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Tags & Cell Markers Epitope Tags HA Tag

HRP Anti-HA tag抗体(ab1190)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (10)

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ELISA - HRP Anti-HA tag antibody (ab1190)

    Key features and details

    • HRP Chicken polyclonal to HA tag
    • Suitable for: WB, ELISA
    • Reacts with: Species independent
    • Conjugation: HRP
    • Isotype: IgY

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    概述

    • 产品名称

      HRP Anti-HA tag抗体
      参阅全部 HA tag 一抗
    • 描述

      HRP鸡多克隆抗体to HA tag
    • 宿主

      Chicken
    • 偶联物

      HRP
    • 特异性

      In a direct ELISA against 1 µg of HA peptide coupled to BSA as sample, conjugate diluted to 1/10,000 produced a 1.0 OD in a 15 minute reaction with TMB as substrate.

    • 经测试应用

      适用于: WB, ELISAmore details
    • 种属反应性

      与反应: Species independent
    • 免疫原

      Synthetic peptide corresponding to HA tag conjugated to keyhole limpet haemocyanin. Corresponding to the influenza hemagglutinin-HA-epitope.

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C.
    • 存储溶液

      pH: 7.00
      Preservative: 0.002% Thimerosal (merthiolate)
      Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 纯化说明

      Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to horseradish peroxidase.
    • 克隆

      多克隆
    • 同种型

      IgY
    • 研究领域

      • Tags & Cell Markers
      • Epitope Tags
      • HA Tag

    相关产品

    • Related Products

      • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
      • TMB ELISA Substrate (High Sensitivity) (ab171523)
      • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
      • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
      • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
      • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
      • 450 nm Stop Solution for TMB Substrate (ab171529)
      • 650 nm Stop Solution for TMB Substrate (ab171531)
      • Immunoassay Blocking Buffer (ab171534)
      • Immunoassay Blocking (BSA Free) (ab171535)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab1190于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    Use a concentration of 0.05 - 0.5 µg/ml.
    ELISA
    Use a concentration of 1 - 0.2 µg/ml.
    说明
    WB
    Use a concentration of 0.05 - 0.5 µg/ml.
    ELISA
    Use a concentration of 1 - 0.2 µg/ml.

    靶标

    • 相关性

      Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
    • 别名

      • HA epitope tag antibody
      • HA1 antibody
      • HA2 antibody
      • hemagglutinin antibody
      • Hemagglutinin HA1 chain antibody
      • Hemagglutinin HA2 chain antibody
      see all

    图片

    • ELISA - HRP Anti-HA tag antibody (ab1190)
      ELISA - HRP Anti-HA tag antibody (ab1190)

      ELISA - Anti-HA tag antibody (HRP) (ab1190).

    实验方案

    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (10)

    发表研究结果有使用 ab1190?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab1190 被引用在 10 文献中.

    • Macrae TA & Ramalho-Santos M The deubiquitinase Usp9x regulates PRC2-mediated chromatin reprogramming during mouse development. Nat Commun 12:1865 (2021). PubMed: 33767158
    • Li C  et al. Prophylactic In Vivo Hematopoietic Stem Cell Gene Therapy with an Immune Checkpoint Inhibitor Reverses Tumor Growth in Syngeneic Mouse Tumor Models. Cancer Res 80:549-560 (2020). PubMed: 31727629
    • Arora D  et al. Establishment of Proximity-Dependent Biotinylation Approaches in Different Plant Model Systems. Plant Cell 32:3388-3407 (2020). PubMed: 32843435
    • Mori S  et al. The Transcriptional Cofactor VGLL1 Drives Transcription of Human Papillomavirus Early Genes via TEAD1. J Virol 94:N/A (2020). PubMed: 32132238
    • Wang W  et al. TRIM37 deficiency induces autophagy through deregulating the MTORC1-TFEB axis. Autophagy 14:1574-1585 (2018). PubMed: 29940807
    • Li W  et al. Aminopeptidase N is not required for porcine epidemic diarrhea virus cell entry. Virus Res 235:6-13 (2017). PubMed: 28363778
    • Scherer PC  et al. TRPV1 is a physiological regulator of µ-opioid receptors. Proc Natl Acad Sci U S A 114:13561-13566 (2017). PubMed: 29203659
    • van der Kant R  et al. Characterization of the Mammalian CORVET and HOPS Complexes and Their Modular Restructuring for Endosome Specificity. J Biol Chem 290:30280-90 (2015). PubMed: 26463206
    • Klein CJ  et al. Ubiquitin ligase defect by DCAF8 mutation causes HMSN2 with giant axons. Neurology 82:873-8 (2014). PubMed: 24500646
    • Tchekanda E  et al. An infrared reporter to detect spatiotemporal dynamics of protein-protein interactions. Nat Methods 11:641-4 (2014). PubMed: 24747815

    客户评价及客户问答

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    Question

    Hello, I am curious to konw if there is a clone name for this antibody? ab1190 - Chicken polyclonal to HA tag (HRP)

    Read More

    Abcam community

    Verified customer

    Asked on Sep 08 2011

    Answer

    Thank you for contacting us with your question. Since this antibody is a polyclonal, there is not a clone name associated with it. Only monoclonal antibodies will have a clone name. You can keep track of a polyclonal antibody based on their immunogen, host species, purity, or manufacturer and catalog number. I hope this information is helpful, but please let me know if you have any further questions.

    Read More

    Abcam Scientific Support

    回复于 Sep 08 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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