab47455 被引用在 40 文献中.
- Roboti P et al. Mitochondrial antiviral-signalling protein is a client of the BAG6 protein quality control complex. J Cell Sci 135:N/A (2022). PubMed: 35543156
- Andersson R et al. Differential role of cytosolic Hsp70s in longevity assurance and protein quality control. PLoS Genet 17:e1008951 (2021). PubMed: 33428620
- Andersson S et al. Genome-wide imaging screen uncovers molecular determinants of arsenite-induced protein aggregation and toxicity. J Cell Sci 134:N/A (2021). PubMed: 34085697
- McMahon S et al. DNAJB chaperones suppress destabilised protein aggregation via a region distinct from that used to inhibit amyloidogenesis. J Cell Sci 134:N/A (2021). PubMed: 33674449
- Shi H et al. Regulating glycolysis, the TLR4 signal pathway and expression of RBM3 in mouse liver in response to acute cold exposure. Stress 22:366-376 (2019). PubMed: 30821572