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HRP Anti-Myc tag抗体[9E10] (ab62928)

Reviews (2)Q&A (1)References (31)
Western blot - HRP Anti-Myc tag antibody [9E10] (ab62928)
  • Western blot - HRP Anti-Myc tag antibody [9E10] (ab62928)

Key features and details

  • HRP Mouse monoclonal [9E10] to Myc tag
  • Suitable for: ELISA, WB
  • Reacts with: Species independent
  • Conjugation: HRP
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 594 Alexa Fluor® 647 APC Biotin Biotin DyLight® 488 DyLight® 550 DyLight® 650 FITC PE SureLight® APC Unconjugated

概述

  • 产品名称

    HRP Anti-Myc tag抗体[9E10]
    参阅全部 Myc tag 一抗

  • 描述

    HRP小鼠单克隆抗体[9E10] to Myc tag

  • 宿主

    Mouse

  • 偶联物

    HRP

  • 经测试应用

    适用于: ELISA, WBmore details

  • 种属反应性

    与反应: Species independent

  • 免疫原

    Synthetic peptide corresponding to Human Myc tag aa 400 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.

  • 阳性对照

    • WB: E.coli Whole Cell Lysate (positive control) expressing 11 different epitope tags (ab5395) and Recombinant E.coli Multi Tag protein (ab36791).

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab62928于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA
1/100 - 1/500.
WB (2)
1/10000 - 1/50000. Detects a band of approximately 41,45 kDa (predicted molecular weight: 49 kDa).
说明
ELISA
1/100 - 1/500.
WB
1/10000 - 1/50000. Detects a band of approximately 41,45 kDa (predicted molecular weight: 49 kDa).

靶标

  • 相关性

    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.

  • 细胞定位

    Nuclear

  • 别名

    • c-myc tag antibody
    • Myc Epitope Tag antibody

图片

  • Western blot - HRP Anti-Myc tag antibody [9E10] (ab62928)
    Western blot - HRP Anti-Myc tag antibody [9E10] (ab62928)
    HRP Anti-Myc tag antibody [9E10] (ab62928) at 1/10000 dilution + Recombinant E. coli Multi Tag protein (ab36791) at 0.5 µg

    Predicted band size: 49 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?


    Exposure time: 4 minutes


    Blocking buffer: 2% BSA
    Gel type: MOPS

  • Western blot - HRP Anti-Myc tag antibody [9E10] (ab62928)
    Western blot - HRP Anti-Myc tag antibody [9E10] (ab62928)
    HRP Anti-Myc tag antibody [9E10] (ab62928) at 1/10000 dilution + E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.1 µg

    Performed under reducing conditions.

    Predicted band size: 49 kDa
    Observed band size: 41 kDa why is the actual band size different from the predicted?

文献 (31)

发表研究结果有使用 ab62928？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab62928 被引用在 31 文献中.

  • Lu Y  et al. A Novel Vpu Adaptive Mutation of HIV-1 Degrades Tetherin in Northern Pig-Tailed Macaques (Macaca leonina) Mainly via the Ubiquitin-Proteasome Pathway and Increases Viral Release. J Virol 97:e0020023 (2023). PubMed: 36971578
  • Daoud M  et al. XIAP promotes melanoma growth by inducing tumour neutrophil infiltration. EMBO Rep 23:e53608 (2022). PubMed: 35437868
  • He W  et al. Targeted demethylation at ZNF154 promotor upregulates ZNF154 expression and inhibits the proliferation and migration of Esophageal Squamous Carcinoma cells. Oncogene 41:4537-4546 (2022). PubMed: 36064578
  • Jin JO  et al. Sequential ubiquitination of p53 by TRIM28, RLIM, and MDM2 in lung tumorigenesis. Cell Death Differ 28:1790-1803 (2021). PubMed: 33328571
  • Shailesh H  et al. Protein arginine methyltransferase 5 (PRMT5) activates WNT/ß-catenin signalling in breast cancer cells via epigenetic silencing of DKK1 and DKK3. J Cell Mol Med 25:1583-1600 (2021). PubMed: 33462997
View all Publications for this product

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-c-Myc antibody [9E10] (HRP)

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Escherichia coli Recombinant protein (BL21(DE3))
Loading amount
0.5 µg
Specification
BL21(DE3)
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 30 2010

Western blot abreview for Anti-c-Myc antibody [9E10] (HRP)

 Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (293T cells)
Loading amount
20 µg
Specification
293T cells
Gel Running Conditions
Reduced Denaturing (Invitrogen NUPAGE Bis-Tris Gel 4-12% Gradient Gel MES Buffer)
Blocking step
Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 25 2010

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