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Anti-HSD3B2抗体[373CT9.1.3] (ab75710)

Reviews (2) Submit a question References (14)
Western blot - Anti-HSD3B2 antibody [373CT9.1.3] (ab75710)
  • Flow Cytometry (Intracellular) - Anti-HSD3B2 antibody [373CT9.1.3] (ab75710)

Key features and details

  • Mouse monoclonal [373CT9.1.3] to HSD3B2
  • Suitable for: WB, Flow Cyt (Intra)
  • Reacts with: Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-HSD3B2抗体[373CT9.1.3]
    参阅全部 HSD3B2 一抗

  • 描述

    小鼠单克隆抗体[373CT9.1.3] to HSD3B2

  • 宿主

    Mouse

  • 经测试应用

    适用于: WB, Flow Cyt (Intra)more details

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Goat, Horse, Cow, Cat, Dog, Pig, Cynomolgus monkey, Macaque monkey, Rhesus monkey

  • 免疫原

    Synthetic peptide corresponding to Human HSD3B2 aa 1-100 conjugated to keyhole limpet haemocyanin.

  • 阳性对照

    • This antibody gave a positive signal in human adrenal tissue lysate.

  • 常规说明

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab75710于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 42 kDa). Abcam recommends blocking using 3% milk.
Flow Cyt (Intra)
Use 0.1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
说明
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 42 kDa). Abcam recommends blocking using 3% milk.
Flow Cyt (Intra)
Use 0.1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能

    3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.

  • 组织特异性

    Expressed in adrenal gland, testis and ovary.

  • 通路

    Lipid metabolism; steroid biosynthesis.

  • 疾病相关

    Defects in HSD3B2 are the cause of adrenal hyperplasia type 2 (AH2) [MIM:201810]. AH2 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Four clinical types: 'salt wasting' (SW, the most severe type), 'simple virilizing' (SV, less severely affected patients), with normal aldosterone biosynthesis, 'non-classic form' or late onset (NC or LOAH), and 'cryptic' (asymptomatic). In AH2, virilization is much less marked or does not occur. AH2 is frequently lethal in early life.
    Note=Mild HSD3B2 deficiency in hyperandrogenic females is associated with characteristic traits of polycystic ovary syndrome, such as insulin resistance and luteinizing hormon hypersecretion.

  • 序列相似性

    Belongs to the 3-beta-HSD family.

  • 细胞定位

    Endoplasmic reticulum membrane. Mitochondrion membrane.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • 3 beta HSD adrenal and gonadal type antibody
    • 3 beta HSD II antibody
    • 3 beta HSD type II antibody
    • 3 beta hydroxy 5 ene steroid dehydrogenase antibody
    • 3 beta hydroxy Delta(5) steroid dehydrogenase antibody
    • 3 beta hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2 antibody
    • 3 beta-hydroxysteroid dehydrogenase type II, delta 5-delta 4-isomerase type II, 3 beta-HSD type II antibody
    • 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II antibody
    • 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2 antibody
    • 3-beta-HSD II antibody
    • 3-beta-hydroxy-5-ene steroid dehydrogenase antibody
    • 3-beta-hydroxy-Delta(5)-steroid dehydrogenase antibody
    • 3BHS2_HUMAN antibody
    • ADRENAL HYPERPLASIA II antibody
    • beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2 antibody
    • delta 5 delta 4 isomerase type II antibody
    • Delta-5-3-ketosteroid isomerase antibody
    • HSD3B antibody
    • HSD3B2 antibody
    • HSDB antibody
    • HSDB3B antibody
    • hydroxy delta 5 steroid dehydrogenase, 3 beta and steroid delta isomerase 2 antibody
    • Hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2 antibody
    • Progesterone reductase antibody
    • SDR11E2 antibody
    • short chain dehydrogenase/reductase family 11E, member 2 antibody
    • Steroid Delta-isomerase antibody
    see all

图片

  • Western blot - Anti-HSD3B2 antibody [373CT9.1.3] (ab75710)
    Western blot - Anti-HSD3B2 antibody [373CT9.1.3] (ab75710)
    Anti-HSD3B2 antibody [373CT9.1.3] (ab75710) at 1 µg/ml + Human adrenal normal tissue lysate - total protein (ab29249) at 20 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 42 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?
    Additional bands at: 110 kDa, 35 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes


    Blocked using 3% milk.
  • Flow Cytometry (Intracellular) - Anti-HSD3B2 antibody [373CT9.1.3] (ab75710)
    Flow Cytometry (Intracellular) - Anti-HSD3B2 antibody [373CT9.1.3] (ab75710)
    Overlay histogram showing JEG3 cells stained with ab75710 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75710, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

文献 (14)

发表研究结果有使用 ab75710？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab75710 被引用在 14 文献中.

  • Mullen N  et al. Sublethal Hyperthermia Transiently Disrupts Cortisol Steroidogenesis in Adrenocortical Cells. Endocrinology 164:N/A (2023). PubMed: 36932649
  • He W  et al. Panax notoginseng attenuates hypoxia-induced glycolysis in colonic mucosal epithelial cells in DSS-induced colitis. Ann Transl Med 10:218 (2022). PubMed: 35280359
  • Källsten L  et al. Di-n-Butyl Phthalate and Its Monoester Metabolite Impairs Steroid Hormone Biosynthesis in Human Cells: Mechanistic In Vitro Studies. Cells 11:N/A (2022). PubMed: 36230992
  • Wang J  et al. H2 S catalysed by CBS regulates testosterone synthesis through affecting the sulfhydrylation of PDE. J Cell Mol Med 25:3460-3468 (2021). PubMed: 33713531
  • Kurowska P  et al. The role of vaspin in porcine corpus luteum. J Endocrinol 247:283-294 (2020). PubMed: 33108345
View all Publications for this product

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1-2 of 2 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-HSD3B2 antibody [373CT9.1.3]

 Inconclusive
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Testis)
Permeabilization
Yes - 0.25% Triton X-100 for 5 minutes
Specification
Testis
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Brad Hansen

Verified customer

提交于 Oct 07 2022

Western blot abreview for Anti-HSD3B2 antibody [373CT9.1.3]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Dog Tissue lysate - other (adrenal, testes)
Gel Running Conditions
Reduced Denaturing (BioRad Criterion TGX 10%)
Loading amount
20 µg
Specification
adrenal, testes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Sep 02 2020

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