Name: Anti-Hsp105/HSP110 antibody [EPR4576]
Brand: RabMAb
SKU: ab109624
Rating: 4 (4 reviews)

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Western blot - Anti-Hsp105/HSP110 antibody [EPR4576] (ab109624)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4576] to Hsp105/HSP110
Suitable for: WB, IP, IHC-P, ICC/IF
Reacts with: Mouse, Rat, Human

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 APC Carrier Free PE

产品名称

Anti-Hsp105/HSP110抗体[EPR4576]
参阅全部 Hsp105/HSP110 一抗
描述

兔单克隆抗体[EPR4576] to Hsp105/HSP110
宿主

Rabbit
经测试应用

适用于: WB, IP, IHC-P, ICC/IFmore details
种属反应性

与反应: Mouse, Rat, Human
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
阳性对照
- WB: HepG2, MCF-7, HeLa, C6, PC-12 and NIH/3T3 cell lysates and human cerebellum tissue lysate. ICC/IF: HeLa cells. IHC-P: Human thyroid gland carcinoma and liver carcinoma tissues. IP: NIH/3T3 cell lysate.
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
存储溶液

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EPR4576
同种型

IgG
研究领域

The Abpromise guarantee

Abpromise™承诺保证使用ab109624于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
WB	(2)	1/1000 - 1/10000. Predicted molecular weight: 97 kDa.
IP	(1)	1/10 - 1/100.
IHC-P	(1)	1/50 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols.
ICC/IF		1/100 - 1/250.

说明
WB 1/1000 - 1/10000. Predicted molecular weight: 97 kDa.
IP 1/10 - 1/100.
IHC-P 1/50 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols.
ICC/IF 1/100 - 1/250.

功能

Prevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. Inhibits HSPA8/HSC70 ATPase and chaperone activities.
组织特异性

Highly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells.
序列相似性

Belongs to the heat shock protein 70 family.
翻译后修饰

Phosphorylation on Ser-509 may be important for regulation of the HSPA8/HSC70 chaperone activity.
细胞定位

Cytoplasm.
Target information above from: UniProt accession Q92598 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 10808 Human
- Entrez Gene: 15505 Mouse
- Entrez Gene: 288444 Rat
- Omim: 610703 Human
- SwissProt: Q92598 Human
- SwissProt: Q61699 Mouse
- SwissProt: Q66HA8 Rat
- Unigene: 36927 Human
- Unigene: 270681 Mouse
- Unigene: 37805 Rat
see all
别名
- Antigen NY CO 25 antibody
- Antigen NY-CO-25 antibody
- DKFZp686M05240 antibody
- Heat shock 105kD alpha antibody
- Heat shock 105kD antibody
- Heat shock 105kD beta antibody
- Heat shock 105kDa protein 1 antibody
- Heat shock 105kDa protein antibody
- Heat shock 105kDa/110kDa protein 1 antibody
- Heat shock 110 kDa protein antibody
- Heat shock 110kDa protein antibody
- Heat shock protein 105 kDa antibody
- HS105_HUMAN antibody
- HSP105 antibody
- HSP105A antibody
- HSP105B antibody
- HSP110 antibody
- HSPH 1 antibody
- Hsph1 antibody
- KIAA0201 antibody
- NY CO 25 antibody
see all

Western blot - Anti-Hsp105/HSP110 antibody [EPR4576] (ab109624)

All lanes : Anti-Hsp105/HSP110 antibody [EPR4576] (ab109624) at 1/5000 dilution (purified)

Lane 1 : HeLa whole cell lysate
Lane 2 : HepG2 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 97 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Hsp105/HSP110 antibody [EPR4576] (ab109624)

All lanes : Anti-Hsp105/HSP110 antibody [EPR4576] (ab109624) at 1/5000 dilution (purified)

Lane 1 : C6 whole cell lysate
Lane 2 : PC-12 whole cell lysate
Lane 3 : NIH/3T3 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 97 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp105/HSP110 antibody [EPR4576] (ab109624)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver carcinoma tissue labelling Hsp105/HSP110 with purified ab109624 at a dilution of 1/500. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunocytochemistry/ Immunofluorescence - Anti-Hsp105/HSP110 antibody [EPR4576] (ab109624)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling Hsp105/HSP110 with purified ab109624 at a dilution of 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

Control 1: primary antibody (1/250) and secondary antibody, ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000).

Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000).
Immunoprecipitation - Anti-Hsp105/HSP110 antibody [EPR4576] (ab109624)

ab109624 (purified) at 1/40 immunoprecipitating Hsp105/HSP110 in NIH/3T3 whole cell lysate.

Lane 1 (input): NIH/3T3 whole cell lysate (10µg)

Lane 2 (+): ab109624 + NIH/3T3 whole cell lysate.

Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109624 in NIH/3T3 whole cell lysate.

For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Blocking buffer and concentration: 5% NFDM/TBST.

Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-Hsp105/HSP110 antibody [EPR4576] (ab109624)

All lanes : Anti-Hsp105/HSP110 antibody [EPR4576] (ab109624) at 1/1000 dilution (unpurified)

Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Human cerebellum lysate
Lane 4 : MCF-7 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 97 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Hsp105/HSP110 antibody [EPR4576] (ab109624)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling Hsp105/HSP110 with unpurified ab109624 at a dilution of 1/100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp105/HSP110 antibody [EPR4576] (ab109624)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid gland carcinoma tissue labelling Hsp105/HSP110 with unpurified ab109624 at a dilution of 1/50.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-Hsp105/HSP110 antibody [EPR4576] (ab109624)

Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

发表研究结果有使用 ab109624？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab109624 被引用在 14 文献中.

Xu X et al. Increased ATF2 expression predicts poor prognosis and inhibits sorafenib-induced ferroptosis in gastric cancer. Redox Biol 59:102564 (2023). PubMed: 36473315
Cui K et al. Aerobic Exercise Delays Alzheimer's Disease by Regulating Mitochondrial Proteostasis in the Cerebral Cortex and Hippocampus. Life (Basel) 13:N/A (2023). PubMed: 37240849
Kim M et al. Immunoproteasome-specific subunit PSMB9 induction is required to regulate cellular proteostasis upon mitochondrial dysfunction. Nat Commun 14:4092 (2023). PubMed: 37433777
Pan XW et al. Molecular subtyping and characterization of clear cell renal cell carcinoma by tumor differentiation trajectories. iScience 26:108370 (2023). PubMed: 38034348
Nakamura S et al. Ribosomal Stress Couples with the Hypoxia Response in Dec1-Dependent Orthodontic Tooth Movement. Int J Mol Sci 24:N/A (2022). PubMed: 36614058

View all Publications for this product

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1-4 of 4 Abreviews or Q&A

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Skin explants)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citrate

Permeabilization

Specification

Skin explants

Blocking step

BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Aug 03 2023

Application

Western blot

Sample

Human Cell lysate - whole cell (HEK-293)

Gel Running Conditions

Reduced Denaturing (10% Acrylamide)

Loading amount

20 µg

Specification

HEK-293

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 20 2013

Application

Western blot

Sample

Human Cell lysate - whole cell (Human fibroblasts)

Gel Running Conditions

Reduced Denaturing (12.5%)

Loading amount

100000 cells

Specification

Human fibroblasts

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jan 16 2013

Application

Immunoprecipitation

Sample

Human Cell lysate - whole cell (Human fibroblasts)

Total protein in input

100000 cells

Immuno-precipitation step

Protein A/G

Specification

Human fibroblasts

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jan 16 2013

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Key features and details

Related conjugates and formulations

概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Compatible Secondaries

Isotype control

Positive Controls

Recombinant Protein

应用

The Abpromise guarantee

靶标

功能

组织特异性

序列相似性

翻译后修饰

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

文献 (14)

客户评价及客户问答

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Hsp105/HSP110 antibody [EPR4576]

Western blot abreview for Anti-Hsp105/HSP110 antibody [EPR4576]

Western blot abreview for Anti-Hsp105/HSP110 antibody [EPR4576]

Immunoprecipitation abreview for Anti-Hsp105/HSP110 antibody [EPR4576]