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HRP Anti-HIV1 gp120抗体(ab20008)

Submit a review Q&A (3)

Key features and details

  • HRP Goat polyclonal to HIV1 gp120
  • Suitable for: WB, ELISA
  • Reacts with: Human immunodeficiency virus
  • Conjugation: HRP
  • Isotype: IgG

概述

  • 产品名称

    HRP Anti-HIV1 gp120抗体
    参阅全部 HIV1 gp120 一抗

  • 描述

    HRP山羊多克隆抗体to HIV1 gp120

  • 宿主

    Goat

  • 偶联物

    HRP

  • 经测试应用

    适用于: WB, ELISAmore details

  • 种属反应性

    与反应: Human immunodeficiency virus

  • 免疫原

    Tissue, cells or virus corresponding to HIV-1 HIV1 gp120. Purified native gp120 from HIV strain IIIB.

  • 常规说明

    IgG fraction covalently coupled to a highly purified preparation of horseradish peroxidase (RZ>3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. Estimated molar HRP: IgG substitution is 2-3.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab20008于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/20 - 1/200. Predicted molecular weight: 120 kDa.

Dilution optimised using Chromogenic detection.
ELISA
1/200 - 1/1000.
说明
WB
1/20 - 1/200. Predicted molecular weight: 120 kDa.

Dilution optimised using Chromogenic detection.
ELISA
1/200 - 1/1000.

靶标

  • 相关性

    HIV1 is equipped with the envelope gp160 glycoprotein for interaction with Langerhans cells (LCs) and dendritic cells (DCs), the members of the innate immune system, which confront the virus at the portal of virus entry in the human body. These cells are equipped with receptors by which they bind and endocytose the virus. The gp120 glycoprotein is used for binding to CD4 receptor and CCR5 co-receptor of T helper 2 (Th2) cells, and is able to induce FcepsilonRI(+) hematopoietic cells to produce IL4, which inactivates the host adaptive immune response.

  • 细胞定位

    Envelope surface glycoprotein.

  • 数据库链接

    • 别名

      • Glycoprotein 120 antibody
      • gp120 antibody
      • SU antibody
      • Surface protein antibody

    文献 (0)

    发表研究结果有使用 ab20008？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab20008 尚未被引用在任何文献中。

    客户评价及客户问答

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    1-3 of 3 Abreviews or Q&A

    Question

    Product code: 20008

    Inquiry: Hi, We are wondering whether this Ab (Anti-HIV1 gp120 antibody (HRP) (ab20008)) would react with a recombinant truncated gp120 that binds CD4 and CCR5, but lacks V1, V2, V3 and the gp41 binding domain. Thank you,

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    Answer

    Thank you for your inquiry.

    I can confirm that the immunogen for ab20008is purified native gp120 from HIV strain IIIB.

    Since it is a polyclonal antibody we do not know where exactly it bind in the protein.

    We have one mouse monoclonal antibody where the immunogen is is now and a short peptide sequence: ab24745, WQEVGKAMYAPPISGQIRCSSN, corresponding to amino acids 427-448 of Human HIV type I (BH8 isolate) gp120

    https://www.abcam.com/HIV1-gp120-antibody-ED8D4-ab24745.html (or use the following: https://www.abcam.com/HIV1-gp120-antibody-ED8D4-ab24745.html).

    I am sorry that I could not answer your question and hope this information is nevertheless helpful.

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    Question

    Can you tell me if these products are from the same origin (ab21179, ab20105, ab20262, ab20008, ab53840, ab53937).I am looking for the non-conjugated form of ab53937 and ab20105.

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    Answer

    Thank you for your enquiry. These 4 products (ab21179, ab20105, ab20262, ab20008) were produced from the same goat antiserum. Catalog ab21179 is the purified antibody. The purified antibody is then labeled to produce the other catalog numbers: Biotin (ab20105), FITC (ab20262), HRP (ab20008). Meanwhile, ab53840 and ab53937 originate from the same goat antisera. Unfortunately, we do not have the unconjugated version of these antibodies in our catalog but I may check with the originator if it is available. Please let me know if you would like to proceed with an order.

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    Question

    Abs to be used in ELISA, WB against the HIV strain YU2 Gp41: ab20890 ab18633 ab19975 ab24714 Gp120: ab24745 ab20008 ab20105

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    Answer

    Thank you for your enquiry. I have been in touch with the source of these HIV gp120 and gp41 antibodies and I have determine that unfortunately none of them have been tested against the HIV strain YU2. I can tell you that ab18633 is predicted, based on broad reactivity to react with HIV strain YU2. I am sorry that I cannot be of more assistance on this occasion.

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