AB75710

Anti-HSD3B2抗体[373CT9.1.3]

Name: HSD3B2抗体[373CT9.1.3] (ab75710)| Abcam中文官网
Brand: Abcam Limited
SKU: AB75710
Price: 4683 CNY
Availability: InStock
Rating: 5 (2 reviews)

Anti-HSD3B2 antibody [373CT9.1.3]

(2 Reviews)

(16 Publications)

Mouse Monoclonal HSD3B2 antibody. Suitable for Flow Cyt (Intra), WB and reacts with Human samples. Cited in 16 publications.

查看别名

HSDB3B, HSD3B2, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II, 3-beta-HSD adrenal and gonadal type, 3-beta-HSD II

2 Images

Flow Cytometry (Intracellular) - Anti-HSD3B2 antibody [373CT9.1.3] (AB75710)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-HSD3B2 antibody [373CT9.1.3] (AB75710)

Overlay histogram showing JEG3 cells stained with ab75710 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75710, 0.1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Project10928****

Western blot - Anti-HSD3B2 antibody [373CT9.1.3] (AB75710)

Blocked using 3% milk.

All lanes:

Western blot - Anti-HSD3B2 antibody [373CT9.1.3] (ab75710) at 1 µg/mL

All lanes:

Human adrenal normal tissue lysate - total protein (<a href='/products/unavailable/human-adrenal-normal-tissue-lysate-total-protein-ab29249'>ab29249</a>) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/5000 dilution

Predicted band size: 42 kDa

Observed band size: 110 kDa,35 kDa,40 kDa

true

Exposure time: 20min

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

373CT9.1.3

亚型

IgG1

轻链类型

kappa

不含载体蛋白

反应种属

Human

应用

WB, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "0.1 µg for 10^6 Cells", "FlowCytIntra-species-notes": "<p><a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p>Abcam recommends blocking using 3% milk.</p>" }, "Cynomolgus monkey": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Macaque monkey": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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产品详情

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.

See full target information HSD3B2

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文献 (16)

Recent publications for all applications. Explore the full list and refine your search

Animals : an open access journal from MDPI 15: PubMed40646785

2025

LncRNA RWDD3 Facilitates Leydig Cell Steroidogenesis by Regulating the miR-1388-5p/NPY1R/cAMP Pathway in Yanshan Cashmere Goats.

Applications

Unspecified application

Species

Unspecified reactive species

Meijing Chen,Xuejiao Yin,Chunhui Duan,Yuchun Xie,Chenghao Ji,Yong Wang,Yueqin Liu,Yingjie Zhang

Biology of reproduction 110:154-168 PubMed37815939

2023

Phoenixin-14 as a novel direct regulator of porcine luteal cell functions†.

Applications

Unspecified application

Species

Unspecified reactive species

Ewa Mlyczyńska,Patrycja Kurowska,Dominika Wachowska,Małgorzata Grzesiak,Joelle Dupont,Agnieszka Rak

Endocrinology 164: PubMed36932649

2023

Sublethal Hyperthermia Transiently Disrupts Cortisol Steroidogenesis in Adrenocortical Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Nathan Mullen,Padraig T Donlon,Jan Sebek,Katen Duffy,Grazia Cappiello,Sarah Feely,Kate M Warde,Brendan Harhen,David P Finn,Paula M O'Shea,Punit Prakash,Martin O'Halloran,Michael C Dennedy

Cells 11: PubMed36230992

2022

Di-n-Butyl Phthalate and Its Monoester Metabolite Impairs Steroid Hormone Biosynthesis in Human Cells: Mechanistic In Vitro Studies.

Applications

Unspecified application

Species

Unspecified reactive species

Liselott Källsten,Paula Pierozan,Jonathan W Martin,Oskar Karlsson

Annals of translational medicine 10:218 PubMed35280359

2022

Panax notoginseng attenuates hypoxia-induced glycolysis in colonic mucosal epithelial cells in DSS-induced colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei He,Huafeng Pan,Ping Tao,Jiang Lin,Beiping Zhang,Shiying Wang

Journal of cellular and molecular medicine 25:3460-3468 PubMed33713531

2021

H S catalysed by CBS regulates testosterone synthesis through affecting the sulfhydrylation of PDE.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Wang,Jing Wang,Tao Shen,Renyun Hong,Shanshan Tang,Xia Zhao

The Journal of endocrinology 247:283-294 PubMed33108345

2020

The role of vaspin in porcine corpus luteum.

Applications

Unspecified application

Species

Unspecified reactive species

Patrycja Kurowska,Ewa Mlyczyńska,Monika Dawid,Małgorzata Grzesiak,Joelle Dupont,Agnieszka Rak

Journal of biochemical and molecular toxicology 34:e22527 PubMed32410385

2020

Quercetin alleviated H O -induced apoptosis and steroidogenic impairment in goat luteinized granulosa cells.

Applications

Unspecified application

Species

Unspecified reactive species

Manman Li,Yang Xue,Hao Yu,Dagan Mao

Biology of reproduction 102:1290-1305 PubMed32149334

2020

Role of vaspin in porcine ovary: effect on signaling pathways and steroid synthesis via GRP78 receptor and protein kinase A†.

Applications

Unspecified application

Species

Unspecified reactive species

Patrycja Kurowska,Ewa Mlyczyńska,Monika Dawid,Joelle Dupont,Agnieszka Rak

Journal of biochemical and molecular toxicology 33:e22329 PubMed30934154

2019

Effects of LPS on the accumulation of lipid droplets, proliferation, and steroidogenesis in goat luteinized granulosa cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhang Maoduo,Yu Hao,Wang Wei,Wang Feng,Mao Dagan

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HSD3B2抗体[373CT9.1.3]

Anti-HSD3B2 antibody [373CT9.1.3]

Flow Cytometry (Intracellular) - Anti-HSD3B2 antibody [373CT9.1.3] (AB75710)

Western blot - Anti-HSD3B2 antibody [373CT9.1.3] (AB75710)

关键信息

宿主种属

克隆

克隆号

亚型

轻链类型

不含载体蛋白

反应种属

应用

免疫原