AB195514

重组HRP Anti-ERG抗体[EPR3864]

HRP Anti-ERG antibody [EPR3864]

RabMAb
Recombinant
了解详情

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(3 Publications)

Rabbit Recombinant Monoclonal ERG antibody - conjugated to HRP. Suitable for WB and reacts with Human samples. Cited in 3 publications.

查看别名

Transcriptional regulator ERG, Transforming protein ERG, ERG

1 Images

Lab

Western blot - HRP Anti-ERG antibody [EPR3864] (AB195514)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab195514 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-ERG antibody [EPR3864] (ab195514) at 1/1000 dilution

All lanes:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 20 µg

Predicted band size: 54 kDa

true

Exposure time: 20min

不同偶联物与剂型 (6)

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-ERG antibody [EPR3864]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-ERG antibody [EPR3864]
578 PE

PE Anti-ERG antibody [EPR3864]
660 APC

APC Anti-ERG antibody [EPR3864]
Unconjugated

Anti-ERG antibody [EPR3864]
Carrier free

Anti-ERG antibody [EPR3864] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR3864

亚型

IgG

偶联物

HRP

激发波长/发射波长

不含载体蛋白

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle|Store in the dark

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ERG or ETS-related gene is a transcription factor that belongs to the ETS (E-26 transformation specific) family. The ERG protein plays an important role in gene regulation involved in cell growth and differentiation. ERG has a molecular mass of approximately 54 kDa. It is expressed in a variety of tissues including the prostate endothelial cells and hematopoietic cells. Researchers often study ERG protein localization through techniques such as ERG IHC to better understand its expression across different tissues for example in the ERG color sections that highlight specific cellular contexts.

Biological function summary

ERG regulates processes critical for normal cellular functions such as proliferation apoptosis and differentiation. It functions as part of a larger transcriptional regulatory complex and interacts with other proteins to exert its effects on target genes. The ERG protein can affect angiogenesis and limb development through its regulation of vascular endothelial cells. By controlling such important cellular processes ERG influences both normal physiological functions and abnormal conditions when dysregulated.

Pathways

ERG is highly significant in the MAPK/ERK signaling pathway which is involved in cell division and survival. ERG interacts with other signaling proteins such as FLI1 and RUNX1 affecting cellular responses and contributing to the regulation of gene expression essential for development and homeostasis. Within the hematopoietic context ERG participates in the regulation of pathways that maintain stem cell populations making it important for normal blood cell development.

ERG is prominently implicated in prostate cancer where gene fusions involving ERG lead to its overexpression and contribute to malignancy. The abnormal expression of ERG in endothelial cells can also have a role in vascular disorders. ERG's interplay with proteins like TMPRSS2 often seen in gene fusions in prostate cancer underlines its role in oncogenesis. Understanding how ERG regulates these pathways and its interactions with other proteins helps in developing targeted therapies for disorders involving ERG dysregulation.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Transcriptional regulator. May participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure.

See full target information ERG

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Nature 629:704-709 PubMed38693257

2024

Structural and molecular basis of choline uptake into the brain by FLVCR2.

Applications

Unspecified application

Species

Unspecified reactive species

Rosemary J Cater,Dibyanti Mukherjee,Eva Gil-Iturbe,Satchal K Erramilli,Ting Chen,Katie Koo,Nicolás Santander,Andrew Reckers,Brian Kloss,Tomasz Gawda,Brendon C Choy,Zhening Zhang,Aditya Katewa,Amara Larpthaveesarp,Eric J Huang,Scott W J Mooney,Oliver B Clarke,Sook Wah Yee,Kathleen M Giacomini,Anthony A Kossiakoff,Matthias Quick,Thomas Arnold,Filippo Mancia

Pathology, research and practice 215:152665 PubMed31585812

2019

Ewing sarcoma with myxoid stroma: Case report of an unusual histological variant.

Applications

Unspecified application

Species

Unspecified reactive species

Borislav A Alexiev,Farres Obeidin,Lawrence J Jennings

Molecular biology of the cell 25:532-47 PubMed24356447

2013

SIRT1 gene expression upon genotoxic damage is regulated by APE1 through nCaRE-promoter elements.

Applications

Unspecified application

Species

Unspecified reactive species

Giulia Antoniali,Lisa Lirussi,Chiara D'Ambrosio,Fabrizio Dal Piaz,Carlo Vascotto,Elena Casarano,Daniela Marasco,Andrea Scaloni,Federico Fogolari,Gianluca Tell

View all publications

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重组HRP Anti-ERG抗体[EPR3864]