AB197042

重组HRP Anti-Peroxiredoxin 2/PRP抗体[EPR5154]

HRP Anti-Peroxiredoxin 2/PRP antibody [EPR5154]

RabMAb
Recombinant
KO Validated
了解详情

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(1 Publication)

Rabbit Recombinant Monoclonal Peroxiredoxin 2/PRP antibody - conjugated to HRP. Suitable for WB and reacts with Human samples. Cited in 1 publication.

查看别名

NKEFB, TDPX1, PRDX2, Peroxiredoxin-2, Natural killer cell-enhancing factor B, PRP, Thiol-specific antioxidant protein, Thioredoxin peroxidase 1, Thioredoxin-dependent peroxide reductase 1, Thioredoxin-dependent peroxiredoxin 2, NKEF-B, TSA

2 Images

Lab

Western blot - HRP Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (AB197042)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab197042 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab197042) at 1/3000 dilution

Lane 1:

HEK-293 (Human) Whole Cell Lysate (ab52256) at 10 µg

Lane 2:

Western blot - HeLa whole cell lysate (<a href='/products/cell-lysates/hela-whole-cell-lysate-ab150035'>ab150035</a>) at 10 µg

Lane 3:

LNCaP (Human prostate adenocarcinoma cell line) Whole Cell Lysate - clone FGC at 10 µg

Lane 4:

SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg

Predicted band size: 22 kDa

Observed band size: 22 kDa

true

Exposure time: 4min

Lab

Western blot - HRP Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (AB197042)

ab197042 was shown to specifically react with PRDX2 in wild-type HAP1 cells as signal was lost in PRDX2 (Peroxiredoxin 2) knockout cells. Wild-type and PRDX2 (Peroxiredoxin 2) knockout samples were subjected to SDS-PAGE. ab197042 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor^® 680) loading control) were incubated overnight at 4°C at 1/3000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.

All lanes:

Western blot - HRP Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab197042) at 1/3000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

PRDX2 (Peroxiredoxin 2) knockout HAP1 whole cell lysate at 20 µg

Predicted band size: 22 kDa

false

Exposure time: 20min

不同偶联物与剂型 (2)

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Peroxiredoxin 2/PRP antibody [EPR5154]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Peroxiredoxin 2/PRP antibody [EPR5154]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR5154

亚型

IgG

偶联物

HRP

激发波长/发射波长

不含载体蛋白

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

From Jan 2026, QC testing of replenishment batches of this primary conjugate changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, the replenishment lots are subjected to bioanalytical testing only. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle|Store in the dark

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Peroxiredoxin 2 (also known as PRP or PRDX2) is an antioxidant enzyme that plays an important role in reducing peroxides and protecting cells from oxidative damage. The target consisting of approximately 22kDa mass is expressed heavily in erythrocytes but can also be found in other tissues like the heart and liver. PRDX2 belongs to the peroxiredoxin family and its redox activity contributes significantly to cellular homeostasis and defense against oxidative stress.

Biological function summary

Peroxiredoxins like PRDX2 function by breaking down hydrogen peroxide and organic hydroperoxides safeguarding cells from oxidative harm. PRDX2 often forms homodimers or higher-order oligomers which influence its catalytic efficiency and chaperone activity. Within the cell it not only acts independently but also associates with other cellular components reflecting its participation in important cellular processes including cell proliferation and apoptosis.

Pathways

PRDX2 engages significantly within antioxidant defense systems specifically the thioredoxin pathway. It interacts with thioredoxin reductase and thioredoxin to facilitate the reduction of peroxides maintaining the cell’s redox balance. Furthermore PRDX2 intersects with cellular signaling pathways associated with inflammation and the regulation of cell death where proteins like NRF2 and KEAP1 play important roles in managing oxidant-inducible gene expression.

Disruptions in PRDX2 function connect to conditions like cancer and cardiovascular diseases. In cancer altered peroxiredoxin 2 expression correlates to tumor progression and resistance to chemotherapy implicating its interaction with proteins like NF-κB in enhancing cell survival. In cardiovascular diseases oxidative stress mediated by PRDX2 imbalance can lead to myocardial infarction and heart failure linking it to proteins such as SOD2 involved in mitochondrial defense.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2).

See full target information PRDX2

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer research 79:2339-2351 PubMed30862722

2019

Bcl-2 Is a Therapeutic Target for Hypodiploid B-Lineage Acute Lymphoblastic Leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Ernesto Diaz-Flores,Evan Q Comeaux,Kailyn L Kim,Ella Melnik,Kyle Beckman,Kara L Davis,Kevin Wu,Jon Akutagawa,Olga Bridges,Roberta Marino,Margo Wohlfeil,Benjamin S Braun,Charles G Mullighan,Mignon L Loh

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组HRP Anti-Peroxiredoxin 2/PRP抗体[EPR5154]