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AB305721

重组HRP Anti-HNF-4-alpha抗体[EPR3648]

HRP Anti-HNF-4-alpha antibody [EPR3648]

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Rabbit Recombinant Monoclonal HNF-4-alpha antibody - conjugated to HRP.

查看别名

HNF4, NR2A1, TCF14, HNF4A, Hepatocyte nuclear factor 4-alpha, HNF-4-alpha, Nuclear receptor subfamily 2 group A member 1, Transcription factor 14, Transcription factor HNF-4, TCF-14

不同偶联物与剂型 (10)

  • Unconjugated

    Anti-HNF-4-alpha antibody [EPR3648]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-HNF-4-alpha antibody [EPR3648]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-HNF-4-alpha antibody [EPR3648]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-HNF-4-alpha antibody [EPR3648]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-HNF-4-alpha antibody [EPR3648]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-HNF-4-alpha antibody [EPR3648]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-HNF-4-alpha antibody [EPR3648]

  • Carrier free

    Anti-HNF-4-alpha antibody [EPR3648] - BSA and Azide free

  • 578 PE

    PE Anti-HNF-4-alpha antibody [EPR3648]

  • 660 APC

    APC Anti-HNF-4-alpha antibody [EPR3648]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR3648

亚型

IgG

偶联物

HRP

激发波长/发射波长
不含载体蛋白

No

应用

Antibody Labelling, Target Binding Affinity

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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为什么推荐这个?

我们推荐这个产品,因为它经常被用于相同的实验或相关的研究。

我们建议您务必查阅产品说明书,以确认其是否符合您的实验需求;若有疑问,也可联系我们的技术团队获取协助。

产品详情

This conjugated primary antibody is "made to order" and it is released using a quantitative quality control method that ensures binding affinity and labelling efficiency of the conjugate. Via leveraging the power of the Lightning-Link® conjugation technology, Abcam will deliver highly consistent recombinant conjugates in <2 weeks, giving you access to an ever growing portfolio of antibody-label combinations.

For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle|Store in the dark

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

HNF-4-alpha also known as hepatocyte nuclear factor 4-alpha (HNF4A) is a nuclear protein that functions as a transcription factor. It plays an important role in regulating gene expression in the liver kidney and intestine. HNF-4-alpha has a molecular mass of about 54 kDa and is expressed in tissues where it maintains cellular differentiation and metabolic homeostasis. In cellular assays such as ELISA and CHIP detection of HNF-4-alpha provides insights into its expression levels and activity in various biological contexts.
Biological function summary

HNF-4-alpha influences the expression of genes involved in liver and pancreatic function. It acts as a transcriptional regulator that controls fatty acid oxidation gluconeogenesis and insulin secretion. HNF-4-alpha interacts with other proteins to form transcriptional complexes that fine-tune the expression of metabolic enzymes and transport proteins. This protein is key to ensuring that metabolic genes are expressed in a coordinated manner which is essential for maintaining normal metabolic processes.

Pathways

HNF-4-alpha participates in the insulin signaling and lipid metabolism pathways. Through these pathways it interacts with other transcription factors such as HNF-1-alpha and PPAR-alpha which coordinate the regulation of genes involved in glucose metabolism and fatty acid oxidation. HNF-4-alpha plays a critical role by modulating the expression of enzymes like gluconeogenic enzymes in response to metabolic needs which connects it to key metabolic pathways influencing overall energy homeostasis.

HNF-4-alpha is linked to maturity-onset diabetes of the young (MODY1) and certain types of liver diseases. Mutations or dysregulation in HNF-4-alpha can affect the expression of genes essential for insulin production and glucose metabolism leading to disorders like diabetes. Additionally HNF-4-alpha interacts with other proteins and transcription factors implicated in these conditions such as HNF-1-alpha. Understanding the role of HNF-4-alpha in these diseases can provide insights into potential therapeutic targets and intervention strategies.

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

靶点信息

Transcriptional regulator which controls the expression of hepatic genes during the transition of endodermal cells to hepatic progenitor cells, facilitating the recruitment of RNA pol II to the promoters of target genes (PubMed : 30597922). Activates the transcription of CYP2C38 (By similarity). Represses the CLOCK-BMAL1 transcriptional activity and is essential for circadian rhythm maintenance and period regulation in the liver and colon cells (PubMed : 30530698).
See full target information HNF4A

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Alternative Version
Primary Antibodies

AB226487

Anti-HNF-4-alpha antibody [EPR19265-130] - ChIP Grade - BSA and Azide free

primary-antibodies

hnf-4-alpha-antibody-epr19265-130-chip-grade-bsa-and-azide-free-ab226487

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