Anti-HSF4抗体[2E7] (ab128146)
Key features and details
- Mouse monoclonal [2E7] to HSF4
- Suitable for: WB, ELISA, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-HSF4抗体[2E7] -
描述
小鼠单克隆抗体[2E7] to HSF4 -
宿主
Mouse -
经测试应用
适用于: WB, ELISA, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Purified recombinant fragment of human HSF4 expressed in E. Coli.
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阳性对照
- Hela cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: 99% PBS
contains 0.5% protein stabilizer -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Purified from tissue culture supernatant. -
克隆
单克隆 -
克隆编号
2E7 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab128146于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/2000. Predicted molecular weight: 53 kDa.
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ELISA |
1/10000.
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Flow Cyt |
1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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WB
1/500 - 1/2000. Predicted molecular weight: 53 kDa. |
ELISA
1/10000. |
Flow Cyt
1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
DNA-binding protein that specifically binds heat shock promoter elements (HSE). Isoform HSF4A represses transcription while the isoform HSF4B activates transcription. -
组织特异性
Expressed in heart, skeletal muscle, eye and brain, and at much lower levels in some other tissues. -
疾病相关
Defects in HSF4 are the cause of cataract zonular HSF4-related (CZ-HSF4) [MIM:116800]. A form of zonular cataract. Zonular or lamellar cataracts are opacities, broad or narrow, usually consisting of powdery white dots affecting only certain layers or zones between the cortex and nucleus of an otherwise clear lens. The opacity may be so dense as to render the entire central region of the lens completely opaque, or so translucent that vision is hardly if at all impeded. Zonular cataracts generally do not involve the embryonic nucleus, though sometimes they involve the fetal nucleus. Usually sharply separated from a clear cortex outside them, they may have projections from their outer edges known as riders or spokes.
Defects in HSF4 are the cause of cataract Marner type (CAM) [MIM:116800]. A form of cataract with variable and progressive opacities. Affected individuals present with zonular cataract, although some have nuclear, anterior polar, or stellate cataract. Finger malformation is observed in some kindreds. -
序列相似性
Belongs to the HSF family. -
翻译后修饰
Phosphorylated mainly on serine residues. Phosphorylation on Ser-298 promotes sumoylation on Lys-293.
Isoform HSF4B is constitutively sumoylated. Sumoylation represses the transcriptional activity and is promoted by phosphorylation on Ser-298. HSFA is not sumoylated. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 3299 Human
- Omim: 602438 Human
- SwissProt: Q9ULV5 Human
- Unigene: 512156 Human
- Unigene: 710714 Human
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别名
- Cataract, Marner antibody
- CTM antibody
- Heat shock factor protein 4 antibody
see all
图片
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Anti-HSF4 antibody [2E7] (ab128146) at 1/500 dilution + Human HSF4 recombinant protein.
Developed using the ECL technique.
Predicted band size: 53 kDa -
Flow cytometric analysis of HeLa cells using HSF4 mouse mAb (green) and negative control (red).
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Red: Control Antigen (100ng), Purple: Antigen (10ng), Green: Antigen (50ng), Blue: Antigen (100ng).
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab128146 尚未被引用在任何文献中。