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AB90537

Anti-Hsp27 (phospho S82)抗体

Anti-Hsp27 (phospho S82) antibody

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(3 Publications)

Rabbit Polyclonal Hsp27 phospho S82 antibody. Suitable for WB, ICC/IF and reacts with Synthetic peptide, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human HSPB1 phospho S82 conjugated to Keyhole Limpet Haemocyanin.

查看别名

HSP27, HSP28, HSPB1, Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, Heat shock protein family B member 1, Stress-responsive protein 27, HSP 27, SRP27

2 Images
Western blot - Anti-Hsp27 (phospho S82) antibody (AB90537)
  • WB

Unknown

Western blot - Anti-Hsp27 (phospho S82) antibody (AB90537)

All lanes:

Western blot - Anti-Hsp27 (phospho S82) antibody (ab90537) at 1/2000 dilution

Lane 1:

phosphorylated Hsp27 protein

Lane 2:

Non-phosphorylated Hsp27 protein

Predicted band size: 23 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 (phospho S82) antibody (AB90537)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 (phospho S82) antibody (AB90537)

ab90537 staining Hsp27 in HeLa cells by Confocal Immunofluorescence (green). Nuclei are stained in blue pseudocolor using DRAQ5.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

WB, ICC/IF

applications

免疫原

Synthetic Peptide within Human HSPB1 phospho S82 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P04792

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Synthetic peptide": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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性能和储存信息

形式
Liquid
纯度
Whole antiserum
存储溶液
Constituents: Whole serum
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Hsp27 also known as HSPB1 is a small heat shock protein with a molecular weight of approximately 27 kilodaltons. This protein is expressed in various tissues including muscle heart and brain. It functions as a molecular chaperone that stabilizes unfolded proteins preventing their aggregation. Hsp27 undergoes phosphorylation at specific residues which modulates its chaperone activity and interaction with other proteins.
Biological function summary

Hsp27 plays a critical role in cellular stress response by regulating actin cytoskeleton dynamics and inhibiting apoptosis. It forms part of a complex that includes other proteins such as alphaB-crystallin. This complex facilitates the reorganization of proteins under stress conditions enhancing cell survival during oxidative stress or thermal shock. Hsp27 also modulates inflammatory responses and has been shown to affect cell migration.

Pathways

Hsp27 integrates into the apoptosis and inflammation pathways. It interacts with apoptotic machinery such as caspase proteins to protect cells by hindering apoptosome formation. Additionally Hsp27 can engage with pathways involving the nuclear factor-kappa B (NF-kB) impacting inflammatory signaling. CPTC (carboxyl-pyrene-trioctylamine) can modulate these pathways by altering Hsp27 function and interactions.

Hsp27 has connections to neurodegenerative diseases and cancer. In neurodegenerative conditions such as Alzheimer's disease its chaperone activity is thought to protect neurons from misfolded protein aggregates. In cancer Hsp27 supports tumor cell survival and resistance to chemotherapy by interacting with proteins like Akt and p53. These interactions highlight the complex role of Hsp27 in modulating cellular responses in various pathological states.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed : 10383393, PubMed : 20178975). Plays a role in stress resistance and actin organization (PubMed : 19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed : 23728742).
See full target information HSPB1 phospho S82
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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of cancer research and clinical oncology 146:1125-1137 PubMed32200459

2020

Significance of unphosphorylated and phosphorylated heat shock protein 27 as a prognostic biomarker in pancreatic ductal adenocarcinoma.

Applications

IHC-P

Species

Human

Richard Drexler,Kim C Wagner,Mirco Küchler,Bernd Feyerabend,Moritz Kleine,Karl J Oldhafer

Molecular medicine reports 12:3787-3794 PubMed26044344

2015

Reduced expression of HSP27 following HAD-B treatment is associated with Her2 downregulation in NIH:OVCAR-3 human ovarian cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kuo Chu Li,Kyun Heo,Nitin Ambade,Min Kyung Kim,Kyung-Hee Kim,Byong Chul Yoo,Hwa-Seung Yoo

Chemistry & biology 22:186-95 PubMed25699602

2015

Phosphomimics destabilize Hsp27 oligomeric assemblies and enhance chaperone activity.

Applications

Unspecified application

Species

Unspecified reactive species

Blagojce Jovcevski,Megan A Kelly,Anthea P Rote,Tracey Berg,Heidi Y Gastall,Justin L P Benesch,J Andrew Aquilina,Heath Ecroyd
View all publications
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详情请参阅我们的条款与条件。
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