Anti-HSD17B4抗体[2D3BB5BF10] (ab128565)
Key features and details
- Mouse monoclonal [2D3BB5BF10] to HSD17B4
- Suitable for: WB, ICC/IF, IP
- Knockout validated
- Reacts with: Human
- Isotype: IgG2a
Related conjugates and formulations
概述
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产品名称
Anti-HSD17B4抗体[2D3BB5BF10]
参阅全部 HSD17B4 一抗 -
描述
小鼠单克隆抗体[2D3BB5BF10] to HSD17B4 -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IF, IPmore details -
种属反应性
与反应: Human
不与反应: Mouse, Rat -
免疫原
Tissue, cells or virus. This information is considered to be commercially sensitive.
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阳性对照
- Human liver and heart homogenates; Hela, HepG2, HDFn, HEK293T, Jurkat and SH-SY5Y cell lysate; HDFn cells
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常规说明
This monoclonal antibody to HSD17B4 has been knockout validated in Western blot. The expected band was observed in wild type cells and the band was not seen in knockout cells.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline -
Concentration information loading...
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纯度
Ammonium Sulphate Precipitation -
纯化说明
Purity is near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation. -
克隆
单克隆 -
克隆编号
2D3BB5BF10 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab128565于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 79 kDa.
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ICC/IF |
Use a concentration of 5 µg/ml.
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IP |
Use at an assay dependent concentration.
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说明 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 79 kDa. |
ICC/IF
Use a concentration of 5 µg/ml. |
IP
Use at an assay dependent concentration. |
靶标
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功能
Bifunctional enzyme acting on the peroxisomal beta-oxidation pathway for fatty acids. Catalyzes the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids. -
组织特异性
Present in many tissues with highest concentrations in liver, heart, prostate and testis. -
通路
Lipid metabolism; fatty acid beta-oxidation. -
疾病相关
D-bifunctional protein deficiency
Perrault syndrome 1 -
序列相似性
Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Contains 1 MaoC-like domain.
Contains 1 SCP2 domain. -
细胞定位
Peroxisome. - Information by UniProt
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数据库链接
- Entrez Gene: 3295 Human
- Omim: 601860 Human
- SwissProt: P51659 Human
- Unigene: 406861 Human
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别名
- 12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase antibody
- 17 beta HSD 4 antibody
- 17 beta HSD IV antibody
see all
图片
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Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: HSD17B4 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: A431 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab128565 observed at 85 kDa. Red - loading control, ab181602, observed at 37 kDa.ab128565 was shown to specifically react with HSD17B4 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when HSD17B4 knockout cells were examined. Wild-type and HSD17B4 knockout samples were subjected to SDS-PAGE. Ab128565 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Immunofluorescent staining of HSD17B4 in HDFn cells (human) using ab128565.
The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were then incubated with the antibody (ab128565, 1µg/ml) for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the peroxisome. -
Immunoprecipitation using ab128565.
The protein is found both as a full length peptide and in a cleaved version.
Lane 1: Ladder
Lane 2: Human Liver Homogenate. -
All lanes : Anti-HSD17B4 antibody [2D3BB5BF10] (ab128565) at 1 µg/ml
Lane 1 : ladder
Lane 2 : Human Liver Homoginate at 10 µg
Lane 3 : Human Heart Homogenate at 10 µg
Lane 4 : Hela cell lysate at 10 µg
Lane 5 : HepG2 cell lysate at 10 µg
Lane 6 : HDFn cell lysate at 10 µg
Lane 7 : Hek293T cell lysate at 10 µg
Lane 8 : Jurkat cell lysate at 10 µg
Lane 9 : SH-SY5Y cell lysate at 10 µg
Predicted band size: 79 kDa
实验方案
数据表及文件
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Datasheet download
文献 (5)
ab128565 被引用在 5 文献中.
- Pellegrino E et al. Peroxisomal ROS control cytosolic Mycobacterium tuberculosis replication in human macrophages. J Cell Biol 222:N/A (2023). PubMed: 37737955
- Ranea-Robles P et al. Murine deficiency of peroxisomal L-bifunctional protein (EHHADH) causes medium-chain 3-hydroxydicarboxylic aciduria and perturbs hepatic cholesterol homeostasis. Cell Mol Life Sci 78:5631-5646 (2021). PubMed: 34110423
- Huang H et al. Acetylation-mediated degradation of HSD17B4 regulates the progression of prostate cancer. Aging (Albany NY) 12:14699-14717 (2020). PubMed: 32678070
- Violante S et al. Peroxisomes can oxidize medium- and long-chain fatty acids through a pathway involving ABCD3 and HSD17B4. FASEB J 33:4355-4364 (2019). PubMed: 30540494
- Zhang Y et al. SIRT1 activation alleviates brain microvascular endothelial dysfunction in peroxisomal disorders. Int J Mol Med 44:995-1005 (2019). PubMed: 31257461