AB206272

HRP Anti-ENO1 + ENO2 + ENO3 抗体 [EPR10863(B)]

HRP Anti-ENO1 + ENO2 + ENO3 antibody [EPR10863(B)]

RabMAb
Recombinant
了解详情

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(1 Publication)

Rabbit Recombinant Monoclonal ENO1 antibody - conjugated to HRP. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

查看别名

ENO1L1, MBPB1, MPB1, ENO1, Alpha-enolase, 2-phospho-D-glycerate hydro-lyase, C-myc promoter-binding protein, Enolase 1, MBP-1, MPB-1, Non-neural enolase, Phosphopyruvate hydratase, Plasminogen-binding protein, NNE

1 Images

Lab

Western blot - HRP Anti-ENO1 + ENO2 + ENO3 antibody [EPR10863(B)] (AB206272)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab206272 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-ENO1 + ENO2 + ENO3 antibody [EPR10863(B)] (ab206272) at 1/5000 dilution

Lane 1:

MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Lane 3:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Predicted band size: 47 kDa

Observed band size: 50 kDa

true

Exposure time: 10s

不同偶联物与剂型 (8)

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-ENO1 + ENO2 + ENO3 antibody [EPR10863(B)]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-ENO1 + ENO2 + ENO3 antibody [EPR10863(B)]
660 APC

APC Anti-ENO1 + ENO2 + ENO3 antibody [EPR10863(B)]
578 PE

PE Anti-ENO1 + ENO2 + ENO3 antibody [EPR10863(B)]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-ENO1 + ENO2 + ENO3 antibody [EPR10863(B)]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-ENO1 + ENO2 + ENO3 antibody [EPR10863(B)]
Unconjugated

Anti-ENO1 + ENO2 + ENO3 antibody [EPR10863(B)]
Carrier free

Anti-ENO1 + ENO2 + ENO3 antibody [EPR10863(B)] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR10863(B)

亚型

IgG

偶联物

HRP

激发波长/发射波长

不含载体蛋白

反应种属

Human, Human, Mouse, Rat

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle|Store in the dark

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Enolase 1 or alpha-enolase is an important enzyme in the glycolytic pathway. It catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. We also refer to it as an enolase reaction. ENO1 protein has a molecular mass of approximately 47 kDa and is expressed in most tissues but found most abundantly in the brain muscle and liver. Additionally ENO1 can exist in both cytosolic and plasma membrane-associated forms playing functional roles in different cellular contexts.

Biological function summary

The ENO1 protein serves multiple functions beyond its glycolytic activity. It also acts as a plasminogen receptor on the cell surface aiding in the modulation of pericellular and extracellular matrix degradation processes. Through this role ENO1 influences cell migration and tissue remodeling. This protein can form part of larger protein complexes contributing to its versatility in various cellular functions.

Pathways

ENO1 plays a critical role in glycolysis a central metabolic pathway that converts glucose into pyruvate generating ATP as a result. In this pathway ENO1 closely interacts with other enzymes like phosphoglycerate kinase and pyruvate kinase. Additionally as a plasminogen receptor ENO1 links to the plasminogen activation pathway affecting fibrinolysis and cellular response to tissue injury.

ENO1 is associated with cancer and neurodegenerative diseases. Overexpression or aberrant regulation of ENO1 often correlates with tumor progression and metastasis in cancers such as lung and breast cancer. In neurodegenerative disorders like Alzheimer's disease abnormal functioning of ENO1 can impact brain energy metabolism and neuron degeneration. ENO1's interaction with other proteins such as beta-enolase further connects it to these conditions influencing disease mechanisms and patient outcomes.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Enolase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolysis and the reverse reaction in gluconeogenesis (PubMed : 1369209, PubMed : 29775581). Also involved in various processes such as growth control, hypoxia tolerance and allergic responses (PubMed : 10802057, PubMed : 12666133, PubMed : 2005901, PubMed : 29775581). May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons (PubMed : 12666133). Stimulates immunoglobulin production (PubMed : 1369209).. Isoform MBP-1. Binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.

See full target information ENO1

其他靶点

ENO3,ENO2

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell science 138: PubMed40067248

2025

ATG9A vesicles are a subtype of intracellular nanovesicle.

Applications

Unspecified application

Species

Unspecified reactive species

Mary Fesenko,Daniel J Moore,Peyton Ewbank,Elizabeth Courthold,Stephen J Royle

View all publications

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HRP Anti-ENO1 + ENO2 + ENO3 抗体 [EPR10863(B)]