HRP Anti-T7 tag®抗体(ab19291)
Key features and details
- HRP Rabbit polyclonal to T7 tag®
- Suitable for: ELISA, ICC, WB
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG
概述
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产品名称
HRP Anti-T7 tag®抗体
参阅全部 T7 tag® 一抗 -
描述
HRP兔多克隆抗体to T7 tag® -
宿主
Rabbit -
偶联物
HRP -
经测试应用
适用于: ELISA, ICC, WBmore details -
种属反应性
与反应: Species independent -
免疫原
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常规说明
Molar enzyme/antibody protein ratio is 4:1.
T7-TAG® is a trademark of EMD Biosciences, Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.8
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Antibody was isolated by affinity chromatography using the peptide immobilized on solid support and conjugated to horseradish peroxidase (HRP). -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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应用
The Abpromise guarantee
Abpromise™承诺保证使用ab19291于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA | ||
| ICC | ||
| WB |
| 说明 |
|---|
ICC: 1/200 - 1/500.
WB: 1/1000 - 1/30000. Colorimetric detection: 1:1000 - 1:10000, Chemiluminescent detection: 1:1000 - 1:30000
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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相关性
The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins. -
别名
- 10 antibody
- Major capsid protein 10A antibody
- Major capsid protein antibody
see all
图片
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Western blot - HRP Anti-T7 tag® antibody (ab19291)All lanes : HRP Anti-T7 tag® antibody (ab19291) at 1/5000 dilution
Lane 1 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.2 µg
Lane 2 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.1 µg
Lane 3 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.05 µg
Predicted band size: 37 kDa
Exposure time: 3 minutesDetected by chemiluminescence.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab19291 被引用在 1 文献中.
- Pandey N et al. Topoisomerase II SUMOylation activates a metaphase checkpoint via Haspin and Aurora B kinases. J Cell Biol 219:N/A (2020). PubMed: 31712254