Hsp27重组抗体[RM1148]_Hsp27抗体(ab317708)| Abcam中文官网

产品名称

Anti-Hsp27抗体[RM1148] - BSA and Azide free
参阅全部 Hsp27 一抗
描述

兔重组multiclonal [RM1148] to Hsp27 - BSA and Azide free
宿主

Rabbit
经测试应用

适用于: Flow Cyt (Intra), ICC/IF, WB, IHC-P, IPmore details
种属反应性

与反应: Mouse, Rat, Human
免疫原

This product was produced with the following immunogens:
Full length protein. This information is proprietary to Abcam and/or its suppliers.

Full length protein. This information is proprietary to Abcam and/or its suppliers.
阳性对照
- WB: Wild-type HAP1, HSPB1 knockout HAP1 whole cell lysate, HeLa, MCF7, Mouse colon, Mouse heart, Rat heart, Rat colon, Human cerebellum, Human colon, C2C12 and PC-12 lysates. IHC-P: Human stomach, Mouse colon, Rat colon, Human liver, Mouse liver and Rat liver tissues. ICC/IF: HSPB1 KO HAP1, C2C12 and PC-12 cells. Flow Cyt (Intra): parental HAP1 cell. IP: PC-12, C2C12 and HeLa cells.
常规说明
ab317708 is the carrier-free version of ab317707
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C.
存储溶液

pH: 7.2
Constituent: 100% PBS
无载体

是
Concentration information loading...
纯度

Protein A purified
克隆

Recombinant Multiclonal
克隆编号

RM1148
同种型

IgG
研究领域

The Abpromise guarantee

Abpromise™承诺保证使用ab317708于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
Flow Cyt (Intra)		Use at an assay dependent concentration.
ICC/IF		Use at an assay dependent concentration.
WB		Use at an assay dependent concentration. Predicted molecular weight: 23 kDa.
IHC-P		Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. Boil slides seven times for five minutes each in pH 6 citrate buffer.
IP		Use at an assay dependent concentration.

说明
Flow Cyt (Intra) Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 23 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. Boil slides seven times for five minutes each in pH 6 citrate buffer.
IP Use at an assay dependent concentration.

功能

Involved in stress resistance and actin organization.
组织特异性

Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.
疾病相关

Defects in HSPB1 are the cause of Charcot-Marie-Tooth disease type 2F (CMT2F) [MIM:606595]. CMT2F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. CMT2F onset is between 15 and 25 years with muscle weakness and atrophy usually beginning in feet and legs (peroneal distribution). Upper limb involvement occurs later. CMT2F inheritance is autosomal dominant.
Defects in HSPB1 are a cause of distal hereditary motor neuronopathy type 2B (HMN2B) [MIM:608634]. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective impairment of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs.
序列相似性

Belongs to the small heat shock protein (HSP20) family.
翻译后修饰

Phosphorylated in MCF-7 cells on exposure to protein kinase C activators and heat shock.
细胞定位

Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
Target information above from: UniProt accession P04792 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 3315 Human
- Entrez Gene: 15507 Mouse
- Entrez Gene: 24471 Rat
- Omim: 602195 Human
- SwissProt: P04792 Human
- SwissProt: P14602 Mouse
- SwissProt: P42930 Rat
- Unigene: 520973 Human
- Unigene: 13849 Mouse
- Unigene: 3841 Rat
see all
别名
- Heat shock 27kDa protein antibody
- 28 kDa heat shock protein antibody
- CMT2F antibody
- DKFZp586P1322 antibody
- epididymis secretory protein Li 102 antibody
- Estrogen regulated 24 kDa protein antibody
- Estrogen-regulated 24 kDa protein antibody
- Heat shock 25kDa protein 1 antibody
- Heat shock 27 kDa protein antibody
- Heat shock 27kD protein 1 antibody
- Heat shock 27kDa protein 1 antibody
- Heat shock 28kDa protein 1 antibody
- Heat Shock Protein 27 antibody
- Heat shock protein beta 1 antibody
- Heat shock protein beta-1 antibody
- heat shock protein family B (small) member 1 antibody
- HEL-S-102 antibody
- HMN2B antibody
- HS.76067 antibody
- Hsp 25 antibody
- HSP 27 antibody
- Hsp 28 antibody
- Hsp B1 antibody
- Hsp25 antibody
- HSP27 antibody
- Hsp28 antibody
- HspB1 antibody
- HSPB1_HUMAN antibody
- SRP27 antibody
- Stress responsive protein 27 antibody
- Stress-responsive protein 27 antibody
see all

Western blot - Anti-Hsp27 antibody [RM1148] - BSA and Azide free (ab317708)

All lanes : Anti-Hsp27 antibody [RM1148] (ab317707) at 1/1000 dilution

Lane 1 : Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell) whole cell lysate
Lane 2 : HSPB1 knockout HAP1 whole cell lysate
Lane 3 : HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (800CW) and Goat Anti-Mouse IgG H&L (680RD) at 1/20000 dilution

Predicted band size: 23 kDa
Observed band size: 23-27 kDa why is the actual band size different from the predicted?

Exposure time: 180 seconds

This data was developed using ab317707, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of TBS.

The samples were run on a Bis-Tris gel under reducing conditions.

Western blot: Anti-Hsp27 antibody (ab317707) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5](ab8245) loading control staining at 1/20000 dilution, shown in red.

In Western blot, ab317707 was shown to bind specifically to Hsp27. Target of interest was observed at 23-27 kDa in wild-type HAP1 cell lysates (lane 1) with no signal observed at this size in Hsp27 knockout cell line (lane 2). To generate this image, samples were first run on an SDS-PAGE gel then transferred onto an immobilon-FL PVDF membrane. Membranes were blocked in a fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4°C. Blots were washed in TBS-T, incubated with secondary antibodies Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution for 1 h at room temperature, washed again then imaged.

The expression molecular weight observed is consistent with what has been described in the literature (PMID: 36690850 and PMID: 26675522).
Western blot - Anti-Hsp27 antibody [RM1148] - BSA and Azide free (ab317708)

All lanes : Anti-Hsp27 antibody [RM1148] (ab317707) at 1/1000 dilution

Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lane 3 : Mouse colon tissue lysate
Lane 4 : Mouse heart tissue lysate
Lane 5 : Rat heart tissue lysate
Lane 6 : Rat colon tissue lysate
Lane 7 : Human cerebellum tissue lysate
Lane 8 : Human colon tissue lysate
Lane 9 : C2C12 (mouse myoblast) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 23 kDa
Observed band size: 23-27 kDa why is the actual band size different from the predicted?

Exposure time: 37 seconds

This data was developed using ab317707, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Low expression: brain, cerebellum.

The expression molecular weight observed is consistent with what has been described in the literature (PMID: 36690850 and PMID: 26675522).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
Western blot - Anti-Hsp27 antibody [RM1148] - BSA and Azide free (ab317708)

Anti-Hsp27 antibody [RM1148] (ab317707) at 1/1000 dilution + PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 23 kDa
Observed band size: 23 kDa

Exposure time: 81 seconds

This data was developed using ab317707, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [RM1148] - BSA and Azide free (ab317708)

This data was developed using ab317707, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling Hsp27 with ab317707 at 1/2000 (0.243 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on smooth muscle in human stomach. The section was incubated with ab317707 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [RM1148] - BSA and Azide free (ab317708)

This data was developed using ab317707, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Hsp27 with ab317707 at 1/4000 (0.121 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on smooth muscle in mouse colon. The section was incubated with ab317707 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [RM1148] - BSA and Azide free (ab317708)

This data was developed using ab317707, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Hsp27 with ab317707 at 1/4000 (0.121 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on smooth muscle in rat colon. The section was incubated with ab317707 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [RM1148] - BSA and Azide free (ab317708)

This data was developed using ab317707, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Hsp27 with ab317707 at 1/2000 (0.243 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression: no staining on human liver. The section was incubated with ab317707 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [RM1148] - BSA and Azide free (ab317708)

This data was developed using ab317707, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Hsp27 with ab317707 at 1/4000 (0.121 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression: no staining on mouse liver. The section was incubated with ab317707 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [RM1148] - BSA and Azide free (ab317708)

This data was developed using ab317707, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Hsp27 with ab317707 at 1/4000 (0.121 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression: weak staining on vascular smooth muscle in rat liver. The section was incubated with ab317707 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 antibody [RM1148] - BSA and Azide free (ab317708)

This data was developed using ab317707, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HSPB1 KO HAP1 (HSPB1 knockout human chronic myelogenous leukemia near-haploid cell) cells labelling Hsp27 with ab317707 at 1/500 (0.97 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image displaying cytoplasmic staining in wildtype HAP1 cells, and no staining in HSPB1 knockout HAP1 cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 antibody [RM1148] - BSA and Azide free (ab317708)

This data was developed using ab317707, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized C2C12 (mouse myoblast) cells labelling Hsp27 with ab317707 at 1/500 (0.97 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing cytoplasmic staining in C2C12 cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 antibody [RM1148] - BSA and Azide free (ab317708)

This data was developed using ab317707, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PC-12 (rat adrenal gland pheochromocytoma cell) cells labelling Hsp27 with ab317707 at 1/500 (0.97 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing cytoplasmic staining in PC-12 cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
Flow Cytometry (Intracellular) - Anti-Hsp27 antibody [RM1148] - BSA and Azide free (ab317708)

This data was developed using ab317707, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized parental HAP1(human chronic myelogenous leukemia near-haploid cell, Left) / HSPB1 knockout HAP1(Right) cells labelling Hsp27 with ab317707 at 1/5000 dilution (0.01 ug)/Red (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-Hsp27 antibody [RM1148] - BSA and Azide free (ab317708)

This data was developed using ab317707, the same antibody clone in a different buffer formulation.
Hsp27 was immunoprecipitated from 0.35 mg PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate with ab317707 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317707 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate
Lane 2: ab317707 IP in PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab317707 in PC-12 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
Immunoprecipitation - Anti-Hsp27 antibody [RM1148] - BSA and Azide free (ab317708)

This data was developed using ab317707, the same antibody clone in a different buffer formulation.
Hsp27 was immunoprecipitated from 0.35 mg C2C12 (mouse myoblast) whole cell lysate with ab317707 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317707 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: C2C12 (mouse myoblast) whole cell lysate
Lane 2: ab317707 IP in C2C12 (mouse myoblast) whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab317707 in C2C12 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
Immunoprecipitation - Anti-Hsp27 antibody [RM1148] - BSA and Azide free (ab317708)

This data was developed using ab317707, the same antibody clone in a different buffer formulation.
Hsp27 was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab317707 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317707 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 2: ab317707 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab317707 in HeLa whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
Anti-Hsp27 antibody [RM1148] - BSA and Azide free (ab317708)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheet download

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概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

无载体

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Compatible Secondaries

Related Products

应用

The Abpromise guarantee

靶标

功能

组织特异性

疾病相关

序列相似性

翻译后修饰

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

Datasheet download

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