HRP抗人IgA抗体[1H9] (ab7383)

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Key features and details

  • HRP Mouse monoclonal [1H9] to Human IgA
  • Suitable for: ELISA, Sandwich ELISA
  • Reacts with: Human
  • Conjugation: HRP
  • Isotype: IgM

概述

  • 产品名称

    HRP抗人IgA抗体[1H9]
    参阅全部 Human IgA 一抗

  • 描述

    HRP小鼠单克隆抗体[1H9] to人IgA

  • 宿主

    Mouse

  • 偶联物

    HRP

  • 特异性

    IgA Fc region.

  • 经测试应用

    适用于: ELISA, Sandwich ELISAmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    IgA from human colostrum.

  • 常规说明

    Concentration varies from lot to lot and can be provided on request.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C.

  • 存储溶液

    pH: 7.40
    Preservative: 0.05% Proclin 300
    Constituents: 0.8% Sodium chloride, 0.02% Potassium chloride, 0.18% Dibasic monohydrogen sodium phosphate, 0.024% Monobasic dihydrogen potassium phosphate
  • Concentration information loading...

  • 纯度

    Protein G purified

  • 纯化说明

    Purified by chromatography on protein G Sepharose. Purity is tested by electrophoresis.

  • 克隆

    单克隆

  • 克隆编号

    1H9

  • 骨髓瘤

    Sp2/0

  • 同种型

    IgM

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7383于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA
Use at an assay dependent concentration.
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [3B7] to Human IgA (ab7400).

Can be used as Detection antibody with recommended pair(s) as capture ab7400.
说明
ELISA
Use at an assay dependent concentration.
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [3B7] to Human IgA (ab7400).

Can be used as Detection antibody with recommended pair(s) as capture ab7400.

靶标

  • 相关性

    Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system.

  • 细胞定位

    Secreted

  • 数据库链接

    • 别名

      • FLJ14473 antibody
      • FLJ35065 antibody
      • FLJ35500 antibody
      • FLJ36402 antibody
      • FLJ39698 antibody
      • FLJ40001 antibody
      • FLJ41548 antibody
      • FLJ41552 antibody
      • FLJ41789 antibody
      • FLJ43248 antibody
      • FLJ43594 antibody
      • FLJ44293 antibody
      • FLJ46028 antibody
      • FLJ46621 antibody
      • FLJ46724 antibody
      • IgA1 antibody
      • Igh2 antibody
      • IGHA1 antibody
      • IGHA2 antibody
      • Immunoglobulin heavy constant alpha 1 antibody
      • Immunoglobulin heavy constant alpha 2 (A2m marker) antibody
      • Immunoglobulin heavy constant alpha 2 antibody
      see all

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (4)

    发表研究结果有使用 ab7383？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab7383 被引用在 4 文献中.

    • Liu L  et al. Genetic regulation of serum IgA levels and susceptibility to common immune, infectious, kidney, and cardio-metabolic traits. Nat Commun 13:6859 (2022). PubMed: 36369178
    • Indenbaum V  et al. Testing IgG antibodies against the RBD of SARS-CoV-2 is sufficient and necessary for COVID-19 diagnosis. PLoS One 15:e0241164 (2020). PubMed: 33227020
    • Memon B  et al. Enhanced differentiation of human pluripotent stem cells into pancreatic progenitors co-expressing PDX1 and NKX6.1. Stem Cell Res Ther 9:15 (2018). PubMed: 29361979
    • Verdier J  et al. Specific IgG response against Mycobacterium avium paratuberculosis in children and adults with Crohn's disease. PLoS One 8:e62780 (2013). ELISA ; Human . PubMed: 23658774

    客户评价及客户问答

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    1-6 of 6 Abreviews or Q&A

    Question

    I want to detect human sIgA in ELISA form. Could I use Anti-Human IgA antibody [1H9] (HRP) (ab7383) for this purpose. Kindly advise.  

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    Answer

    Thank you for contacting us. The Anti-Human IgA antibody [1H9] (HRP), reference ab7383, was raised using human colostrum as immunogen and the colostrum contains mainly secretory IgA. I think this antibody is therefore suitable for your experiments. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Question

    Can you tell me what is the binding constant for these products? 1) ab7383 Human IgA antibody [1H9] (HRP) 2) ab7402 Human IgA antibody [A909] (HRP)

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    Answer

    Thank you for your enquiry. Unfortunately we have not determined the binding constants for these products, nor HRP-conjugation ratio. I am sorry for the inconvenience.

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    Question

    Can you tell me what is the concentration/ratio of HRP conjugation to antibody?

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    Answer

    Thank you for your enquiry. Unfortunately, the concentration/ratio of HRP conjugation to antibody was not determined. However, I can inform you that the antibodies were conjugated to Sigma Horseradish Peroxidase, Type VI (P 8375) by modification of the periodate method. (Wilsom, M.B., Nakane, P.K., “Immunofluorescense and Related Staining Techniques“, Elsevier/North Holland Biomedical Press, Amsterdam, 215 (1978)). I am sorry I can be of more help in this occasion but if there is anything else that I can assist you with, please do not hesitate to contact me.

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    Question

    Customer would like more details about the use of this antibody in ELISA.

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    Answer

    Thank you for your enquiry. We recommend using pair ab7401-ab7383 (capture-detection) for the detection of secretory IgA. You can use any general ELISA protocol and optimize it for the use with these antibodies. If you have any more questions, please contact us again.

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    Question

    Dear Ian, Can you please advice me when the following items will be shipped to us. - Item 1 (TIMP3 Antibody; cat# ab11814 ) from PO AX02134 - Item 16 (Human IgA Antibody [1H9]; cat# ab7383) from AX02125 Thanks for all your help. Kind Regards Ashrina Pratap Inventory & Shipping Manager

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    Answer

    Dear Ashrina We confirm that these items are being shipped today 1st Oct.

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    Question

    Customer would like to use these two antibodies in a sandwich ELISA. Ab7401 is coating, Ab7383 as conjugate; what should be used for detection?

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    Answer

    We recommend using pair ab7401-ab7383 (capture-detection) for the detection of secretory IgA. You can use any general ELISA protocol and optimize it for the use with these antibodies.

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