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Tags & Cell Markers Epitope Tags Myc Tag

HRP Anti-Myc tag抗体(ab1326)

  • Datasheet
  • SDS
Reviews (3)Q&A (3)References (19)

Product price, shipping and contact information

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Key features and details

  • HRP Rabbit polyclonal to Myc tag
  • Suitable for: ELISA, WB, ICC
  • Conjugation: HRP
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
HRP Anti-c-Myc antibody [Y69] (ab205818)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    HRP Anti-Myc tag抗体
    参阅全部 Myc tag 一抗
  • 描述

    HRP兔多克隆抗体to Myc tag
  • 宿主

    Rabbit
  • 偶联物

    HRP
  • 特异性

    In a direct ELISA against 1 ug of C-Myc peptide coupled to BSA, conjugate diluted to 1/20,000 produced a 1.0 OD in a 15 minute reaction with TMB as substrate.
  • 经测试应用

    适用于: ELISA, WB, ICCmore details
  • 免疫原

    Synthetic peptide corresponding to Myc tag conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab13837)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C.
  • 存储溶液

    pH: 7.00
    Preservative: 0.002% Thimerosal (merthiolate)
    Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA
  • Concentration information loading...
  • 纯度

    Affinity purified
  • 纯化说明

    Antibodies were immunoaffinity purified using the peptide conjugated to a solid phase support, and conjugated to horseradish peroxidase.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Tags & Cell Markers
    • Epitope Tags
    • Myc Tag

相关产品

  • Related Products

    • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
    • TMB ELISA Substrate (High Sensitivity) (ab171523)
    • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
    • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
    • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
    • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
    • 450 nm Stop Solution for TMB Substrate (ab171529)
    • 650 nm Stop Solution for TMB Substrate (ab171531)
    • Immunoassay Blocking Buffer (ab171534)
    • Immunoassay Blocking (BSA Free) (ab171535)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab1326于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA
1/5000 - 1/20000.
WB (3)
1/1000 - 1/5000.
ICC
1/200 - 1/500.
说明
ELISA
1/5000 - 1/20000.
WB
1/1000 - 1/5000.
ICC
1/200 - 1/500.

靶标

  • 相关性

    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
  • 细胞定位

    Nuclear
  • 别名

    • c-myc tag antibody
    • Myc Epitope Tag antibody

实验方案

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (19)

发表研究结果有使用 ab1326?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab1326 被引用在 19 文献中.

  • Xing J  et al. Identification of poly(ADP-ribose) polymerase 9 (PARP9) as a noncanonical sensor for RNA virus in dendritic cells. Nat Commun 12:2681 (2021). PubMed: 33976210
  • Fan XJ  et al. NONO phase separation enhances DNA damage repair by accelerating nuclear EGFR-induced DNA-PK activation. Am J Cancer Res 11:2838-2852 (2021). PubMed: 34249431
  • Grigaitis R  et al. Phosphorylation of the RecQ Helicase Sgs1/BLM Controls Its DNA Unwinding Activity during Meiosis and Mitosis. Dev Cell 53:706-723.e5 (2020). PubMed: 32504558
  • Zhang A  et al. EphA2 phosphorylates NLRP3 and inhibits inflammasomes in airway epithelial cells. EMBO Rep 21:e49666 (2020). PubMed: 32352641
  • Lee S  et al. Absence of Cytosolic 2-Cys Prx Subtypes I and II Exacerbates TNF-a-Induced Apoptosis via Different Routes. Cell Rep 26:2194-2211.e6 (2019). PubMed: 30784599
View all Publications for this product

客户评价及客户问答

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1-6 of 6 Abreviews or Q&A

Western blot abreview for Anti-Myc tag antibody (HRP)

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing
Sample
Saccharomyces cerevisiae Cell lysate - whole cell (S. cerevisiae cell lysate)
Specification
S. cerevisiae cell lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 04 2014

Western blot abreview for Anti-Myc tag antibody (HRP)

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Escherichia coli Recombinant protein (BL21(DE3))
Loading amount
0.1 µg
Specification
BL21(DE3)
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jun 09 2010

Western blot abreview for Anti-Myc tag antibody (HRP)

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Escherichia coli Recombinant protein (Expressed in DH10B cell)
Loading amount
0.5 µg
Specification
Expressed in DH10B cell
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 17 2010

Question

I have used this antibody and it is not working well for me in WB. I would like a refund.

Read More

Abcam community

Verified customer

Asked on Jun 14 2012

Answer

Thank you for contacting us.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.


I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

Read More

Abcam Scientific Support

回复于 Jun 14 2012

Question

Hello, is ab1326 the HRP-conjugated version of the ab9106 or of other myc antibodies? I don't quite fully understand the difference between the various primary rabbit polyclonal myc antibodies. Thanks, and happy new year!

Read More

Abcam community

Verified customer

Asked on Jan 05 2012

Answer

Thank you for contacting Abcam regarding our Anti-myc antibodies. I have confirmed that yes, ab1326 is the conjugated version of ab9106. I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

Read More

Abcam Scientific Support

回复于 Jan 05 2012

Question

Sorry to bug you, but I have not received the vial of ab1326 yet. Is it possible for someone to check into it?

Read More

Abcam community

Verified customer

Asked on May 13 2004

Answer

No bother at all! Since I hadn't heard back about my last email, the vial was never shipped. I will have a vial, free of charge, shipped out to you straight away. You will receive it at the latest tomorrow. For your record it is order# 41583. Let me know if you have any more questions or concerns.

Read More

Abcam Scientific Support

回复于 May 13 2004

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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