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Microbiology Organism Virus RNA Virus single stranded RNA negative strand virus Resp. Syncytial Virus

HRP Anti-Respiratory Syncytial Virus抗体(ab20686)

  • Datasheet
  • SDS
Submit a review Q&A (4)References (3)

Product price, shipping and contact information

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Key features and details

  • HRP Goat polyclonal to Respiratory Syncytial Virus
  • Suitable for: IHC-P, ELISA, Neutralising
  • Reacts with: Respiratory syncytial virus
  • Conjugation: HRP
  • Isotype: IgG

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概述

  • 产品名称

    HRP Anti-Respiratory Syncytial Virus抗体
    参阅全部 Respiratory Syncytial Virus 一抗
  • 描述

    HRP山羊多克隆抗体to Respiratory Syncytial Virus
  • 宿主

    Goat
  • 偶联物

    HRP
  • 经测试应用

    适用于: IHC-P, ELISA, Neutralisingmore details
  • 种属反应性

    与反应: Respiratory syncytial virus
  • 免疫原

    Tissue, cells or virus corresponding to Respiratory Syncytial Virus. Human Respiratory Syncytial Virus isolate.

  • 常规说明

    Covalently coupled to a highly purified preparation of Horseradish Peroxidase (RZ>3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is removed.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C.
  • 存储溶液

    Preservative: 0.002% Thimerosal (merthiolate)
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • 纯度

    IgG fraction
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • single stranded RNA negative strand virus
    • Resp. Syncytial Virus

相关产品

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    • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
    • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
    • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
    • 450 nm Stop Solution for TMB Substrate (ab171529)
    • 650 nm Stop Solution for TMB Substrate (ab171531)
    • Immunoassay Blocking Buffer (ab171534)
    • Immunoassay Blocking (BSA Free) (ab171535)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab20686于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use at an assay dependent dilution.
ELISA
1/200 - 1/1000.
Neutralising
1/256.
说明
IHC-P
Use at an assay dependent dilution.
ELISA
1/200 - 1/1000.
Neutralising
1/256.

靶标

  • 相关性

    Respiratory syncytial virus (RSV) is a major cause of respiratory illness in young children. RSV infection produces a variety of signs and symptoms involving different areas of the respiratory tract, from the nose to the lungs. RSV is a negative sense, enveloped RNA virus. The virion is variable in shape and size with average diameter of between 120 and 300 nm. The 63 kD RSV fusion protein of the RSS 2 strain (subtype A) directs fusion of viral and cellular membranes, results in viral penetration, and can direct fusion of infected cells with adjoining cells, resulting in the formation of syncytia or multi nucleated giant cells.
  • 细胞定位

    Virion. Host cytoplasm
  • 数据库链接

    • Entrez Gene: 2829063 Respiratory syncytial virus
    • 别名

      • RS virus antibody
      • RSV antibody

    实验方案

    • Immunohistochemistry protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (3)

    发表研究结果有使用 ab20686?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab20686 被引用在 3 文献中.

    • Pavot V  et al. A novel vaccine adjuvant based on straight polyacrylate potentiates vaccine-induced humoral and cellular immunity in cynomolgus macaques. Hum Vaccin Immunother 17:2336-2348 (2021). PubMed: 33427044
    • Jones HG  et al. Structural basis for recognition of the central conserved region of RSV G by neutralizing human antibodies. PLoS Pathog 14:e1006935 (2018). PubMed: 29509814
    • Groppo R  et al. Effect of genetic background and delivery route on the preclinical properties of a live attenuated RSV vaccine. PLoS One 13:e0199452 (2018). PubMed: 29920563

    客户评价及客户问答

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    1-4 of 4 Abreviews or Q&A

    Question

    Is the antibody ab20686 specific to RSV subtype A or B?

    Read More

    Abcam community

    Verified customer

    Asked on Feb 27 2018

    Answer

    ab20686 was developed using an "A" strain of RSV. Even though it reacts with both A and B strains, its reactivity is much stronger for A strains and weaker for B strains.

    Read More

    Abcam Scientific Support

    回复于 Feb 27 2018

    Question

    Thank you for replying back. My cells are sitting in 6 well plates, they are cell lines infected with RSV virus and I essentially want to stain the viral plaques for verification.

    After reading the protocol, I understand that this is not the DAB kit which will be solution A and B, am I right.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 26 2012

    Answer

    Thank you for your reply.

    You are correct that the DAB enhancer is not the solution with A & B components. It is to be used in conjunction with a DAB chromogen.

    If there is anything else I can help you with, please let me know.

    Read More

    Abcam Scientific Support

    回复于 Mar 26 2012

    Question

    Hi I have one more question ? what is The DAB Enhancer and what is the protocol for immunostaining if using your antibody for cells that have been fixed in formalin or methanol( non frozen).

    Read More

    Abcam community

    Verified customer

    Asked on Mar 23 2012

    Answer

    Thank you for contacting us.

    The DAB enhancement reagent is for use with DAB chromogens. It can be used as an auxiliary step for richer, chocolate-brown DAB results.


    I have also attached our recommended protocol for ICC staining as you have requested.


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    回复于 Mar 23 2012

    Question

    I have one more question, The antibody is HRP conjugated so you can use for direct immunostaining. You dont need a secondary antibody.Can your technical department confirm this. Also can you confirm if these 2 items are in stock and when will be they delivered. Thanks

    Read More

    Abcam community

    Verified customer

    Asked on Mar 22 2012

    Answer

    Thank you for contacting Abcam.

    AB20686 is an HRP conjugated product and can be used for direct immunostaining. Use of a secondary antibody is un-necessary.

    We have 8 in stock as of Thurday 4pm Pacific time.



    I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

    Read More

    Abcam Scientific Support

    回复于 Mar 22 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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