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Anti-Hsp60抗体[LK-2] (ab59458)

Reviews (1) Submit a question References (6)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] (ab59458)
  • Western blot - Anti-Hsp60 antibody [LK-2] (ab59458)
  • Western blot - Anti-Hsp60 antibody [LK-2] (ab59458)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] (ab59458)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] (ab59458)

Key features and details

  • Mouse monoclonal [LK-2] to Hsp60
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-Hsp60抗体[LK-2]
    参阅全部 Hsp60 一抗

  • 描述

    小鼠单克隆抗体[LK-2] to Hsp60

  • 宿主

    Mouse

  • 特异性

    Detects ~60kDa.

  • 经测试应用

    适用于: IHC-P, WBmore details

  • 种属反应性

    与反应: Mouse, Human

  • 免疫原

    Human Hsp60 produced through recombinant DNA methods in E.coli

  • 阳性对照

    • HeLa cell lysate

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab59458于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
1/100.
WB (1)
1/40000. Predicted molecular weight: 60 kDa.
说明
IHC-P
1/100.
WB
1/40000. Predicted molecular weight: 60 kDa.

靶标

  • 功能

    Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.

  • 疾病相关

    Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
    Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life.

  • 序列相似性

    Belongs to the chaperonin (HSP60) family.

  • 细胞定位

    Mitochondrion matrix.
  • Information by UniProt

  • 数据库链接

    • 别名

      • 60 kDa chaperonin antibody
      • 60 kDa heat shock protein, mitochondrial antibody
      • CH60_HUMAN antibody
      • Chaperonin 60 antibody
      • Chaperonin, 60-KD antibody
      • CPN60 antibody
      • fa04a05 antibody
      • GROEL antibody
      • heat shock 60kDa protein 1 (chaperonin) antibody
      • Heat shock protein 1 (chaperonin) antibody
      • Heat shock protein 60 antibody
      • Heat shock protein 65 antibody
      • heat shock protein family D (Hsp60) member 1 antibody
      • HLD4 antibody
      • Hsp 60 antibody
      • HSP 65 antibody
      • HSP-60 antibody
      • HSP60 antibody
      • HSP65 antibody
      • HSPD1 antibody
      • HuCHA60 antibody
      • Mitochondrial matrix protein P1 antibody
      • P60 lymphocyte protein antibody
      • short heat shock protein 60 Hsp60s1 antibody
      • SPG13 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] (ab59458)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] (ab59458)

      Immunohistochemistry analysis of  Paraffin Embedded Human kidney tissue using Mouse Anti-HSP60 Antibody, Clone LK2 (SMC-111) at 1/100 dilution.

       

    • Western blot - Anti-Hsp60 antibody [LK-2] (ab59458)
      Western blot - Anti-Hsp60 antibody [LK-2] (ab59458)
      Anti-Hsp60 antibody [LK-2] (ab59458) at 1/1000 dilution + HeLa cell lysate

      Predicted band size: 60 kDa

    • Western blot - Anti-Hsp60 antibody [LK-2] (ab59458)
      Western blot - Anti-Hsp60 antibody [LK-2] (ab59458)This image is courtesy of an anonymous Abreview
      Lanes 1-3 : Anti-Hsp60 antibody [LK-2] (ab59458) at 1/4000 dilution


      Lanes 1-3 : Saccharomyces cerevisiae whole cell lysate at 6900000 cells
      Lane 4 : Ladder

      Secondary
      Lanes 1-2 : HRP-conjugated goat anti-mouse IgG monoclonal at 1/5000 dilution
      Lane 3 : HRP-conjugated goat anti-mouse IgG monoclonal at 1/2000 dilution


      Performed under reducing conditions.

      Predicted band size: 60 kDa
      Observed band size: 60 kDa


      Exposure time: 25 seconds

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] (ab59458)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] (ab59458)

      ab59464 at 100,000 dilution staining Hsp60 alpha in human colon tissue section by Immunohistochemistry (Bouin's fixed paraffin embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave with 2, 5 minutes incubation intervals in citrate buffer. An antibody amplifier™ system was used for staining. A HRP-conjugated anti mouse secondary was used at 1/10 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] (ab59458)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] (ab59458)

      Paraffin-embedded mouse back skin tissue stained for Hsp60 using ab59458 at 1/100 dilution in immunohistochemical analysis. DAB staining.

    文献 (6)

    发表研究结果有使用 ab59458？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab59458 被引用在 6 文献中.

    • Jiang X  et al. Exploiting genetic diversity and gene synthesis to identify superior nitrogenase NifH protein variants to engineer N2-fixation in plants. Commun Biol 4:4 (2021). PubMed: 33398015
    • Senger F  et al. Spatial integration of mechanical forces by a-actinin establishes actin network symmetry. J Cell Sci 132:N/A (2019). PubMed: 31615968
    • Farrugia G  et al. Aspirin impairs acetyl-coenzyme A metabolism in redox-compromised yeast cells. Sci Rep 9:6152 (2019). PubMed: 30992471
    • Schultz MJ  et al. Variable Inhibition of Zika Virus Replication by Different Wolbachia Strains in Mosquito Cell Cultures. J Virol 91:N/A (2017). PubMed: 28446677
    • Mion F  et al. Mast cells control the expansion and differentiation of IL-10-competent B cells. J Immunol 193:4568-79 (2014). PubMed: 25267976
    • Bivi N  et al. Transcriptome and proteome analysis of osteocytes treated with nitrogen-containing bisphosphonates. J Proteome Res 8:1131-42 (2009). PubMed: 19226166

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    Western blot abreview for Anti-Hsp60 antibody [LK-2]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    6.9e+006 cells
    Gel Running Conditions
    Reduced Denaturing (4-12 % Bis-Tris, MOPS running buffer, semi-dry transfer to nitrocellulose)
    Sample
    Saccharomyces cerevisiae Cell lysate - whole cell (W303alpha)
    Specification
    W303alpha
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Apr 25 2014

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