Anti-HSD17B4抗体[2D3BB5BF10]

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HSD17B4 antibody [2D3BB5BF10] (AB128565)

Immunofluorescent staining of HSD17B4 in HDFn cells (human) using ab128565.
The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were then incubated with the antibody (ab128565, 1μg/ml) for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the peroxisome.

• IP

Unknown

Immunoprecipitation - Anti-HSD17B4 antibody [2D3BB5BF10] (AB128565)

Immunoprecipitation using ab128565.

The protein is found both as a full length peptide and in a cleaved version.

Lane 1 : Ladder
Lane 2 : Human Liver Homogenate.

All lanes:

Immunoprecipitation - Anti-HSD17B4 antibody [2D3BB5BF10] (ab128565)

Predicted band size: 79 kDa

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• WB

Lab

Western blot - Anti-HSD17B4 antibody [2D3BB5BF10] (AB128565)

Lane 1 : Wild-type HAP1 whole cell lysate (20 μg)
Lane 2 : HSD17B4 knockout HAP1 whole cell lysate (20 μg)
Lane 3 : HeLa whole cell lysate (20 μg)
Lane 4 : A431 whole cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab128565 observed at 85 kDa. Red - loading control, ab181602, observed at 37 kDa.

ab128565 was shown to specifically react with HSD17B4 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when HSD17B4 knockout cells were examined. Wild-type and HSD17B4 knockout samples were subjected to SDS-PAGE. ab128565 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-HSD17B4 antibody [2D3BB5BF10] (ab128565)

Predicted band size: 79 kDa

false

• WB

Unknown

Western blot - Anti-HSD17B4 antibody [2D3BB5BF10] (AB128565)

All lanes:

Western blot - Anti-HSD17B4 antibody [2D3BB5BF10] (ab128565) at 1 µg/mL

Lane 1:

ladder

Lane 2:

Human Liver Homoginate at 10 µg

Lane 3:

Human Heart Homogenate at 10 µg

Lane 4:

Hela cell lysate at 10 µg

Lane 5:

HepG2 cell lysate at 10 µg

Lane 6:

HDFn cell lysate at 10 µg

Lane 7:

Hek293T cell lysate at 10 µg

Lane 8:

Jurkat cell lysate at 10 µg

Lane 9:

SH-SY5Y cell lysate at 10 µg

Predicted band size: 79 kDa

false