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AB197705

重组HRP Anti-Lysozyme抗体[EPR2994(2)]

HRP Anti-Lysozyme antibody [EPR2994(2)]

  • BOND RX™ Validated
  • RabMAb
  • Recombinant
  • KO Validated
  • 了解详情

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(1 Publication)

Rabbit Recombinant Monoclonal Lysozyme antibody - conjugated to HRP. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication.

查看别名

LZM, LYZ, Lysozyme C

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Lysozyme antibody [EPR2994(2)] (AB197705)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Lysozyme antibody [EPR2994(2)] (AB197705)

IHC image of lysozyme staining in a section of formalin-fixed paraffin-embedded normal human tonsil*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab197705, 1/100 dilution, for 15 mins at room temperature.

DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

不同偶联物与剂型 (7)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR2994(2)

亚型

IgG

偶联物

HRP

激发波长/发射波长
不含载体蛋白

No

反应种属

Human

应用

IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

From Jan 2026, QC testing of replenishment batches of this primary conjugate changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, the replenishment lots are subjected to bioanalytical testing only. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle|Store in the dark
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Lysozyme also known as muramidase is a protein with enzymatic activity that breaks down bacterial cell walls by hydrolyzing the glycosidic bonds of peptidoglycan. The molecular weight of lysozyme is approximately 14 kDa. This protein is expressed in various tissues and body fluids including egg whites human saliva tears and milk. Lysozyme acts as an antimicrobial agent and plays a significant role in the innate immune system.
Biological function summary

Lysozyme contributes to host defense by targeting bacteria. It is not part of a complex but functions as a single enzyme. By compromising the integrity of bacterial cell walls it clears pathogens swiftly from the body. Lysozyme activity assays measure its ability to hydrolyze bacterial peptidoglycan reflecting its role in immune defense. Many cells such as neutrophils and macrophages express lysozyme during the immune response aiding in pathogen clearance.

Pathways

Lysozyme is chiefly involved in the antibacterial humoral response pathway. Its action aligns with other antimicrobial proteins such as lactoferrin. Both lysozyme and lactoferrin are integral to the body’s first line of defense against bacterial invasion. In addition lysozyme is relevant in the pathway managing mucosal immunity working in conjunction with proteins that enhance pathogen clearance like defensins.

Lysozyme has links to conditions such as sarcoidosis and hereditary renal amyloidosis. In sarcoidosis altered expression patterns of lysozyme may serve as a marker for disease activity. In hereditary renal amyloidosis mutations in the lysozyme gene result in amyloid fibril formation leading to renal dysfunction. Understanding these connections helps in the diagnosis and treatment of related conditions.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
See full target information LYZ
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Nutrients 16: PubMed39408267

2024

The Influence of Strain and Sex on High Fat Diet-Associated Alterations of Dopamine Neurochemistry in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kristen A Hagarty-Waite,Heather A Emmons,Steve C Fordahl,Keith M Erikson
View all publications
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替代产品

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Primary Antibodies

AB223503

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Primary Antibodies

AB185129

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Primary Antibodies

AB227708

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  • 0
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Primary Antibodies

AB242420

Anti-Lysozyme antibody [SP329] - BSA and Azide free

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Flow Cytometry (Intracellular) - Anti-Lysozyme antibody [SP329] - BSA and Azide free (AB242420)

  • 0
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Primary Antibodies

AB242430

Anti-Lysozyme antibody [SP350] - BSA and Azide free

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|KO Validated

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Primary Antibodies

AB34799

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Primary Antibodies

AB108508

Anti-Lysozyme antibody [EPR2994(2)]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (AB108508)

  • 5
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搭配使用产品

Assay Kits

AB267798

Human Lysozyme ELISA Kit

Recombinant|SimpleStep

ELISA - Human Lysozyme ELISA Kit (AB267798)

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Proteins & Peptides

AB269024

Recombinant Human Tau (phospho ) protein

SDS-PAGE - Recombinant Human Tau (phospho ) protein (AB269024)

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Primary Antibodies

AB130751

Anti-Villin antibody [SP145]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Flow Cytometry (Intracellular) - Anti-Villin antibody [SP145] (AB130751)

  • 5
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Primary Antibodies

AB313128

Alexa Fluor® 555 Anti-Tau (phospho T231) antibody [EPR2488]

RabMAb|Recombinant

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
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Abcam Product Promise

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