Name: Anti-Hsp60 antibody [EP1006Y] - Loading Control
Brand: RabMAb
SKU: ab45134
Rating: 5 (1 reviews)

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Western blot - Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1006Y] to Hsp60 - Loading Control
Suitable for: IHC-P, WB
Reacts with: Mouse, Rat, Human

Carrier Free

产品名称

Anti-Hsp60抗体[EP1006Y] - Loading Control
参阅全部 Hsp60 一抗
描述

兔单克隆抗体[EP1006Y] to Hsp60 - Loading Control
宿主

Rabbit
经测试应用

适用于: IHC-P, WBmore details
不适用于: Flow Cyt,ICC/IF or IP
种属反应性

与反应: Mouse, Rat, Human
免疫原

Synthetic peptide corresponding to Human Hsp60 aa 400-500 (internal sequence).
阳性对照
- T47D cell lysate, human breast carcinoma tissue.
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

形式

Liquid
存放说明

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EP1006Y
同种型

IgG
研究领域

Alternative Versions
- Anti-Hsp60 antibody [EP1006Y] - BSA and Azide free (ab239835)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- T-47D whole cell lysate (ab14899)
Recombinant Protein
- Recombinant human Hsp60 protein (ab113192)

The Abpromise guarantee

Abpromise™承诺保证使用ab45134于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
IHC-P		1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB	(1)	1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).

说明
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).

应用说明

Is unsuitable for Flow Cyt,ICC/IF or IP.

功能

Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
疾病相关

Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life.
序列相似性

Belongs to the chaperonin (HSP60) family.
细胞定位

Mitochondrion matrix.
Target information above from: UniProt accession P10809 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 3329 Human
- Entrez Gene: 15510 Mouse
- Entrez Gene: 63868 Rat
- Omim: 118190 Human
- SwissProt: P10809 Human
- SwissProt: P63038 Mouse
- SwissProt: P63039 Rat
- Unigene: 595053 Human
- Unigene: 727543 Human
- Unigene: 1777 Mouse
- Unigene: 102058 Rat
see all
别名
- 60 kDa chaperonin antibody
- 60 kDa heat shock protein, mitochondrial antibody
- CH60_HUMAN antibody
- Chaperonin 60 antibody
- Chaperonin, 60-KD antibody
- CPN60 antibody
- fa04a05 antibody
- GROEL antibody
- heat shock 60kDa protein 1 (chaperonin) antibody
- Heat shock protein 1 (chaperonin) antibody
- Heat shock protein 60 antibody
- Heat shock protein 65 antibody
- heat shock protein family D (Hsp60) member 1 antibody
- HLD4 antibody
- Hsp 60 antibody
- HSP 65 antibody
- HSP-60 antibody
- HSP60 antibody
- HSP65 antibody
- HSPD1 antibody
- HuCHA60 antibody
- Mitochondrial matrix protein P1 antibody
- P60 lymphocyte protein antibody
- short heat shock protein 60 Hsp60s1 antibody
- SPG13 antibody
see all

Western blot - Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134)

All lanes : Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134) at 1/20000 dilution

Lane 1 : T47-D (Human mammary gland ductal carcinoma) whole cell lysates
Lane 2 : HeLa (Human cervix adenocarcinoma) whole cell lysates
Lane 3 : 293T (Human embryonic kidney) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 60 kDa

Blocking/dilution buffer: 5% NFDM /TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134)

Immunohistochemistry analysis of paraffin-embedded Human stomach labeling Hsp60 with ab45134 at a 1/200 dilution. Cytoplasmic staining on the glandular epithelium of the Human stomach shown. Secondary antibody ab97051 Goat Anti-Rabbit IgG H&L (HRP) used at a 1/500 dilution. Counter stained with Hematoxylin.

Inset image: negative control obtained using PBS instead of ab45134, secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134)

All lanes : Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134) at 1/50000 dilution

Lane 1 : Human fetal brain lysates
Lane 2 : Human fetal heart lysates
Lane 3 : Human fetal kidney lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 60 kDa

Blocking/dilution buffer: 5% NFDM /TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134)

Immunohistochemistry analysis of paraffin-embedded Human transitional carcinoma of bladder labeling Hsp60 with ab45134 at a 1/200 dilution. Cytoplasmic staining on tumor cells of the Human bladder transitional cell carcinoma shown. Secondary antibody ab97051 Goat Anti-Rabbit IgG H&L (HRP) used at a 1/500 dilution. Counter stained with Hematoxylin.

Inset image: negative control obtained using PBS instead of ab45134, secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney labeling Hsp60 with ab45134 at a 1/200 dilution. Cytoplasmic staining on the epithelium of Mouse kidney tubules shown. Secondary antibody ab97051 Goat Anti-Rabbit IgG H&L (HRP) used at a 1/500 dilution. Counter stained with Hematoxylin.

Inset image: negative control obtained using PBS instead of ab45134, secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134)

All lanes : Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134) at 1/50000 dilution

Lane 1 : Mouse brain lysates
Lane 2 : Mouse heart lysates
Lane 3 : Rat brain lysates
Lane 4 : Rat heart lysates
Lane 5 : Raw264.7 (Mouse abelson murine leukemia virus-induced tumor) whole cell lysates
Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
Lane 7 : NIH/3T3 (Mouse embryo) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 60 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134)

Immunohistochemistry analysis of paraffin-embedded Rat liver labeling Hsp60 with ab45134 at a 1/200 dilution. Cytoplasmic staining on the hepatocytes of the Rat liver shown. Secondary antibody ab97051 Goat Anti-Rabbit IgG H&L (HRP) used at a 1/500 dilution. Counter stained with Hematoxylin.

Inset image: negative control obtained using PBS instead of ab45134, secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

发表研究结果有使用 ab45134？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab45134 被引用在 15 文献中.

Dörner K et al. Genome-wide RNAi screen identifies novel players in human 60S subunit biogenesis including key enzymes of polyamine metabolism. Nucleic Acids Res 50:2872-2888 (2022). PubMed: 35150276
Niikura R et al. Assessments of prolonged effects of desflurane and sevoflurane on motor learning deficits in aged AppNL-G-F/NL-G-F mice. Mol Brain 15:32 (2022). PubMed: 35387663
Li L et al. Multi-omics profiling of collagen-induced arthritis mouse model reveals early metabolic dysregulation via SIRT1 axis. Sci Rep 12:11830 (2022). PubMed: 35821263
Nebie O et al. Human platelet lysate biotherapy for traumatic brain injury: preclinical assessment. Brain 144:3142-3158 (2021). PubMed: 34086871
Kim HJ et al. RORα Enhances Lysosomal Acidification and Autophagic Flux in the Hepatocytes. Hepatol Commun 5:2121-2138 (2021). PubMed: 34558854

View all Publications for this product

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Application

Western blot

Sample

Human Cell lysate - whole cell (Human fibroblast cell and mitochondria fraction)

Gel Running Conditions

Reduced Denaturing

Loading amount

100 µg

Specification

Human fibroblast cell and mitochondria fraction

Blocking step

BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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提交于 Aug 09 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Compatible Secondaries

Isotype control

Positive Controls

Recombinant Protein

应用

The Abpromise guarantee

靶标

功能

疾病相关

序列相似性

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

文献 (15)

客户评价及客户问答

Western blot abreview for Anti-Hsp60 antibody [EP1006Y] - Loading Control