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AB2790

Anti-Hsp27 抗体 [G3.1]

Anti-Hsp27 antibody [G3.1]

5

(6 Reviews)

|

(49 Publications)

Mouse Recombinant Monoclonal Hsp27 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 49 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human HSPB1.

查看别名

HSP27, HSP28, HSPB1, Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, Heat shock protein family B member 1, Stress-responsive protein 27, HSP 27, SRP27

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 antibody [G3.1] (AB2790)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 antibody [G3.1] (AB2790)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling Hsp27 with ab2790 at 1/100 dilution, followed by AlexaFluor® 488-conjugated Goat anti-Mouse secondary antibody (ab150113) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab9509 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [G3.1] (AB2790)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [G3.1] (AB2790)

Immunohistochemical analysis of paraffin-embedded human tissues labeling Hsp27 with ab2790 at 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). The section was incubated with ab2790 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

Western blot - Anti-Hsp27 antibody [G3.1] (AB2790)
  • WB

Lab

Western blot - Anti-Hsp27 antibody [G3.1] (AB2790)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Hsp27 antibody [G3.1] (ab2790) at 1/1000 dilution

Lane 1:

Human liver tissue lysate at 20 µg

Lane 2:

Human heart tissue lysate at 20 µg

Lane 3:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

549 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg

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关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

G3.1

亚型

IgG1

不含载体蛋白

No

反应种属

Human

应用

WB, IHC-P, ICC/IF

applications

免疫原

Native Full Length Protein corresponding to Human HSPB1.

P04792

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }
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为什么推荐这个？

我们推荐这个产品，因为它经常被用于相同的实验或相关的研究。

我们建议您务必查阅产品说明书，以确认其是否符合您的实验需求；若有疑问，也可联系我们的技术团队获取协助。

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产品详情

This product has switched from a hybridoma to recombinant production method on 1st November 2024.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Hsp27 also known as HSPB1 is a small heat shock protein with a molecular weight of approximately 27 kilodaltons. This protein is expressed in various tissues including muscle heart and brain. It functions as a molecular chaperone that stabilizes unfolded proteins preventing their aggregation. Hsp27 undergoes phosphorylation at specific residues which modulates its chaperone activity and interaction with other proteins.
Biological function summary

Hsp27 plays a critical role in cellular stress response by regulating actin cytoskeleton dynamics and inhibiting apoptosis. It forms part of a complex that includes other proteins such as alphaB-crystallin. This complex facilitates the reorganization of proteins under stress conditions enhancing cell survival during oxidative stress or thermal shock. Hsp27 also modulates inflammatory responses and has been shown to affect cell migration.

Pathways

Hsp27 integrates into the apoptosis and inflammation pathways. It interacts with apoptotic machinery such as caspase proteins to protect cells by hindering apoptosome formation. Additionally Hsp27 can engage with pathways involving the nuclear factor-kappa B (NF-kB) impacting inflammatory signaling. CPTC (carboxyl-pyrene-trioctylamine) can modulate these pathways by altering Hsp27 function and interactions.

Hsp27 has connections to neurodegenerative diseases and cancer. In neurodegenerative conditions such as Alzheimer's disease its chaperone activity is thought to protect neurons from misfolded protein aggregates. In cancer Hsp27 supports tumor cell survival and resistance to chemotherapy by interacting with proteins like Akt and p53. These interactions highlight the complex role of Hsp27 in modulating cellular responses in various pathological states.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed : 10383393, PubMed : 20178975). Plays a role in stress resistance and actin organization (PubMed : 19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed : 23728742).
See full target information HSPB1
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文献 (49)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37834400

2023

Diaphragm Fatigue in SMNΔ7 Mice and Its Molecular Determinants: An Underestimated Issue.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Cadile,Deborah Recchia,Massimiliano Ansaldo,Paola Rossi,Giorgia Rastelli,Simona Boncompagni,Lorenza Brocca,Maria Antonietta Pellegrino,Monica Canepari

Nature communications 14:4092 PubMed37433777

2023

Immunoproteasome-specific subunit PSMB9 induction is required to regulate cellular proteostasis upon mitochondrial dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Minji Kim,Remigiusz A Serwa,Lukasz Samluk,Ida Suppanz,Agata Kodroń,Tomasz M Stępkowski,Praveenraj Elancheliyan,Biniyam Tsegaye,Silke Oeljeklaus,Michal Wasilewski,Bettina Warscheid,Agnieszka Chacinska

The Journal of biological chemistry 299:103019 PubMed36791913

2023

Saturated fatty acid- and/or monounsaturated fatty acid-containing phosphatidic acids selectively interact with heat shock protein 27.

Applications

Unspecified application

Species

Unspecified reactive species

Naoto Yachida,Fumi Hoshino,Chiaki Murakami,Masayuki Ebina,Yuri Miura,Fumio Sakane

Journal of animal science 100: PubMed35908787

2022

The impact of Zearalenone on heat-stressed skeletal muscle in pigs.

Applications

Unspecified application

Species

Unspecified reactive species

Tori E Rudolph,Crystal M Roach,Lance H Baumgard,Jason W Ross,Aileen F Keating,Josh T Selsby

Forensic sciences research 7:247-254 PubMed35784415

2022

Histological examination of carotid artery tissue in cases of ligature strangulation and hanging.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Ulbricht,Burkhard Madea,Elke Doberentz

Frontiers in veterinary science 9:686902 PubMed35300220

2022

Heat Stress-Induced Dysbiosis of Porcine Colon Microbiota Plays a Role in Intestinal Damage: A Fecal Microbiota Profile.

Applications

Unspecified application

Species

Unspecified reactive species

Canying Hu,Yadnyavalkya Patil,Dongliang Gong,Tianyue Yu,Junyu Li,Lianyun Wu,Xiaoxi Liu,Zhichao Yu,Xinbing Ma,Yanhong Yong,Jinjun Chen,Ravi Gooneratne,Xianghong Ju

Journal of extracellular vesicles 10:12084 PubMed33936566

2021

Extracellular vesicles from monocyte/platelet aggregates modulate human atherosclerotic plaque reactivity.

Applications

Unspecified application

Species

Unspecified reactive species

Silvia Oggero,Monica de Gaetano,Simone Marcone,Stephen Fitzsimons,Andreia L Pinto,Dinara Ikramova,Mary Barry,David Burke,Trinidad Montero-Melendez,Dianne Cooper,Thomas Burgoyne,Orina Belton,Lucy V Norling,Eoin P Brennan,Catherine Godson,Mauro Perretti

PloS one 16:e0246050 PubMed33524056

2021

Pseudomonas aeruginosa induces p38MAP kinase-dependent IL-6 and CXCL8 release from bronchial epithelial cells via a Syk kinase pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew S Coates,Eric W F W Alton,Garth W Rapeport,Jane C Davies,Kazuhiro Ito

Human molecular genetics 29:2076-2097 PubMed32420594

2020

The Tudor-domain protein TDRD7, mutated in congenital cataract, controls the heat shock protein HSPB1 (HSP27) and lens fiber cell morphology.

Applications

Unspecified application

Species

Unspecified reactive species

Carrie E Barnum,Salma Al Saai,Shaili D Patel,Catherine Cheng,Deepti Anand,Xiaolu Xu,Soma Dash,Archana D Siddam,Lisa Glazewski,Emily Paglione,Shawn W Polson,Shinichiro Chuma,Robert W Mason,Shuo Wei,Mona Batish,Velia M Fowler,Salil A Lachke

Aging 12:7282-7298 PubMed32315283

2020

HMGN5 promotes IL-6-induced epithelial-mesenchymal transition of bladder cancer by interacting with Hsp27.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Yao,Leye He,Yu Gan,Jianye Liu,Jin Tang,Zhi Long,Jing Tan
View all publications
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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。
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