重组Anti-Hsp60抗体[EP1006Y] - Loading Control (ab45134)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1006Y] to Hsp60 - Loading Control
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Hsp60抗体[EP1006Y] - Loading Control
参阅全部 Hsp60 一抗 -
描述
兔单克隆抗体[EP1006Y] to Hsp60 - Loading Control -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human Hsp60 aa 400-500 (internal sequence).
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阳性对照
- T47D cell lysate, human breast carcinoma tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1006Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab45134于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
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说明 |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa). |
靶标
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功能
Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. -
疾病相关
Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life. -
序列相似性
Belongs to the chaperonin (HSP60) family. -
细胞定位
Mitochondrion matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 3329 Human
- Entrez Gene: 15510 Mouse
- Entrez Gene: 63868 Rat
- Omim: 118190 Human
- SwissProt: P10809 Human
- SwissProt: P63038 Mouse
- SwissProt: P63039 Rat
- Unigene: 595053 Human
see all -
别名
- 60 kDa chaperonin antibody
- 60 kDa heat shock protein, mitochondrial antibody
- CH60_HUMAN antibody
see all
图片
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All lanes : Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134) at 1/20000 dilution
Lane 1 : T47-D (Human mammary gland ductal carcinoma) whole cell lysates
Lane 2 : HeLa (Human cervix adenocarcinoma) whole cell lysates
Lane 3 : 293T (Human embryonic kidney) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 60 kDaBlocking/dilution buffer: 5% NFDM /TBST
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134)
Immunohistochemistry analysis of paraffin-embedded Human stomach labeling Hsp60 with ab45134 at a 1/200 dilution. Cytoplasmic staining on the glandular epithelium of the Human stomach shown. Secondary antibody ab97051 Goat Anti-Rabbit IgG H&L (HRP) used at a 1/500 dilution. Counter stained with Hematoxylin.
Inset image: negative control obtained using PBS instead of ab45134, secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134) at 1/50000 dilution
Lane 1 : Human fetal brain lysates
Lane 2 : Human fetal heart lysates
Lane 3 : Human fetal kidney lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 60 kDaBlocking/dilution buffer: 5% NFDM /TBST
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134)
Immunohistochemistry analysis of paraffin-embedded Human transitional carcinoma of bladder labeling Hsp60 with ab45134 at a 1/200 dilution. Cytoplasmic staining on tumor cells of the Human bladder transitional cell carcinoma shown. Secondary antibody ab97051 Goat Anti-Rabbit IgG H&L (HRP) used at a 1/500 dilution. Counter stained with Hematoxylin.
Inset image: negative control obtained using PBS instead of ab45134, secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134)
Immunohistochemistry analysis of paraffin-embedded Mouse kidney labeling Hsp60 with ab45134 at a 1/200 dilution. Cytoplasmic staining on the epithelium of Mouse kidney tubules shown. Secondary antibody ab97051 Goat Anti-Rabbit IgG H&L (HRP) used at a 1/500 dilution. Counter stained with Hematoxylin.
Inset image: negative control obtained using PBS instead of ab45134, secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134) at 1/50000 dilution
Lane 1 : Mouse brain lysates
Lane 2 : Mouse heart lysates
Lane 3 : Rat brain lysates
Lane 4 : Rat heart lysates
Lane 5 : Raw264.7 (Mouse abelson murine leukemia virus-induced tumor) whole cell lysates
Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
Lane 7 : NIH/3T3 (Mouse embryo) whole cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 60 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134)
Immunohistochemistry analysis of paraffin-embedded Rat liver labeling Hsp60 with ab45134 at a 1/200 dilution. Cytoplasmic staining on the hepatocytes of the Rat liver shown. Secondary antibody ab97051 Goat Anti-Rabbit IgG H&L (HRP) used at a 1/500 dilution. Counter stained with Hematoxylin.
Inset image: negative control obtained using PBS instead of ab45134, secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (15)
ab45134 被引用在 15 文献中.
- Dörner K et al. Genome-wide RNAi screen identifies novel players in human 60S subunit biogenesis including key enzymes of polyamine metabolism. Nucleic Acids Res 50:2872-2888 (2022). PubMed: 35150276
- Niikura R et al. Assessments of prolonged effects of desflurane and sevoflurane on motor learning deficits in aged AppNL-G-F/NL-G-F mice. Mol Brain 15:32 (2022). PubMed: 35387663
- Li L et al. Multi-omics profiling of collagen-induced arthritis mouse model reveals early metabolic dysregulation via SIRT1 axis. Sci Rep 12:11830 (2022). PubMed: 35821263
- Nebie O et al. Human platelet lysate biotherapy for traumatic brain injury: preclinical assessment. Brain 144:3142-3158 (2021). PubMed: 34086871
- Kim HJ et al. RORα Enhances Lysosomal Acidification and Autophagic Flux in the Hepatocytes. Hepatol Commun 5:2121-2138 (2021). PubMed: 34558854