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HRP Anti-HIV1 p24抗体(ab20365)

Reviews (1)Q&A (1)References (3)

Key features and details

  • HRP Goat polyclonal to HIV1 p24
  • Suitable for: ELISA, ICC, WB
  • Reacts with: Human immunodeficiency virus
  • Conjugation: HRP
  • Isotype: IgG

概述

  • 产品名称

    HRP Anti-HIV1 p24抗体
    参阅全部 HIV1 p24 一抗

  • 描述

    HRP山羊多克隆抗体to HIV1 p24

  • 宿主

    Goat

  • 偶联物

    HRP

  • 特异性

    HIV1 p24 antibody (ab20365) targets p24 (predominate p24 band pattern in Western blot). Does not cross-react with human T or B cells. Antiserum is not reactive with human or bovine sera.

  • 经测试应用

    适用于: ELISA, ICC, WBmore details

  • 种属反应性

    与反应: Human immunodeficiency virus

  • 免疫原

    Full length native protein (purified native p24 from strain IIIb).

  • 常规说明

    Purified IgG fraction covalently coupled to a highly purified preparation of horseradish peroxidase (RZ>3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is removed.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab20365于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA (1)
1/200 - 1/1000.
ICC
1/20 - 1/200.
WB
1/200 - 1/1000. Predicted molecular weight: 56 kDa. Dilution optimised using Chromogenic detection. When using chemiluminescent substrates, the assay will require much less antibody. Depending on the sensitivity of the substrate, the antibody dilution may be 1/10,000 - 1/100,000.
说明
ELISA
1/200 - 1/1000.
ICC
1/20 - 1/200.
WB
1/200 - 1/1000. Predicted molecular weight: 56 kDa. Dilution optimised using Chromogenic detection. When using chemiluminescent substrates, the assay will require much less antibody. Depending on the sensitivity of the substrate, the antibody dilution may be 1/10,000 - 1/100,000.

靶标

  • 相关性

    HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.

  • 细胞定位

    Membrane

  • 数据库链接

    • 别名

      • CA antibody
      • Capsid protein p24 antibody
      • HIV-1 Gag p24 antibody
      • HIV1gp1 antibody
      • Human immunodeficiency virus 1 antibody
      • Human immunodeficiency virus type 1 p24 antibody
      see all

    文献 (3)

    发表研究结果有使用 ab20365？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab20365 被引用在 3 文献中.

    • Tan Z  et al. Eliminating mesothelioma by AAV-vectored, PD1-based vaccination in the tumor microenvironment. Mol Ther Oncolytics 20:373-386 (2021). PubMed: 33614918
    • Peyret H  et al. Improving plant transient expression through the rational design of synthetic 5' and 3' untranslated regions. Plant Methods 15:108 (2019). PubMed: 31548848
    • Guth CA & Sodroski J Contribution of PDZD8 to stabilization of the human immunodeficiency virus type 1 capsid. J Virol 88:4612-23 (2014). ELISA . PubMed: 24554657

    客户评价及客户问答

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    1-2 of 2 Abreviews or Q&A

    ELISA abreview for Anti-HIV1 p24 antibody (HRP)

     Excellent
    Abreviews
    abreview image
    Application
    ELISA
    Sample
    HIV Purified protein (purified from bacteria overexpression)
    Specification
    purified from bacteria overexpression
    Type
    Direct
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Shijian Zhang

    Verified customer

    提交于 Jun 03 2014

    Question

    I'm doing an ELISPOT-type assay with these products and am looking for a protocol.

    Read More
    Answer

    Thank you for your inquiry. I know that you said you're using nitrocellulose, and this protocol uses PVDF, but I hope you can adjust it accordingly. https://www.abcam.com/index.html?pageconfig=resource&rid=11456 I hope this information helps. Please contact us with any other questions.

    Read More

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