重组Anti-HSF2抗体[EPR1715Y] (ab52758)
![Western blot - Anti-HSF2 antibody [EPR1715Y] (ab52758)](https://www.abcam.cn/ps/products/52/ab52758/Images/ab52758-65188-anti-hsf2-antibody-epr1715y-western-blot.jpg "Western blot - Anti-HSF2 antibody [EPR1715Y] (ab52758)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1715Y] to HSF2
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-HSF2抗体[EPR1715Y]
参阅全部 HSF2 一抗 -
描述
兔单克隆抗体[EPR1715Y] to HSF2 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human HSF2 aa 450-550 (C terminal). The exact sequence is proprietary.
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阳性对照
- HeLa whole cell lysate (ab150035).
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR1715Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52758于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
|
| 说明 |
|---|
|
WB
1/500. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa). |
靶标
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功能
DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. -
序列相似性
Belongs to the HSF family. -
细胞定位
Cytoplasm. Nucleus. Cytoplasmic during normal growth and moves to the nucleus upon activation. - Information by UniProt
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数据库链接
- Entrez Gene: 3298 Human
- Omim: 140581 Human
- SwissProt: Q03933 Human
- Unigene: 158195 Human
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别名
- Heat shock factor protein 2 antibody
- Heat shock transcription factor 2 antibody
- HSF 2 antibody
see all
图片
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Western blot - Anti-HSF2 antibody [EPR1715Y] (ab52758)Anti-HSF2 antibody [EPR1715Y] (ab52758) at 1/500 dilution + Hela cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa -
![Anti-HSF2 antibody [EPR1715Y] (ab52758)](https://www.abcam.cn/ps/products/52/ab52758/Images/ab52758-2-benefits-of-recombinant-antibodies.png)
Anti-HSF2 antibody [EPR1715Y] (ab52758)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab52758 被引用在 2 文献中.
- Yang Y et al. ALG3 Is Activated by Heat Shock Factor 2 and Promotes Breast Cancer Growth. Med Sci Monit 24:3479-3487 (2018). PubMed: 29799832
- Lamoliatte F et al. Large-scale analysis of lysine SUMOylation by SUMO remnant immunoaffinity profiling. Nat Commun 5:5409 (2014). PubMed: 25391492