HRP Anti-Fluorescein抗体(ab6656)

Thank you for your inquiry and support of our products. The antibody is only tested and guaranteed against derivatives of FITC (Fluorescein isothiocyanate) but it may react with other forms as well. It has not been tested against 6’-FAM. The antibody is polyclonal and is expected to react with different portions of the molecule, but the specific binding sites are not characterized.

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Without a direct comparison, it is a little difficult to choose between the two. The ab6656 datasheet also has a review of IHC with TSA amplification on frozen tissue, after applying the antibody at a dilution of 1/500.

Neither antibody has received any complaints in the past year but I recommend ab6656 based on the dilutions recommended for the other applications, for instance 1/1000 - 1/5000 for western blotting versus 1/20 - 1/200 for ab19492. This suggests that ab6656 has higher affinity and/or HRP activity, but we cannot be sure without direct comparison using the same samples and supplementary reagents.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for your enquiry. The concentration of this antibody is 1.000 mg/ml, however the working dilution/concentration needs to be determined since the signal depends on several factors (such as expression levels of the target, sample preparation etc.). I would suggest that you should carry out a preliminary experiment with different dilutions of this antibody (i.e.1/100-1/1000) and then adjust the dilution accordingly from the results obtained. I hope this helps and if I can assist further, please do not hesitate to contact me.      

Thank you for your phone call today and for your patience while I was in contact with the lab. The lab confirmed that ab6656 has been tested on FITC-conjugated proteins, and so the antibody is appropriate for your studies. I hope this information is helpful, but please let me know if you have any questions.  

Thank you for your enquiry. Yes, I would consider it highly likely that you will be able to use an anti-fluorescein antibody to immunoprecipitate cross linked chromatin associated with your small molecule. You may also wish to consider NChIP as this has been employed using proteins that directly bind DNA. Furthermore if the context of chromatin is not important you may wish to adopt a DNA IP approach, incubating your small molecule with purified DNA and then performing a modified IP approach. For further details of these approaches please see our protocols web page at: http://ops.abcam.com/index.html?pageconfig=popular_protocols Your assay may require some optimisation given a potential obscuring of the fluorescein epitope under cross linked conditions, but in theory there is no reason why this should not work. Unfortunately we do not have a fluorscein antibody that has been tested using ChIP. In this case I would recommend an antibody that has been characterized and demonstrated to work in as many applications as possible, in particular IP. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Thank you for your enquiry. Yes, the chromogen AEC kit is used after using the HRP conjugated fluorescein antibody in immunohistochemical techniques. If you are doing ELISA then a TMB kit is better.

Sorry, we do not have any data on the specific activity of the HRP.