AB81281

重组Anti-HSF1 (phospho S303 + S307)抗体[EP1711Y]

Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y]

BOND RX™ Validated
RabMAb
Recombinant
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(5 Publications)

Rabbit Recombinant Monoclonal HSF1 phospho S303 + S307 antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications.

查看别名

HSTF1, HSF1, Heat shock factor protein 1, HSF 1, Heat shock transcription factor 1, HSTF 1

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] (AB81281)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] (AB81281)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling HSF1 with ab81281 at 1/2000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Human breast carcinoma without alkaline phosphatase treatment (A) compared to no signal detected when treated with alkaline phosphatase (B). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval Solution2) for 30 mins. Counterstained with hematoxylin.

Supplier Data

Immunocytochemistry - Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] (AB81281)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling HSF1 with ab81281 at 1/1000 dilution. Confocal image showing cytoplasmic and membranous staining in subsets of HeLa cells. ab195889 AlexaFluor® 594-conjugated Anti-alpha Tubulin was used to counterstain tubulin at 1/200 dilution. The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

Supplier Data

Western blot - Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] (AB81281)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] (ab81281) at 1/50000 dilution

Lane 1:

K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 15 µg

Lane 2:

K-562 whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 76 kDa

false

Exposure time: 100s

Supplier Data

Western blot - Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] (AB81281)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] (ab81281) at 1/50000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Lane 2:

HeLa whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 76 kDa

false

Exposure time: 180s

SDS-PAGE - Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] (AB81281)

SDS-PAGE

Supplier Data

SDS-PAGE - Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] (AB81281)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa cells labelling HSF1 with ab81281 at 1/90 dilution (Red) compared with an isotype control (Black) and an unlabelled control (Cell without incubation with primary antibody and secondary antibody (Blue)). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) was used as the secondary antibody.

不同偶联物与剂型 (1)

Carrier free

Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EP1711Y

亚型

IgG

不含载体蛋白

反应种属

Human

应用

ICC/IF, WB, Flow Cyt (Intra), IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/90", "FlowCytIntra-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Functions as a stress-inducible and DNA-binding transcription factor that plays a central role in the transcriptional activation of the heat shock response (HSR), leading to the expression of a large class of molecular chaperones, heat shock proteins (HSPs), that protect cells from cellular insult damage (PubMed : 11447121, PubMed : 12659875, PubMed : 12917326, PubMed : 15016915, PubMed : 18451878, PubMed : 1871105, PubMed : 1986252, PubMed : 25963659, PubMed : 26754925, PubMed : 7623826, PubMed : 7760831, PubMed : 8940068, PubMed : 8946918, PubMed : 9121459, PubMed : 9341107, PubMed : 9499401, PubMed : 9535852, PubMed : 9727490). In unstressed cells, is present in a HSP90-containing multichaperone complex that maintains it in a non-DNA-binding inactivated monomeric form (PubMed : 11583998, PubMed : 16278218, PubMed : 9727490). Upon exposure to heat and other stress stimuli, undergoes homotrimerization and activates HSP gene transcription through binding to site-specific heat shock elements (HSEs) present in the promoter regions of HSP genes (PubMed : 10359787, PubMed : 11583998, PubMed : 12659875, PubMed : 16278218, PubMed : 1871105, PubMed : 1986252, PubMed : 25963659, PubMed : 26754925, PubMed : 7623826, PubMed : 7935471, PubMed : 8455624, PubMed : 8940068, PubMed : 9499401, PubMed : 9727490). Upon heat shock stress, forms a chromatin-associated complex with TTC5/STRAP and p300/EP300 to stimulate HSR transcription, therefore increasing cell survival (PubMed : 18451878). Activation is reversible, and during the attenuation and recovery phase period of the HSR, returns to its unactivated form (PubMed : 11583998, PubMed : 16278218). Binds to inverted 5'-NGAAN-3' pentamer DNA sequences (PubMed : 1986252, PubMed : 26727489). Binds to chromatin at heat shock gene promoters (PubMed : 25963659). Activates transcription of transcription factor FOXR1 which in turn activates transcription of the heat shock chaperones HSPA1A and HSPA6 and the antioxidant NADPH-dependent reductase DHRS2 (PubMed : 34723967). Also serves several other functions independently of its transcriptional activity. Involved in the repression of Ras-induced transcriptional activation of the c-fos gene in heat-stressed cells (PubMed : 9341107). Positively regulates pre-mRNA 3'-end processing and polyadenylation of HSP70 mRNA upon heat-stressed cells in a symplekin (SYMPK)-dependent manner (PubMed : 14707147). Plays a role in nuclear export of stress-induced HSP70 mRNA (PubMed : 17897941). Plays a role in the regulation of mitotic progression (PubMed : 18794143). Also plays a role as a negative regulator of non-homologous end joining (NHEJ) repair activity in a DNA damage-dependent manner (PubMed : 26359349). Involved in stress-induced cancer cell proliferation in a IER5-dependent manner (PubMed : 26754925).. (Microbial infection) Plays a role in latent human immunodeficiency virus (HIV-1) transcriptional reactivation. Binds to the HIV-1 long terminal repeat promoter (LTR) to reactivate viral transcription by recruiting cellular transcriptional elongation factors, such as CDK9, CCNT1 and EP300.

See full target information HSF1 phospho S303 + S307

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 11:395 PubMed32457290

2020

Decreased expression of FBXW7 by ERK1/2 activation in drug-resistant cancer cells confers transcriptional activation of MDR1 by suppression of ubiquitin degradation of HSF1.

Applications

Unspecified application

Species

Unspecified reactive species

Gil-Im Mun,Eun Choi,Yeongmin Lee,Yun-Sil Lee

American journal of physiology. Heart and circulatory physiology 316:H421-H429 PubMed30499713

2018

Impaired proteostasis in senescent vascular endothelial cells: a perspective on estrogen and oxidative stress in the aging vasculature.

Applications

Unspecified application

Species

Unspecified reactive species

HyunTae V Hwang,Yun Lin,Michelle N Rebuffatti,Darlene T Tran,Lily Lee,Aldrin V Gomes,Chin-Shang Li,Anne A Knowlton

Oncology letters 14:7705-7714 PubMed29250172

2017

Association between integrin-linked kinase and hyperthermia in oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Zhao,Na Liu,Xinhe Hao,Lin Que,Jiyuan Liu,Xiufa Tang

Scientific reports 6:19174 PubMed26754925

2016

IER5 generates a novel hypo-phosphorylated active form of HSF1 and contributes to tumorigenesis.

Applications

Species

Unspecified reactive species

Yoshinori Asano,Tatsuya Kawase,Atsushi Okabe,Shuichi Tsutsumi,Hitoshi Ichikawa,Satoko Tatebe,Issay Kitabayashi,Fumio Tashiro,Hideo Namiki,Tadashi Kondo,Kentaro Semba,Hiroyuki Aburatani,Yoichi Taya,Hitoshi Nakagama,Rieko Ohki

Journal of clinical pathology 66:758-63 PubMed23723304

2013

Correlation between the expression of Id-1 and hyperthermia-associated molecules in oral squamous cell carcinoma.

Applications

IHC

Species

Human

Jun Zhao,Shengzhi Wang,Na Liu,Xiufa Tang

View all publications

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