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Cardiovascular Blood Serum Proteins

HRP Anti-Transferrin抗体(ab19178)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)

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Key features and details

  • HRP Goat polyclonal to Transferrin
  • Suitable for: ELISA, ICC, WB
  • Reacts with: Human
  • Conjugation: HRP
  • Isotype: IgG

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概述

  • 产品名称

    HRP Anti-Transferrin抗体
    参阅全部 Transferrin 一抗
  • 描述

    HRP山羊多克隆抗体to Transferrin
  • 宿主

    Goat
  • 偶联物

    HRP
  • 经测试应用

    适用于: ELISA, ICC, WBmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Full length protein. This information is proprietary to Abcam and/or its suppliers.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C.
  • 存储溶液

    pH: 6.8
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.005% HEPES, 0.58% Sodium chloride, 0.2% BSA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    Antiserum was solid phase adsorbed to ensure class specificity.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cardiovascular
    • Blood
    • Serum Proteins
    • Cardiovascular
    • Blood
    • Other
    • Signal Transduction
    • Protein Trafficking
    • Organelle Proteins
    • Signal Transduction
    • Metabolism
    • Vitamins / Minerals
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Cardiovascular ELISA kits
    • Isotype/Loading Controls
    • Loading Controls
    • Transferrin

相关产品

  • Recombinant Protein

    • Recombinant human Transferrin protein (ab83560)
  • Related Products

    • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
    • TMB ELISA Substrate (High Sensitivity) (ab171523)
    • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
    • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
    • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
    • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
    • 450 nm Stop Solution for TMB Substrate (ab171529)
    • 650 nm Stop Solution for TMB Substrate (ab171531)
    • Immunoassay Blocking Buffer (ab171534)
    • Immunoassay Blocking (BSA Free) (ab171535)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab19178于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA (1)
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
说明
ELISA
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.

靶标

  • 功能

    Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
  • 组织特异性

    Expressed by the liver and secreted in plasma.
  • 疾病相关

    Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia.
  • 序列相似性

    Belongs to the transferrin family.
    Contains 2 transferrin-like domains.
  • 细胞定位

    Secreted.
  • Target information above from: UniProt accession P02787 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 7018 Human
    • Omim: 190000 Human
    • SwissProt: P02787 Human
    • Unigene: 518267 Human
    • 别名

      • Apotransferrin antibody
      • Beta 1 metal binding globulin antibody
      • Beta-1 metal-binding globulin antibody
      • DKFZp781D0156 antibody
      • PRO1400 antibody
      • PRO1557 antibody
      • PRO2086 antibody
      • Serotransferrin antibody
      • Serotransferrin precursor antibody
      • Siderophilin antibody
      • TF antibody
      • TFQTL1 antibody
      • Transferin antibody
      • Transferrin antibody
      • TRFE_HUMAN antibody
      see all

    实验方案

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (0)

    发表研究结果有使用 ab19178?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab19178 尚未被引用在任何文献中。

    客户评价及客户问答

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    1-2 of 2 Abreviews or Q&A

    ELISA abreview for Anti-Transferrin antibody (HRP)

    Good
    Abreviews
    Abreviews
    Application
    ELISA
    Sample
    Human Purified protein (serum)
    Specification
    serum
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
    Type
    Direct
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Feb 27 2006

    Question

    Do you have a WB protocol: dilution, incubation time, etc. for this antibody? The general guidelines in the website are not enough for customers not used to work with primary antibodies conjugated to HRP.

    Read More

    Abcam community

    Verified customer

    Asked on Jun 11 2006

    Answer

    Thank you for your enquiry. The recommended antibody dilutions for this antibody as detailed on the datasheet are: WB: 1/1000 - 1/10000 (Colorimetric detection), 1/1000 - 1/30000 (Chemiluminescent detection): Predicted molecular weight: 77 kDa. We do not have a recommended protocol specifically for this antibody. However, I would recommend that your customer follows our standard western blot protocol located at the following weblink omitting step (I) and applying the HRP antibody as a secondary antibody following the blocking of the membrane. https://www.abcam.com/assets/pdf/protocols/WB-beginner.pdf I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    回复于 Jun 13 2006

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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