HRP Anti-HA tag抗体(ab128131)
Key features and details
- HRP Rabbit polyclonal to HA tag
- Suitable for: WB
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG
概述
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产品名称
HRP Anti-HA tag抗体
参阅全部 HA tag 一抗 -
描述
HRP兔多克隆抗体to HA tag -
宿主
Rabbit -
偶联物
HRP -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Species independent -
免疫原
Synthetic peptide corresponding to Human HA tag conjugated to keyhole limpet haemocyanin.
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阳性对照
- This antibody gave a positive signal in the following whole cell lysates: HEK293 (Human embryonic kidney cell line) - expressing HA tag protein; E. coli Positive Control (Escherichia coli ).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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应用
The Abpromise guarantee
Abpromise™承诺保证使用ab128131于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/5000. Detects a band of approximately 48,55 kDa (predicted molecular weight: 48,55 kDa). Abcam recommends using 3% milk as the blocking agent.
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| 说明 |
|---|
|
WB
1/5000. Detects a band of approximately 48,55 kDa (predicted molecular weight: 48,55 kDa). Abcam recommends using 3% milk as the blocking agent. |
靶标
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相关性
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. -
别名
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
see all
图片
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Western blot - HRP Anti-HA tag antibody (ab128131)All lanes : HRP Anti-HA tag antibody (ab128131) at 1/5000 dilution
Lane 1 : HEK-293 (Human) Whole Cell Lysate - expressing HA tag protein (ab20896) at 10 µg
Lane 2 :E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.1 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48,55 kDa
Observed band size: 48,55 kDa why is the actual band size different from the predicted?
Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 150 seconds
Abcam recommends using 3% milk as the blocking agent.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab128131 被引用在 5 文献中.
- Spidlova P et al. Bacteriophage SPO1 protein Gp46 suppresses functions of HU protein in Francisella tularensis. Front Microbiol 14:1330109 (2023). PubMed: 38156016
- Saha B et al. WNK1 is a chloride-stimulated scaffold that regulates mTORC2 activity and ion transport. J Cell Sci 135:N/A (2022). PubMed: 36373794
- Rivas GBS et al. CLOCKWORK ORANGE promotes CLOCK-CYCLE activation via the putative Drosophila ortholog of CLOCK INTERACTING PROTEIN CIRCADIAN. Curr Biol 31:4207-4218.e4 (2021). PubMed: 34331859
- Scholl A et al. Tracheal expression of Osiris gene family in Drosophila. Gene Expr Patterns 28:87-94 (2018). PubMed: 29548969
- Seo AY et al. AMPK and vacuole-associated Atg14p orchestrate µ-lipophagy for energy production and long-term survival under glucose starvation. Elife 6:N/A (2017). WB . PubMed: 28394250