Anti-Hsp22/HSPB8抗体(ab79784)
Key features and details
- Rabbit polyclonal to Hsp22/HSPB8
- Suitable for: IHC-P, WB, ICC
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-Hsp22/HSPB8抗体
参阅全部 Hsp22/HSPB8 一抗 -
描述
兔多克隆抗体to Hsp22/HSPB8 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICCmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Full length native protein (purified) corresponding to Human Hsp22/HSPB8.
Database link: Q9UJY1 -
阳性对照
- WB: Rat skeletal tissue. IHC-P: Mouse spinal cord and epithelial tissue. ICC: Human HaCaT cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab79784于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/100.
|
|
| WB |
1/1000. Predicted molecular weight: 22 kDa.
|
|
| ICC |
1/100.
|
| 说明 |
|---|
|
IHC-P
1/100. |
|
WB
1/1000. Predicted molecular weight: 22 kDa. |
|
ICC
1/100. |
靶标
-
功能
Displays temperature-dependent chaperone activity. -
组织特异性
Predominantly expressed in skeletal muscle and heart. -
疾病相关
Neuronopathy, distal hereditary motor, 2A
Charcot-Marie-Tooth disease 2L -
序列相似性
Belongs to the small heat shock protein (HSP20) family. -
细胞定位
Cytoplasm. Nucleus. Translocates to nuclear foci during heat shock. - Information by UniProt
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数据库链接
- Entrez Gene: 26353 Human
- Entrez Gene: 80888 Mouse
- Entrez Gene: 113906 Rat
- Omim: 608014 Human
- SwissProt: Q9UJY1 Human
- SwissProt: Q9JK92 Mouse
- SwissProt: Q9EPX0 Rat
- Unigene: 400095 Human
see all -
别名
- Alpha crystallin C chain antibody
- Alpha-crystallin C chain antibody
- Charcot Marie Tooth disease axonal type 2L antibody
see all
图片
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Western blot - Anti-Hsp22/HSPB8 antibody (ab79784)Anti-Hsp22/HSPB8 antibody (ab79784) at 20 µg + Rat skeletal muscle lysate
Predicted band size: 22 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp22/HSPB8 antibody (ab79784)ab79784 at 1/100 dilution staining by Hsp22/HSPB8 in mouse spinal cord tissue section by Immunohistochemistry (Bouin's fixative fixed paraffin-embedded tissue section). Antigen retrieval was done by microwave in citrate buffer. A Fluorophore-conjugated goat anti-rabbit secondary was used at 1/10 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp22/HSPB8 antibody (ab79784)Immunohistochemical analysis of Bouin's fixed paraffin-embedded mouse epithelial tissue using ab79784 at 1/100 dilution for 1 hour at RT, followed by secondary Goat anti-rabbit (green) at 1/50 diltuon for 1 hour at RT.
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Immunocytochemistry - Anti-Hsp22/HSPB8 antibody (ab79784)Immunocytochemical analysis of human HaCaT cells fixed with 100% cold methanol at -20°C for 10 minutes using ab79784 at 1/100 dilution for 12 hours at 4°C, followed by secondary Goat anti-rabbit (green) at 1/50 dilution for 1-2 hours at RT in the dark.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab79784 被引用在 4 文献中.
- Johari M et al. Missense mutations in small muscle protein X-linked (SMPX) cause distal myopathy with protein inclusions. Acta Neuropathol 142:375-393 (2021). PubMed: 33974137
- Yao J et al. The Homeobox gene, HOXB13, Regulates a Mitotic Protein-Kinase Interaction Network in Metastatic Prostate Cancers. Sci Rep 9:9715 (2019). PubMed: 31273254
- Bouhy D et al. A knock-in/knock-out mouse model of HSPB8-associated distal hereditary motor neuropathy and myopathy reveals toxic gain-of-function of mutant Hspb8. Acta Neuropathol 135:131-148 (2018). IHC . PubMed: 28780615
- Ghaoui R et al. Mutations in HSPB8 causing a new phenotype of distal myopathy and motor neuropathy. Neurology 86:391-8 (2016). PubMed: 26718575