HRP Anti-polyHistidine抗体[HIS-1] (ab49781)
Key features and details
- HRP Mouse monoclonal [HIS-1] to polyHistidine
- Suitable for: ELISA, Dot blot, WB
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG2a
Related conjugates and formulations
概述
-
产品名称
HRP Anti-polyHistidine抗体[HIS-1] -
描述
HRP小鼠单克隆抗体[HIS-1] to polyHistidine -
宿主
Mouse -
偶联物
HRP -
经测试应用
适用于: ELISA, Dot blot, WBmore details -
种属反应性
与反应: Species independent -
免疫原
Recombinant polyHistidine tagged fusion protein.
-
阳性对照
- Bacteria lysates expressing a recombinant Histidine tagged fusion protein.
-
常规说明
Monoclonal antibody reacting specifically with polyHistidine may be useful in various immunotechniques, to identify the expression of a polyhistidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a polyHistidine tagged fusion protein expressing vectors.
The conjugate was prepared by conjugation of HRP to Mouse monoclonal anti-polyHistidine purified from ascites fluid of the HIS-1 hybridoma. Molar ratio Ab/Enzyme: 0.8 to 1.5 Enzyme activity: at least 400 U/ml
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.0268% PBS, 1% BSA -
Concentration information loading... -
纯度
IgG fraction -
纯化说明
Purified Immunoglobulin -
Primary antibody说明
Monoclonal antibody reacting specifically with polyHistidine may be useful in various immunotechniques, to identify the expression of a polyhistidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a polyHistidine tagged fusion protein expressing vectors. -
克隆
单克隆 -
克隆编号
HIS-1 -
同种型
IgG2a -
研究领域
相关产品
-
Related Products
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab49781于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
|
| Dot blot |
1/2000.
|
|
| WB |
1/2000.
|
| 说明 |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
Dot blot
1/2000. |
|
WB
1/2000. |
靶标
-
相关性
Recombinant DNA technology enables the attachment of genes of interest to specific sequences or genes that can provide 'affinity handles' (tags) designed to enable the selective identification of the protein of interest. These sequences of tails or tags are genetically engineered away from the protein active site, by insertion at the N or C terminus. It has been reported that the addition of a consecutive Histidine amino acid residue tail creates a stable fusion product that does not appear to interfere with the bioactivity of the protein or with the biodistribution of the Histidine tagged product.
数据表及文件
-
SDS download
-
Datasheet download
文献 (2)
ab49781 被引用在 2 文献中.
- Shin J et al. Characterization of a single chain variable fragment of nivolumab that targets PD-1 and blocks PD-L1 binding. Protein Expr Purif 177:105766 (2021). PubMed: 32987122
- Kim YJ et al. Molecular and Structural Characterization of the Tegumental 20.6-kDa Protein in Clonorchis sinensis as a Potential Druggable Target. Int J Mol Sci 18:N/A (2017). PubMed: 28273846