重组HRP Anti-mH2A1抗体[EPR9359(2)]

• WB

Lab

Western blot - HRP Anti-mH2A1 antibody [EPR9359(2)] (AB209320)

ab209320 was shown to recognize macroH2A.1 in wild-type HAP1 cells as signal was lost at the expected MW in H2AFY (macroH2A.1) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and H2AFY (macroH2A.1) knockout samples were subjected to SDS-PAGE. ab209320 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor^® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.

All lanes:

Western blot - HRP Anti-mH2A1 antibody [EPR9359(2)] (ab209320) at 1/5000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

H2AFY (macroH2A.1) knockout HAP1 whole cell lysate at 20 µg

Predicted band size: 40 kDa

Observed band size: 40 kDa

false

Exposure time: 90s

• WB

Lab

Western blot - HRP Anti-mH2A1 antibody [EPR9359(2)] (AB209320)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab209320 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-mH2A1 antibody [EPR9359(2)] (ab209320) at 1/5000 dilution

Lane 1:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

Western blot - HeLa whole cell lysate (<a href='/products/cell-lysates/hela-whole-cell-lysate-ab150035'>ab150035</a>) at 10 µg

Lane 3:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

Predicted band size: 40 kDa

Observed band size: 40 kDa

true

Exposure time: 12min