AB185058

重组HRP Anti-muscle Actin抗体[EPR8484] - Loading Control

Name: HRP Anti-muscle Actin抗体[EPR8484] - Loading Control (ab185058)| Abcam中文官网
Brand: Abcam Limited
SKU: AB185058
Price: 6167 CNY
Availability: InStock
Rating: 4 (1 reviews)

HRP Anti-muscle Actin antibody [EPR8484] - Loading Control

RabMAb
Recombinant
了解详情

(1 Review)

(2 Publications)

Rabbit Recombinant Monoclonal alpha skeletal muscle Actin antibody - conjugated to HRP. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

查看别名

ACTA, ACTA1, Alpha-actin-1

1 Images

Lab

Western blot - HRP Anti-muscle Actin antibody [EPR8484] - Loading Control (AB185058)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab185058 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406

All lanes:

Western blot - HRP Anti-muscle Actin antibody [EPR8484] - Loading Control (ab185058) at 1/10000 dilution

Lane 1:

NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg

Lane 2:

Human kidney tissue lysate - total protein (<a href='/products/unavailable/human-kidney-tissue-lysate-total-protein-ab30203'>ab30203</a>) at 20 µg

Lane 3:

Human Fetal Heart at 5 µg

Lane 4:

Human skeletal muscle tissue lysate - total protein (<a href='/products/unavailable/human-skeletal-muscle-tissue-lysate-total-protein-ab29330'>ab29330</a>) at 10 µg

Lane 5:

Skeletal Muscle (Rat) Tissue Lysate at 10 µg

Predicted band size: 42 kDa

Observed band size: 42 kDa

true

Exposure time: 5s

不同偶联物与剂型 (4)

Carrier free

Anti-muscle Actin antibody [EPR8484] - BSA and Azide free
Unconjugated

Anti-muscle Actin antibody [EPR8484] - Loading Control
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-muscle Actin antibody [EPR8484] - Cytoskeleton Marker
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-muscle Actin antibody [EPR8484] - Cytoskeleton Marker

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR8484

亚型

IgG

偶联物

HRP

激发波长/发射波长

不含载体蛋白

反应种属

Mouse, Rat, Human, Human, Mouse, Rat

应用

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle|Store in the dark

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Muscle Actin also known as ACTA1 is a protein that plays a significant role in muscle contraction. It has a molecular weight of approximately 42 kDa. This protein is primarily found in skeletal muscle tissues. Muscle Actin exists in two forms: G-actin a globular monomer and F-actin a filamentous polymer that forms actin filaments. These filaments provide structural support and are involved in muscle contraction through their interaction with myosin.

Biological function summary

Muscle Actin interacts with myosin to facilitate muscle contraction. It is an important component of the thin filament within the sarcomere the basic unit of muscle fiber. Muscle Actin is part of a complex and this complex includes other important proteins like tropomyosin and troponin. These proteins regulate the interaction between actin and myosin enabling the muscle contraction process. Muscle Actin's polymerization and depolymerization dynamics are important for the regulation of cellular movement and structural integrity.

Pathways

Muscle Actin is essential for the actin-myosin contractile machinery in muscle cells which is part of the sarcomere assembly pathway. This process involves other proteins such as myosin heavy chain and tropomyosin which are important for muscle contraction. Another important pathway is the Rho GTPase signaling pathway which controls actin filament assembly and is involved in a variety of cellular processes including muscle contraction and cell motility.

Muscle Actin mutations link to certain muscle-related diseases such as nemaline myopathy and congenital myopathies. Nemaline myopathy involves the presence of rod-like structures in muscle fibers and it results from anomalies in actin filaments. Muscle Actin also associates with cardiac muscle conditions when there are mutations affecting cardiac actin structure or function. This reveals the connection between Muscle Actin tropomyosin and troponin in muscle contraction efficiency and structural integrity contributing to these diseases' pathogenesis.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

See full target information ACTA1

style

left-column

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Artificial organs 44:E406-E418 PubMed32279354

2020

Cold-inducible RNA binding protein agonist enhances the cardioprotective effect of UW solution during extended heart preservation.

Applications

Unspecified application

Species

Unspecified reactive species

Hai-Zhou Pan,Li-Jing Zhang,Yi-Wei Liu,Yong-Nan Li,Zhan-Hao Su,Jian Meng,Hao Zhang

Nature 524:114-8 PubMed26200337

2015

CDA directs metabolism of epigenetic nucleosides revealing a therapeutic window in cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Melania Zauri,Georgina Berridge,Marie-Laëtitia Thézénas,Kathryn M Pugh,Robert Goldin,Benedikt M Kessler,Skirmantas Kriaucionis

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组HRP Anti-muscle Actin抗体[EPR8484] - Loading Control