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AB278625

重组Anti-Hsp27 (phospho S82)抗体[HSP27S82-CB2]

Anti-Hsp27 (phospho S82) antibody [HSP27S82-CB2]

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Rabbit Recombinant Monoclonal Hsp27 phospho S82 antibody. Suitable for Flow Cyt, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human HSPB1 pS82.

查看别名

HSP27, HSP28, HSPB1, Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, Heat shock protein family B member 1, Stress-responsive protein 27, HSP 27, SRP27

2 Images
Flow Cytometry - Anti-Hsp27 (phospho S82) antibody [HSP27S82-CB2] (AB278625)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Hsp27 (phospho S82) antibody [HSP27S82-CB2] (AB278625)

Peptide blocking flow cytometric analysis of HeLa cells unstained and untreated as negative control (light blue) or stained and untreated (red) or treated with UV+ plus TPA (green) or untreated and blocked with phospho-peptide (black) or UV plus TPA blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or UV plus TPA and blocked with non-phospho peptide (purple) using Phospho-HSP27 (Ser82) antibody ab278625 at 10 µg/mL.

Flow Cytometry - Anti-Hsp27 (phospho S82) antibody [HSP27S82-CB2] (AB278625)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Hsp27 (phospho S82) antibody [HSP27S82-CB2] (AB278625)

Flow cytometric analysis of HeLa cells unstained and untreated cells as as negative control (blue) or untreated (red) or treated with UV and TPA (green) using ab278625 at 1.0 ng/mL.

不同偶联物与剂型 (3)

  • 660 APC

    APC Anti-Hsp27 (phospho S82) antibody [HSP27S82-CB2]

  • 519 FITC

    FITC Anti-Hsp27 (phospho S82) antibody [HSP27S82-CB2]

  • 578 PE

    PE Anti-Hsp27 (phospho S82) antibody [HSP27S82-CB2]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

HSP27S82-CB2

亚型

IgG

轻链类型

kappa

不含载体蛋白

No

反应种属

Human

应用

Flow Cyt, WB

applications

免疫原

Synthetic Peptide within Human HSPB1 pS82. The exact immunogen used to generate this antibody is proprietary information.

P04792

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搭配使用产品

Assay Kits

AB303740

Human Hsp27 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Hsp27 ELISA Kit (AB303740)

  • 0
  • 0
Primary Antibodies

AB109376

Anti-Hsp27 antibody [EPR5477]

20ul selling size|RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-Hsp27 antibody [EPR5477] (AB109376)

  • 5
  • 2
Primary Antibodies

AB46798

Anti-Hsp60 antibody - Mitochondrial Marker

BOND RX™ Validated|Lab Essentials

Western blot - Anti-Hsp60 antibody - Mitochondrial Marker (AB46798)

  • 5
  • 14
Primary Antibodies

AB170099

Anti-p38 alpha/MAPK14 antibody [E229]

20ul selling size|RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-p38 alpha/MAPK14 antibody [E229] (AB170099)

  • 4
  • 5
Primary Antibodies

AB76313

Anti-Hsp27 (phospho S15) antibody [EP2293Y]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 (phospho S15) antibody [EP2293Y] (AB76313)

  • 4
  • 1
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.001-1 µg/mL", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.005 µg/mL", "WB-species-notes": "<p></p>" } } }
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产品详情

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A/G
存储溶液
Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Hsp27 also known as HSPB1 is a small heat shock protein with a molecular weight of approximately 27 kilodaltons. This protein is expressed in various tissues including muscle heart and brain. It functions as a molecular chaperone that stabilizes unfolded proteins preventing their aggregation. Hsp27 undergoes phosphorylation at specific residues which modulates its chaperone activity and interaction with other proteins.
Biological function summary

Hsp27 plays a critical role in cellular stress response by regulating actin cytoskeleton dynamics and inhibiting apoptosis. It forms part of a complex that includes other proteins such as alphaB-crystallin. This complex facilitates the reorganization of proteins under stress conditions enhancing cell survival during oxidative stress or thermal shock. Hsp27 also modulates inflammatory responses and has been shown to affect cell migration.

Pathways

Hsp27 integrates into the apoptosis and inflammation pathways. It interacts with apoptotic machinery such as caspase proteins to protect cells by hindering apoptosome formation. Additionally Hsp27 can engage with pathways involving the nuclear factor-kappa B (NF-kB) impacting inflammatory signaling. CPTC (carboxyl-pyrene-trioctylamine) can modulate these pathways by altering Hsp27 function and interactions.

Hsp27 has connections to neurodegenerative diseases and cancer. In neurodegenerative conditions such as Alzheimer's disease its chaperone activity is thought to protect neurons from misfolded protein aggregates. In cancer Hsp27 supports tumor cell survival and resistance to chemotherapy by interacting with proteins like Akt and p53. These interactions highlight the complex role of Hsp27 in modulating cellular responses in various pathological states.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed : 10383393, PubMed : 20178975). Plays a role in stress resistance and actin organization (PubMed : 19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed : 23728742).
See full target information HSPB1 pS82
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