HRP Anti-HA tag抗体[2-2.2.14] (ab173826)
![Western blot - HRP Anti-HA tag antibody [2-2.2.14] (ab173826)](https://www.abcam.cn/ps/products/173/ab173826/Images/ab173826-197546-anti-ha-tag-antibody-2-2214-hrp-western-blot.jpg "Western blot - HRP Anti-HA tag antibody [2-2.2.14] (ab173826)")
Key features and details
- HRP Mouse monoclonal [2-2.2.14] to HA tag
- Suitable for: WB
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG1
概述
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产品名称
HRP Anti-HA tag抗体[2-2.2.14]
参阅全部 HA tag 一抗 -
描述
HRP小鼠单克隆抗体[2-2.2.14] to HA tag -
宿主
Mouse -
偶联物
HRP -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Species independent -
免疫原
Synthetic peptide corresponding to HA tag aa 1-9 conjugated to Ovalbumin.
Sequence:YPYDVPDYA
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阳性对照
- WB: E. coli lysate containing a multi-epitope tagged protein. HEK-293E transfected lysates
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.4 -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
2-2.2.14 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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应用
The Abpromise guarantee
Abpromise™承诺保证使用ab173826于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/500 - 1/2000. Predicted molecular weight: 1 kDa.
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| 说明 |
|---|
|
WB
1/500 - 1/2000. Predicted molecular weight: 1 kDa. |
靶标
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相关性
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. -
别名
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
see all
图片
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Western blot - HRP Anti-HA tag antibody [2-2.2.14] (ab173826)All lanes : HRP Anti-HA tag antibody [2-2.2.14] (ab173826) at 1/1000 dilution
Lane 1 : E. coli lysate containing a multi-epitope tagged protein at 1 µg
Lane 2 : E. coli lysate containing a multi-epitope tagged protein at 0.5 µg
Lane 3 : E. coli lysate containing a multi-epitope tagged protein at 0.25 µg
Lane 4 : E. coli lysate containing a multi-epitope tagged protein at 0.125 µg
Lane 5 : E. coli lysate containing a multi-epitope tagged protein at 0.05 µg
Developed using the ECL technique.
Predicted band size: 1 kDaNote: the predicted MW indicated is for the HA tag.
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![Western blot - HRP Anti-HA tag antibody [2-2.2.14] (ab173826)](https://www.abcam.cn/ps/products/173/ab173826/Images/ab173826-469047-hrp-antiha-tag-antibody-22214-western-blot.jpg)
Western blot - HRP Anti-HA tag antibody [2-2.2.14] (ab173826)All lanes : HRP Anti-HA tag antibody [2-2.2.14] (ab173826) at 0.2 µg/ml
Lane 1 : HEK-293E lysates: untransfected at 40 µg
Lane 2 : HEL-293E lysates: empty vector control at 40 µg
Lane 3 : HEK-293E lysates: HA-H3-FLAG transfected at 40 µg
Lane 4 : HEK-293E lysates: HA-H3-FLAG transfected at 20 µg
Lane 5 : HEK-293E lysates: HA-H3-FLAG transfected at 10 µg
Lane 6 : HEK-293E lysates: HA-H3-FLAG transfected at 5 µg
Lane 7 : HEK-293E lysates: FLAG-p65-HA transfected at 40 µg
Lane 8 : Myc-p65-V5-tagged p65 expressing lysate at 40 µg
Lane 9 : Negative control: Positope at 0.025 µg
Predicted band size: 1 kDa
Observed band size: ~19,~67 kDa why is the actual band size different from the predicted?Antibody specificity demonstrated by detection of different targets fused to HA tag in transiently transfected lysates tested. Relative detection of HA tag was observed across different proteins fused with HA tag in HA-H3-FLAG (Lane 3-6) and FLAG-p65-HA (Lane 7), using ab173826 in Western Blot. Positope lacks HA tag, which has been used as a negative control for HA tag detection. No cross-reactivity was seen with Myc or V5-tagged p65 expressing lysate.
数据表及文件
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Datasheet download
文献 (2)
ab173826 被引用在 2 文献中.
- Li F et al. A novel transcriptional activation mechanism of inulinase gene in Kluyveromyces marxianus involving a glycolysis regulator KmGcr1p with unique and functional Q-rich repeats. Mol Microbiol 117:1063-1079 (2022). PubMed: 35218085
- Xia X et al. A pseudo-receiver domain in Atg32 is required for mitophagy. Autophagy 14:1620-1628 (2018). PubMed: 29909755
