Anti-HSP17.6抗体(ab80183)
Key features and details
- Rabbit polyclonal to HSP17.6
- Suitable for: WB
- Reacts with: Arabidopsis thaliana, Recombinant fragment
- Isotype: IgG
概述
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产品名称
Anti-HSP17.6抗体 -
描述
兔多克隆抗体to HSP17.6 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Arabidopsis thaliana, Recombinant fragment -
免疫原
Full length recombinant HSP17.6 protein produced in E. coli and purified by conventional methods (no affinity tag)
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阳性对照
- Heat shocked (38° C for 2 hours) Arabidopsis thaliana lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
Constituent: Whole serum -
Concentration information loading... -
纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab80183于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
(ECL) |
| 说明 |
|---|
|
WB
1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa). (ECL) |
靶标
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相关性
HSP17.6 belongs to the class I family of small heat shock proteins. Small Hsps are induced when plant cells are stressed by increased temperature and appear to be involved in chaperone functions by protecting other proteins from irreversible denaturation. They are produced late in the seed maturation process. -
细胞定位
Cytoplasmic -
数据库链接
- Entrez Gene: 831076 Arabidopsis thaliana
- SwissProt: P13853 Arabidopsis thaliana
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别名
- 17.6 kDa class I heat shock protein antibody
- Arabidopsis thaliana heat shock protein 17.6A antibody
- AT HSP17.6A antibody
see all
图片
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Western blot - Anti-HSP17.6 antibody (ab80183)Lanes 1-2 & 4-7 : Anti-HSP17.6 antibody (ab80183) at 1/1000 dilution
Lane 1 : Total protein from heat shocked Arabidopsis thaliana at 15 µg
Lane 2 : Arabidopsis thaliana control plant
Lane 3 : Empty lane
Lane 4 : Recombinant purified HSP17.6, 1 ng
Lane 5 : Recombinant purified HSP17.6, 2 ng
Lane 6 : Lysates from Arabidopsis thaliana, 5 ng
Lane 7 : recombinant purified HSP17.6, 10 ng
Secondary
Lanes 1-2 & 4-7 : HRP-conjugated goat anti rabbit IgG
Predicted band size: 18 kDa15% SDS-PAGE and blotted 1hour onto nitrocellulose
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab80183 被引用在 4 文献中.
- Yamaguchi N et al. H3K27me3 demethylases alter HSP22 and HSP17.6C expression in response to recurring heat in Arabidopsis. Nat Commun 12:3480 (2021). PubMed: 34108473
- Sedaghatmehr M et al. A regulatory role of autophagy for resetting the memory of heat stress in plants. Plant Cell Environ 42:1054-1064 (2019). PubMed: 30136402
- Davies M et al. Stress in native grasses under ecologically relevant heat waves. PLoS One 13:e0204906 (2018). PubMed: 30308074
- Weng M et al. Histone chaperone ASF1 is involved in gene transcription activation in response to heat stress in Arabidopsis thaliana. Plant Cell Environ 37:2128-38 (2014). PubMed: 24548003