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HRP Anti-DDDDK tag (Binds to FLAG® tag sequence)抗体[M2] (ab49763)

Reviews (8) Submit a question References (81)
Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)
  • ELISA - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)
  • Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)

Key features and details

  • HRP Mouse monoclonal [M2] to DDDDK tag (Binds to FLAG® tag sequence)
  • Suitable for: WB, ELISA
  • Reacts with: Species independent
  • Conjugation: HRP
  • Isotype: IgG1

选择批间可重复性更高的重组抗体

Product image
HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (ab245895)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术，可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    HRP Anti-DDDDK tag (Binds to FLAG® tag sequence)抗体[M2]
    参阅全部 DDDDK tag (Binds to FLAG® tag sequence) 一抗

  • 描述

    HRP小鼠单克隆抗体[M2] to DDDDK tag (Binds to FLAG® tag sequence)

  • 宿主

    Mouse

  • 偶联物

    HRP

  • 经测试应用

    适用于: WB, ELISAmore details

  • 种属反应性

    与反应: Species independent

  • 免疫原

    Synthetic peptide corresponding to DDDDK tag (Binds to FLAG® tag sequence).
    Sequence:

    DYKDDDDK

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 常规说明

    This product was changed from ascites to tissue culture supernatant on 3rd December 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Lyophilized:Reconstitute to 1.0 ml with distilled deionized water vortex gently and allow vial to sit on ice for 20 minutes. Store at +4°C. Do not freeze.

  • 存放说明

    Shipped at 4°C. Store at +4°C. Store In the Dark.

  • 存储溶液

    pH: 7.40
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.64% Sodium phosphate, 0.58% Sodium chloride, 1.5% BSA, 1.71% Sucrose
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    M2

  • 同种型

    IgG1

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab49763于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (7)
1/500 - 1/10000.
ELISA (1)
1/20000.
说明
WB
1/500 - 1/10000.
ELISA
1/20000.

靶标

  • 相关性

    This is a useful tool for the localisation and characterisation of DDDDK tagged proteins (Binds to FLAG® tag sequence).

  • 别名

    • DDDDK epitope tag antibody
    • DDDK antibody
    • ddk antibody
    • DYKDDDDK (also known as the FLAG® tag sequence) antibody
    • DYKDDDDK antibody
    • DYKDDDDK epitope tag antibody
    • DYKDDDDK tag antibody
    • ECS epitope tag antibody
    • ECS tag antibody
    • Enterokinase Cleavage Site epitope tag antibody
    • Enterokinase Cleavage Site tag antibody
    • FLAG® antibody
    • FLAG® tag antibody
    see all

图片

  • Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)
    Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)This image is courtesy of an anonymous Abreview
    All lanes : HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763) at 1/3000 dilution

    All lanes : Saccharomyces cerevisiae Cell lysate - whole cell (S. cerevisiae cell lysate).

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 45 seconds


    Sample 1, 2, 3, 4, 5, 6 are the lysates from the strains expressing the tagged protein.

    5% milk blocking was used for 1 hour.

    See Abreview

  • ELISA - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)
    ELISA - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)This image is courtesy of an anonymous Abreview

    ELISA for the detection of FLAG®-tag purified protein from Escherichia coli, using ab28947 (1/5000). Please refer to abreview for further experimental details.

    Please note that in order to show a clearer image, we have brightened the original image.

    See Abreview

  • Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)
    Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)This image is courtesy of an anonymous Abreview

    Saccharomyces cerevisiae purified FLAG®-tag protein was detected by using ab49763 (1/1000). Please refer to abreview for further experimental details.

    See Abreview

文献 (81)

发表研究结果有使用 ab49763？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab49763 被引用在 81 文献中.

  • Zhu W  et al. In-frame deletion of SMC5 related with the phenotype of primordial dwarfism, chromosomal instability and insulin resistance. Clin Transl Med 13:e1007 (2023). PubMed: 36627765
  • Meyer M  et al. Structure-function relationships of the disease-linked A218T oxytocin receptor variant. Mol Psychiatry 27:907-917 (2022). PubMed: 34980886
  • Tuzlakoğlu Öztürk M & Güllülü Ö Dimerization underlies the aggregation propensity of intrinsically disordered coiled-coil domain-containing 124. Proteins 90:218-228 (2022). PubMed: 34369007
  • Umansky C  et al. Endogenous formaldehyde scavenges cellular glutathione resulting in redox disruption and cytotoxicity. Nat Commun 13:745 (2022). PubMed: 35136057
  • Ma R  et al. LGL1 binds to Integrin β1 and inhibits downstream signaling to promote epithelial branching in the mammary gland. Cell Rep 38:110375 (2022). PubMed: 35172155
View all Publications for this product

客户评价及客户问答

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1-8 of 8 Abreviews or Q&A

Western blot abreview for Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (HRP)

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Saccharomyces cerevisiae Cell lysate - whole cell (S. cerevisiae cell lysate)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
10 µg
Specification
S. cerevisiae cell lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 04 2014

Western blot abreview for Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (HRP)

 Good
Abreviews
abreview image
Application
Western blot
Sample
Human Purified protein (NIH3T3 cells)
Gel Running Conditions
Reduced Denaturing (3-8 % Tris-Acetate Gel)
Loading amount
5e+006 cells
Treatment
10 mM Sodium Butyrate for 24 hrs, 500 ug/ml Geneticin for 48 hrs
Specification
NIH3T3 cells
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jan 07 2011

Western blot abreview for Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (HRP)

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Escherichia coli Cell lysate - whole cell (chimeric protein)
Gel Running Conditions
Reduced Denaturing (4-12% bis tris)
Loading amount
2 µg
Specification
chimeric protein
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 17 2010

Western blot abreview for Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (HRP)

 Good
Abreviews
abreview image
Application
Western blot
Sample
Chinese hamster Cell lysate - whole cell (CHO-S, transfected with plasmid containing insert)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
125000 cells
Specification
CHO-S, transfected with plasmid containing insert
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jun 15 2010

Western blot abreview for Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (HRP)

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Escherichia coli Purified protein (BL21(DE3))
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
1 µg
Specification
BL21(DE3)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Apr 27 2010

ELISA abreview for Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (HRP)

 Excellent
Abreviews
abreview image
Application
ELISA
Sample
Escherichia coli Purified protein (Mach1 (Invitrogen))
Specification
Mach1 (Invitrogen)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Type
Direct
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 19 2009

Western blot abreview for Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (HRP)

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Saccharomyces cerevisiae Purified protein (BJ5453)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
1 µg
Specification
BJ5453
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jan 14 2009

Western blot abreview for Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (HRP)

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Escherichia coli Purified protein (Mach1)
Gel Running Conditions
Reduced Denaturing (12% SDS PAGE)
Loading amount
0.01 µg
Specification
Mach1
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Dec 16 2008

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