Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-Hsp60抗体(ab82520)

Submit a review Q&A (2)References (2)
Western blot - Anti-Hsp60 antibody (ab82520)

    Key features and details

    • Goat polyclonal to Hsp60
    • Suitable for: WB
    • Reacts with: Mouse, Human, Recombinant fragment
    • Isotype: IgG

    选择批间可重复性更高的重组抗体

    Product image
    Anti-Hsp60 antibody [EPR18245-93] (ab190828)
    • 研究可靠 —— 各批次间结果一致且可重复
    • 长期批量供应 —— 采用重组技术，可实现快速生产
    • 首次实验即可成功 —— 经过大量验证确认了特异性
    • 符合伦理标准 —— 产品不含动物成分

    概述

    • 产品名称

      Anti-Hsp60抗体
      参阅全部 Hsp60 一抗

    • 描述

      山羊多克隆抗体to Hsp60

    • 宿主

      Goat

    • 经测试应用

      适用于: WBmore details

    • 种属反应性

      与反应: Mouse, Human, Recombinant fragment

    • 免疫原

      Recombinant human Hsp60 protein

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab82520于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    1/1000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
    说明
    WB
    1/1000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).

    靶标

    • 功能

      Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.

    • 疾病相关

      Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
      Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life.

    • 序列相似性

      Belongs to the chaperonin (HSP60) family.

    • 细胞定位

      Mitochondrion matrix.
    • Information by UniProt

    • 数据库链接

      • 别名

        • 60 kDa chaperonin antibody
        • 60 kDa heat shock protein, mitochondrial antibody
        • CH60_HUMAN antibody
        • Chaperonin 60 antibody
        • Chaperonin, 60-KD antibody
        • CPN60 antibody
        • fa04a05 antibody
        • GROEL antibody
        • heat shock 60kDa protein 1 (chaperonin) antibody
        • Heat shock protein 1 (chaperonin) antibody
        • Heat shock protein 60 antibody
        • Heat shock protein 65 antibody
        • heat shock protein family D (Hsp60) member 1 antibody
        • HLD4 antibody
        • Hsp 60 antibody
        • HSP 65 antibody
        • HSP-60 antibody
        • HSP60 antibody
        • HSP65 antibody
        • HSPD1 antibody
        • HuCHA60 antibody
        • Mitochondrial matrix protein P1 antibody
        • P60 lymphocyte protein antibody
        • short heat shock protein 60 Hsp60s1 antibody
        • SPG13 antibody
        see all

      图片

      • Western blot - Anti-Hsp60 antibody (ab82520)
        Western blot - Anti-Hsp60 antibody (ab82520)
        All lanes : Anti-Hsp60 antibody (ab82520) at 1/1000 dilution

        Lane 1 : Hsp60
        Active Recombinant Protein
        Lane 2 : GroEL
        Active Recombinant Protein
        Lane 3 : Hsp65 Protein
        Lane 4 : HeLa Cell Lysate
        Lane 5 : 3T3 Cell Lysate
        Lane 6 : RK-13 Cell Lysate

        Predicted band size: 61 kDa
        Observed band size: 61 kDa

      文献 (2)

      发表研究结果有使用 ab82520？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab82520 被引用在 2 文献中.

      • Calì T & Brini M Quantification of organelle contact sites by split-GFP-based contact site sensors (SPLICS) in living cells. Nat Protoc 16:5287-5308 (2021). PubMed: 34686857
      • Cieri D  et al. SPLICS: a split green fluorescent protein-based contact site sensor for narrow and wide heterotypic organelle juxtaposition. Cell Death Differ 25:1131-1145 (2018). PubMed: 29229997

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      1-2 of 2 Abreviews or Q&A

      Question

      Sehr geehrte Damen und Herren! Wir sind auf der Suche nach a.) polyclonal Hsp60 AK biotinyliert b.) mycobacterial Hsp65 AK biotinyliert, geeignet für Elisa. Haben Sie diese Produkte in Ihrem Sortiment? Vielen Dank im vorraus.

      Read More
      Answer

      Vielen Dank für Ihre Anfrage. In der Tat haben wir einige polyklonalen HSP60- Antikörper in unserem Katalog verfügbar, unter anderem zum Beispiel ab105853: ein Kaninchen-polyklonaler, der gegen das humane Volllängenprotein gerichtet ist und für Western blot, ELISA und Radioimmunoassay (RIA) geeignet ist. Click here (or use the following: https://www.abcam.com/Biotin-Hsp60-antibody-ab105853.html). Des Weiteren haben wir auch einige Antikörper gegen HSP60 anderer Spezies verfügbar: ab81137: Click here (or use the following: https://www.abcam.com/Legionella-pneumophila-hsp60-antibody-18-ab81137.html). ab82513: Click here (or use the following: https://www.abcam.com/Hsp60-antibody-ab82513.html). ab82520: Click here (or use the following: https://www.abcam.com/Hsp60-antibody-ab82520.html). Die folgenden HSP65- Antikörper sind bei uns erhältlich: ab20045: Click here (or use the following: https://www.abcam.com/Mycobacterium-tuberculosis-Hsp-65-antibody-BDI578-ab20045.html). ab20519: Click here (or use the following: https://www.abcam.com/Mycobacterium-tuberculosis-Hsp-65-antibody-BDI577-ab20519.html). Falls die Antikörper nicht Biotin-konjugiert vorliegen, kann ich Ihnen unsere EasyLink-Kits empfehlen, mit denen Sie jedes Protein/jeden Antikörper mit dem Konjugat Ihrer Wahl koppeln können (s. Anhang oder https://www.abcam.com/EasyLink), wie zum Beispiel: ab102864: Click here (or use the following: https://www.abcam.com/EasyLink-Biotin-type-A-Conjugation-Kit-3-x-100g-Biotin-ab102864.html). ab102867: Click here (or use the following: https://www.abcam.com/Biotin-type-B-Conjugation-Kit-ab102867.html). Wir geben alle getesteten Spezies und Anwendungen auf unseren Produktdatenblättern an und aktualisieren diese sobald wir neue Informationen erhalten. Sollten Sie einen Antikörper gegen das HSP60 oder HSP65 einer anderen Spezies benötigen, bin ich Ihnen gerne bei der weiteren Suche behilflich. Falls Sie einen Antikörper in einer bisher ungetesteten Anwendung oder Spezies testen möchten, kann ich Ihnen zur Zeit ein spezielles Angebot über einen 100%igen Abreview-Rabatt anbieten. Bei diesem Angebot bekommen Sie einen Rabatt für eine zukünftige Bestellung, wenn Sie uns ein Abreview mit dem Testresultat zusenden. Der Rabatt würde gegen eine erneute Bestellung eines weiteren primären Antikörpers von uns verrechnet werden. Weitere Information schicke ich Ihnen auf Anfrage gerne zu. Alle unsere Produkte unterliegen unserer Abcam-Garantie: Falls sich herausstellt, dass der Antikörper nicht so funktioniert, wie auf dem Datenblatt beschrieben und Sie sich innerhalb von sechs Monaten mit Ihrem Problem an uns wenden, werden wir Ihnen gerne Vorschläge zur Optimierung Ihres Protokolls oder gegebenenfalls einen Ersatz oder eine Gutschrift schicken. Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

      Read More

      Question

      Cusotmer kindly contacted us to inquire about the immungen sequence of this product.

      Read More
      Answer

      Thank you for contacting us. I wish to apologize for the delay in getting a response to your inquiry. To show our appreciation for your cooperation and patience with this, I would be happy to offer to you a 20% discount off any Abcam product by using this code:xxxxx The originator of this product did not provide a range for the recombinant protein used in creating this product. However they have stated that this is based from GenBank number M34664. Using this information I did run a BLAST against Saccharomyces cerevisiae. The results of this BLAST show: Identities = 306/537 (57%), Positives = 404/537 (75%), Gaps = 7/537 (1%) Based on this information we do not believe that this product will work in Saccharomyces cerevisiae. I have reviewed the sequences of our other products as well and have found no other HSP60 products which we can suggest for use in Saccharomyces cerevisiae. You may be able to find an appropriate product by searching Biocompare.com. We also offer customer antibody services through our Epitomics group. More information about this may be found at http://www.epitomics.com/services/ or by emailing service@epitomic.com I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Use our products? Submit an Abreview. Earn rewards! https://www.abcam.com/abreviews

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com