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重组RabMAb

重组Anti-HSF1 (phospho S326)抗体[EP1713Y] (ab76076)

Reviews (1)Q&A (1)References (53)
Western blot - Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076)
  • Western blot - Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076)
  • Dot Blot - Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076)
  • Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1713Y] to HSF1 (phospho S326)
  • Suitable for: Dot blot, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-HSF1 (phospho S326)抗体[EP1713Y]
    参阅全部 HSF1 一抗

  • 描述

    兔单克隆抗体[EP1713Y] to HSF1 (phospho S326)

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Dot blot, WBmore details
    不适用于: Flow Cyt,ICC/IF,IHC-P or IP

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: HeLa cell lysates and HeLa cell lysate treated with heat (44ºC)

  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab76076于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Dot blot
Use a concentration of 0.338 µg/ml.
WB (1)
1/5000 - 1/10000. Predicted molecular weight: 57 kDa.
说明
Dot blot
Use a concentration of 0.338 µg/ml.
WB
1/5000 - 1/10000. Predicted molecular weight: 57 kDa.
应用说明
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

靶标

  • 功能

    DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.

  • 序列相似性

    Belongs to the HSF family.

  • 结构域

    the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.

  • 翻译后修饰

    Phosphorylated on multiple serine residues, a subset of which are involved in stress-related regulation of transcription activation. Constitutive phosphorylation represses transcriptional activity at normal temperatures. Levels increase on specific residues heat-shock and enhance HSF1 transactivation activity. Phosphorylation on Ser-307 derepresses activation on heat-stress and in combination with Ser-303 phosphorylation appears to be involved in recovery after heat-stress. Phosphorylated on Ser-230 by CAMK2, in vitro. Cadmium also enhances phosphorylation at this site. Phosphorylation on Ser-303 is a prerequisite for HSF1 sumoylation. Phosphorylation on Ser-121 inhibits transactivation and promotes HSP90 binding. Phosphorylation on Thr-142 also mediates transcriptional activity induced by heat. Phosphorylation on Ser-326 plays an important role in heat activation of HSF1 transcriptional activity.
    Sumoylated with SUMO1 and SUMO2 on heat-shock. Heat-inducible sumoylation occurs after 15 min of heat-shock, after which levels decrease and at 4 hours, levels return to control levels. Sumoylation has no effect on HSE binding nor on transcriptional activity. Phosphorylation on Ser-303 is a prerequisite for sumoylation.

  • 细胞定位

    Cytoplasm. Nucleus. Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Heat shock factor 1 antibody
      • Heat shock factor protein 1 antibody
      • Heat shock transcription factor 1 antibody
      • HSF 1 antibody
      • hsf1 antibody
      • HSF1_HUMAN antibody
      • HSTF 1 antibody
      • HSTF1 antibody
      see all

    图片

    • Western blot - Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076)
      Western blot - Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076)
      All lanes : Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076) at 0.169 µg/ml

      Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 2 : HeLa treated with heat at 43? for 30 minutes whole cell lysate
      Lane 3 : HeLa treated with heat at 43? for 30 minutes whole cell lysate. Then the membrane was incubated with alkaline phosphatase.

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.02 µg/ml

      Predicted band size: 57 kDa
      Observed band size: 82 kDa why is the actual band size different from the predicted?



      Blocking/Diluting Buffer and concentration: 5% NFDM /TBST

    • Western blot - Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076)
      Western blot - Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076)
      All lanes : Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076) at 1/10000 dilution (Purified)

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
      Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with heat (42 ?) whole cell lysates
      Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with heat (42 ?) whole cell lysates. Then the membrane was incubated with phosphatase

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 57 kDa
      Observed band size: 82 kDa why is the actual band size different from the predicted?

    • Dot Blot - Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076)
      Dot Blot - Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076)

      Dot blot analysis of HSF1 (phospho S326) peptide (Lane 1), HSF1 non-phospho peptide (Lane 2) labelling HSF1 (phospho S326) with purified ab76076 at 0.338 ug/mL. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at 0.01 ug/mL.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076)
      Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076)

    文献 (53)

    发表研究结果有使用 ab76076？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab76076 被引用在 53 文献中.

    • Du J  et al. Mechanically sensitive HSF1 is a key regulator of left-right symmetry breaking in zebrafish embryos. iScience 26:107864 (2023). PubMed: 37766982
    • Meyers LM  et al. Vulnerability of HIF1α and HIF2α to damage by proteotoxic stressors. Toxicol Appl Pharmacol 445:116041 (2022). PubMed: 35504338
    • Wang C  et al. Triangulating variation in the population to define mechanisms for precision management of genetic disease. Structure 30:1190-1207.e5 (2022). PubMed: 35714602
    • Janus P  et al. HSF1 Can Prevent Inflammation following Heat Shock by Inhibiting the Excessive Activation of the ATF3 and JUN&FOS Genes. Cells 11:N/A (2022). PubMed: 36010586
    • Garofalo M  et al. RNA Molecular Signature Profiling in PBMCs of Sporadic ALS Patients: HSP70 Overexpression Is Associated with Nuclear SOD1. Cells 11:N/A (2022). PubMed: 35053410
    View all Publications for this product

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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-HSF1 (phospho S326) antibody [EP1713Y]

     Good
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (colon)
    Loading amount
    20 µg
    Specification
    colon
    Treatment
    heat shock
    Gel Running Conditions
    Reduced Denaturing (7.5%)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Ms. Young Ju Yoon

    Verified customer

    提交于 Sep 04 2009

    Question

    Inquiry: this antibody, as written on the datasheet, does not react with mouse tissue and murine species.... ONLY human (also on epitomics website) Lindquist (Cell and JCI...) has published the usage of this ab in mice cell line and tissues..... please could you confirm that ab76076 and ab81281 work on WB on mice cells and mice tissues, thank you best regards

    Read More
    Answer

    Thank you for contacting us and bringing this to our attention.


    We have tested both products (ab76076 and ab81281) in mouse and rat samples, via WB.
    The samples we tested were brain, heart, kidney and spleen for each, in mouse and rat. We only saw a few non-specific bands (with both products) in mouse kidney. No bands were observed in the other lysates.
    We do not recommend these products for use in mouse and rat based on this testing.
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

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