Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-Hsp70抗体[BB70] (ab53496)

Submit a review Q&A (3)References (7)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [BB70] (ab53496)
  • Western blot - Anti-Hsp70 antibody [BB70] (ab53496)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [BB70] (ab53496)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [BB70] (ab53496)
  • Western blot - Anti-Hsp70 antibody [BB70] (ab53496)
  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp70 antibody [BB70] (ab53496)
  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp70 antibody [BB70] (ab53496)
  • Flow Cytometry - Anti-Hsp70 antibody [BB70] (ab53496)

Key features and details

  • Mouse monoclonal [BB70] to Hsp70
  • Suitable for: IHC-P, ICC/IF, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Cow, Human
  • Isotype: IgG2a

概述

  • 产品名称

    Anti-Hsp70抗体[BB70]
    参阅全部 Hsp70 一抗

  • 描述

    小鼠单克隆抗体[BB70] to Hsp70

  • 宿主

    Mouse

  • 特异性

    Detects ~72 (Hsp) and ~73kDa (Hsc).

  • 经测试应用

    适用于: IHC-P, ICC/IF, Flow Cyt, WBmore details

  • 种属反应性

    与反应: Mouse, Rat, Cow, Human

  • 免疫原

    Other Immunogen Type corresponding to Chicken Hsp70.
    Database link: P08106

  • 阳性对照

    • WB: Hela cell lysate; Bovine MDBK cell lysate. IHC-P: Mouse and human colon cancer tissues. Flow Cyt: Jurkat cells.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab53496于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
1/200.
ICC/IF
1/200.
Flow Cyt
Use 0.5µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. 
WB
1/1000. Predicted molecular weight: 70 kDa.

Detects 72kDa and 73kDa proteins corresponding to the molecular mass of inducible hsp and hsc70 on SDS PAGE immunoblots.
说明
IHC-P
1/200.
ICC/IF
1/200.
Flow Cyt
Use 0.5µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. 
WB
1/1000. Predicted molecular weight: 70 kDa.

Detects 72kDa and 73kDa proteins corresponding to the molecular mass of inducible hsp and hsc70 on SDS PAGE immunoblots.

靶标

  • 相关性

    Function: In cooperation with other chaperones, the Hsp70 family stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Tissue specificity: HSPA1B is testis-specific.

  • 细胞定位

    Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

  • 数据库链接

    see all

  • 别名

    • DnaK type molecular chaperone HSP70 1 antibody
    • Epididymis secretory protein Li 103 antibody
    • FLJ54303 antibody
    • FLJ54370 antibody
    • FLJ54392 antibody
    • FLJ54408 antibody
    • FLJ75127 antibody
    • Heat shock 70 kDa protein 1 antibody
    • Heat shock 70 kDa protein 1/2 antibody
    • Heat shock 70 kDa protein 1A antibody
    • Heat shock 70 kDa protein 1A/1B antibody
    • Heat shock 70kDa protein 1B antibody
    • Heat shock induced protein antibody
    • HEL S 103 antibody
    • HSP70 1 antibody
    • HSP70 1A antibody
    • HSP70 1B antibody
    • HSP70 2 antibody
    • HSP70-1 antibody
    • HSP70-1/HSP70-2 antibody
    • HSP70-1A antibody
    • HSP70-2 antibody
    • HSP70.1 antibody
    • HSP70.1/HSP70.2 antibody
    • HSP71_HUMAN antibody
    • HSP72 antibody
    • HSPA1 antibody
    • HSPA1A antibody
    • HSPA1B antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [BB70] (ab53496)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [BB70] (ab53496)

    ab53496 at 100,000 dilution staining Hsp70 + Hsc70 in mouse colon cancer tissue section by immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). A antibody amplifier™ system was used for staining. A HRP-conjugated secondary antibody was used at 1/10 dilution. 

  • Western blot - Anti-Hsp70 antibody [BB70] (ab53496)
    Western blot - Anti-Hsp70 antibody [BB70] (ab53496)
    Anti-Hsp70 antibody [BB70] (ab53496) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

    Predicted band size: 70 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [BB70] (ab53496)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [BB70] (ab53496)

    Ab53496, at a dilution of 1/200, staining Hsp70 + Hsc70 in liver smears of rat hepatocytes by Immunohistochemistry Formalin fixed Parafin embedded tissue.

    1. Control

    2. Two hours after exposure tp hyperthermic stress   

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [BB70] (ab53496)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [BB70] (ab53496)

    ab53496 at 100,000 dilution staining Hsp70 + Hsc70 in human colon cancer tissue section by immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). A antibody amplifier™ system was used for staining.

  • Western blot - Anti-Hsp70 antibody [BB70] (ab53496)
    Western blot - Anti-Hsp70 antibody [BB70] (ab53496)
    Anti-Hsp70 antibody [BB70] (ab53496) at 1/1000 dilution + Bovine MDBK cells

    Predicted band size: 70 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp70 antibody [BB70] (ab53496)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsp70 antibody [BB70] (ab53496)

    Ab53496, at a 1/200 dilution, staining Hsp70 + Hsc70 in nuclear smears of rat hepatocytes by immunocytochemistry. 

    1. Control

    2.Five hours after exposure to hyperthermic stress

       

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp70 antibody [BB70] (ab53496)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsp70 antibody [BB70] (ab53496)Image from Rice WL et al., PLoS One. 2012;7(2):e32843. Epub 2012 Feb 28. Fig 6.; doi:10.1371/journal.pone.0032843; February 28, 2012, PLoS ONE 7(2): e32843.
    ab53496 staining Hsp70 in LLC-PK1 cells expressing Hsp70 under cold block conditions, by Immunocytochemistry/ Immunofluorescence.

    Cells were fixed with 4% paraformaldehyde, permeabilized with 0.01% Triton X-100 in PBS for 4 minutes and blocked with 1% BSA/PBS for 20 minutes. Samples were incubated with primary antibody overnight at 4°C.
  • Flow Cytometry - Anti-Hsp70 antibody [BB70] (ab53496)
    Flow Cytometry - Anti-Hsp70 antibody [BB70] (ab53496)
    Overlay histogram showing Jurkat cells stained with ab53496 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53496, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

文献 (7)

发表研究结果有使用 ab53496？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab53496 被引用在 7 文献中.

  • Zhou C  et al. Gut Microbiota and Serum Metabolite Potential Interactions in Growing Layer Hens Exposed to High-Ambient Temperature. Front Nutr 9:877975 (2022). PubMed: 35571932
  • Sugito S  et al. Immunohistochemical Expression of AQP2 and HSP70 in Broiler Kidney Tissue Treated with Salix tetrasperma Roxb. Extract under Heat Exposure. ScientificWorldJournal 2021:8711286 (2021). PubMed: 34707467
  • Kim JW  et al. Leptin Modulates the Metastasis of Canine Inflammatory Mammary Adenocarcinoma Cells Through Downregulation of Lysosomal Protective Protein Cathepsin A (CTSA). Int J Mol Sci 21:N/A (2020). PubMed: 33255835
  • Ha J  et al. Hydrogen sulfide inhibits Kir2 and Kir3 channels by decreasing sensitivity to the phospholipid phosphatidylinositol 4,5-bisphosphate (PIP2). J Biol Chem 293:3546-3561 (2018). PubMed: 29317494
  • Tang S  et al. Rosemary Reduces Heat Stress by Inducing CRYAB and HSP70 Expression in Broiler Chickens. Oxid Med Cell Longev 2018:7014126 (2018). PubMed: 30425783
  • Xiao B  et al. Endothelial cell-derived exosomes protect SH-SY5Y nerve cells against ischemia/reperfusion injury. Int J Mol Med 40:1201-1209 (2017). PubMed: 28849073
  • Rice WL  et al. Differential, phosphorylation dependent trafficking of AQP2 in LLC-PK1 cells. PLoS One 7:e32843 (2012). ICC/IF ; Human . PubMed: 22403603

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-3 of 3 Abreviews or Q&A

Question

ich würde gerne diese Antikörper einfach so bestellen ohne an diesem
Verfahren teilzunehmen. Ist das für Sie so in Ordnung?
Danke, mit freundlichen Grüßen

Read More
Answer

Vielen Dank für Ihre Antwort.

Die Frau Hoffmann ist außer Haus und ich hoffe es ist Ihnen Recht, dass ich mich um Ihren Fall kümmere.

Unser AbTrial ist nur ein Angebot, welches Sie nicht in Anspruch nehmen müssen. Natürlich können Sie jedes unserer Produkte bestellen dass Sie möchten ohne jegliche weitere Verpflichtung.

Ich hoffe dies hilft Ihnen weiter und ich wünsche Ihnen viel Glück mit Ihren Versuchen.

Read More

Question

Sehr geehrte Damen und Herren,
ich würde gerne folgende Antikörper bestellen, nur hätte ich folgende Frage:
1x ab79275
1x ab53496
Behindern sich diese beiden Antikörper in ihrer Bindestelle, wenn ich
beide im ELISA einsetze.
Danke im Voraus, mit freundlichen Grüßen

Read More
Answer

Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Leider muss ich Ihnen bestätigen, dass ab53496 und ab79275 nicht in ELISA getestet sind und deshalb auch nicht unserer Garantie unterliegen. Wir wissen auch nicht ob beide Klone das gleiche Epitope binden, da die Epitope nicht gemapped wurden.

Vielleicht finden Sie in unserer Auswahl an anti HSP70 Antikörpern, die schon für ELISA getestet sind ein gutes Pärchen.

Für humane Proben würde ich folgendes vorschlagen:

ab47455 als capture Antikörper und ab48515 als Detektionsantikörper. Prinzipiell ist es von Vorteil einen polyklonalen Detektionsantikörper zu verwenden, da die Signalstärke besser ist und auch eine Epitop-Überlappung weniger wahrscheinlich ist.

Diese zwei Antikörper sind noch nicht in einen sandwichELISA getestet worden. Deshalb würde ich Ihnen folgendes Testprogramm vorschlagen: Abtrial

Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen:
https://www.abcam.com/abtrial

Bitte lassen Sie mich wissen, ob Sie gerne daran teilnehmen möchten und mit welchen Antikörpern. Ich habe Ihre Bestellung noch nicht weitergegeben.

Read More

Question

Can you please provide a western blot image showing the doublet bands for Hsp70 and Hsc70?

Read More
Answer

Thank you for contacting us.

The doublets using ab53496are not always well resolved because there is only ˜1kDa between them. The lab was able to provide the attached image showing the doublet on bovine MDBK cells at a 1:1000 dilution. I hope this helps, please let me know if you need any additional information or assistance.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com