HRP Anti-GAPDH抗体- Loading Control (ab9385)
Key features and details
- HRP Rabbit polyclonal to GAPDH - Loading Control
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
HRP Anti-GAPDH抗体- Loading Control
参阅全部 GAPDH 一抗 -
描述
HRP兔多克隆抗体to GAPDH - Loading Control -
宿主
Rabbit -
偶联物
HRP -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow, Saccharomyces cerevisiae, Xenopus laevis
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免疫原
Full length native protein (purified) corresponding to Human GAPDH.
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阳性对照
- This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; A431; Hek293.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol, 1% BSA -
Concentration information loading... -
纯度
Protein A purified -
Primary antibody说明
This antibody is the HRP conjugated version of ab9485, for more convenient use as a loading control antibody. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Recombinant Protein
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应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (11) |
1/5000. Detects a band of approximately 38 kDa (predicted molecular weight: 36 kDa). Milk blocking may cause 'no bands' problem. Please try BSA as well.
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| 说明 |
|---|
|
WB
1/5000. Detects a band of approximately 38 kDa (predicted molecular weight: 36 kDa). Milk blocking may cause 'no bands' problem. Please try BSA as well. |
靶标
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功能
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. -
通路
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. -
序列相似性
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. -
翻译后修饰
S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
ISGylated. -
细胞定位
Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions. - Information by UniProt
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数据库链接
- Entrez Gene: 281181 Cow
- Entrez Gene: 2597 Human
- Entrez Gene: 100042025 Mouse
- Entrez Gene: 14433 Mouse
- Entrez Gene: 24383 Rat
- Entrez Gene: 685186 Rat
- Entrez Gene: 380259 Xenopus laevis
- Omim: 138400 Human
see all -
别名
- 38 kDa BFA-dependent ADP-ribosylation substrate antibody
- aging associated gene 9 protein antibody
- Aging-associated gene 9 protein antibody
see all
图片
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Western blot - HRP Anti-GAPDH antibody - Loading Control (ab9385)All lanes : HRP Anti-GAPDH antibody - Loading Control (ab9385) at 1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteLane 1 - 4 : GAPDH antibody - Loading Control (ab9385) at 1 ug/ml
Lane 1 : HeLa cell lysate at 20 ug
Lane 2 : A431 cell lysate at 20 ug
Lane 3 : Jurkat cell lysate at 20 ug
Lane 4 : 293 cell lysate at 20 ugPerformed under reducing conditions.
Exposure time : 1 minute
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Western blot - HRP Anti-GAPDH antibody - Loading Control (ab9385)Lanes 1-4 : HRP Anti-GAPDH antibody - Loading Control (ab9385) at 1/5000 dilution ((Blocked in 5% BSA))
Lanes 5-8 : HRP Anti-GAPDH antibody - Loading Control (ab9385) at 1/5000 dilution ((Blocked in 5% MILK))
Lanes 1 & 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252)
Lanes 2 & 6 : Jurkat (Human) Whole Cell Lysate (ab52254)
Lanes 3 & 7 :A-431 whole cell lysate (ab7909)
Lanes 4 & 8 :HEK-293 whole cell lysate (ab7902)
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
The membrane 1-4 was blocked in 5% BSA (1 hour). The membrane 5-8 was blocked in 5% MILK (1 hour). Abcam routinely uses 5% BSA to block, however following recent customer feedback our labs investigated the effect of 5% milk blocking. We can now confirm that milk is not a suitable blocking agent for this antibody and significantly decreases the signal on the membrane.
数据表及文件
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SDS download
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Datasheet download
文献 (101)
ab9385 被引用在 101 文献中.
- Virtanen L et al. Lamin A/C phosphorylation at serine 22 is a conserved heat shock response to regulate nuclear adaptation during stress. J Cell Sci 136:N/A (2023). PubMed: 36695453
- Amaro AA et al. Cerivastatin Synergizes with Trametinib and Enhances Its Efficacy in the Therapy of Uveal Melanoma. Cancers (Basel) 15:N/A (2023). PubMed: 36765842
- Chen X et al. Plasmodium immunotherapy combined with gemcitabine has a synergistic inhibitory effect on tumor growth and metastasis in murine Lewis lung cancer models. Front Oncol 13:1181176 (2023). PubMed: 37916167
- Gehrlein A et al. Targeting neuronal lysosomal dysfunction caused by β-glucocerebrosidase deficiency with an enzyme-based brain shuttle construct. Nat Commun 14:2057 (2023). PubMed: 37045813
- Wu Q et al. Lnc-hipk1 inhibits mouse adipocyte apoptosis as a sponge of miR-497. Biofactors 48:135-147 (2022). PubMed: 34856026